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Towards Genetically Designed Tissues for Regenerative Medicine

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Fundamentals of Tissue Engineering and Regenerative Medicine

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Abstract

Integration of fundamental research, elucidating cell differentiation of synthetically designed trigger-inducible promoters and of viral transduction technology, is the basis of tissue engineering for regenerative medicine [29]. Fundamental research has revealed numerous factors that play a role in targeted cell differentiation, such as bone morphogenetic proteins, BMPs [37], angiogenesis-related factors (VEGF, angiopoietin [71]) or cell-cycle regulators (cyclindependent kinase inhibitors [10]). These proteins can be introduced efficiently and safely into various cell types through the development of viral vectors, which are most commonly derived from adeno- and retroviruses [74]. However, as in small-moleculebased medicine, the dose of the therapeutic agent (i.e., the transgene) differentiates a poison from a remedy (Paracelsus, 1493–1541), thereby necessitating genetic tools for adjustment of the transgene expression levels into the therapeutic window. To achieve this, a variety of adjustable expression systems have been designed and have proven essential in almost all fields of mammalian cell technology including functional genomics [23], gene therapy [74], tissue engineering [73], drug discovery [2, 88], synthetic biology [32, 36] as well as the manufacturing of protein therapeutics [6, 13]. This chapter provides an overview of current inducible expression systems with a special focus on systems suitable for tissue engineering applications. Together with an overview of currently used viral vectors this chapter explains how the discovered proteins can be applied in gene-based tissue engineering for regenerative medicine.

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Authors and Affiliations

  1. Institute for Chemical and Bioengineering, HCI F115, ETH Zurich, Wolfgang-Pauli-Straße 10, 8093, Zurich, Switzerland

    Wilfried Weber & Martin Fussenegger

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  1. Wilfried Weber
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  2. Martin Fussenegger
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  1. Clinic for Maxillofacial and Plastic Facial Surgery, Heinrich Heine University Düsseldorf, Moorenstraße 5, 40225, Düsseldorf, Germany

    Ulrich Meyer  & Jörg Handschel  & 

  2. Department of Experimental Maxillofacial Surgery, Biomineralisation and Tissue Engineering Group, University of Münster, Waldeyerstraße 30, 48149, Münster, Germany

    Hans Peter Wiesmann

  3. Department of Internal Medicine – Cardiology, University Hospital Marburg, Baldingerstraße 1, 35033, Marburg, Germany

    Thomas Meyer

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Weber, W., Fussenegger, M. (2009). Towards Genetically Designed Tissues for Regenerative Medicine. In: Meyer, U., Handschel, J., Wiesmann, H., Meyer, T. (eds) Fundamentals of Tissue Engineering and Regenerative Medicine. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-77755-7_9

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