Skip to main content
SpringerLink
Log in

Magnetic Resonance of Mouse Models of Cardiac Disease

  • Chapter
Molecular Imaging II

Part of the book series: Handbook of Experimental Pharmacology ((HEP,volume 185/2))

Abstract

In recent years magnetic resonance imaging (MRI) has become the noninvasive standard for the quantitative evaluation of cardiac function, masses, and infarct size. Wall motion analysis is used to display myocardial dysfunction and microcirculatory deficits can be displayed by perfusion imaging and quantification of the myocardial regional blood volume. Magnetic resonance spectroscopy (MRS) also provides quantitative information on cardiac energetics and, in combination with MRI, insights into cardiac structure and function. The use of both techniques permits complementary data collection within the same experimental setup.

Nevertheless, it should be mentioned that MR does not directly visualize genes or gene product expression but morphological or bioenergetical outcomes of gene expression instead.

In conclusion, cardiac MR is a valuable tool applicable to mouse phenotyping and, also, can be applied to assess the effects of therapeutic agents. Thus, MR of mouse models of cardiac disease has great potential to substantially contribute to the understanding of the underlying pathomechanisms and can help to evaluate new therapy options.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 259.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 329.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 329.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Axel L, Dougherty L (1989) Heart wall motion: improved method of spatial modulation of magnetization for MR imaging. Radiology 172:349–350

    PubMed  CAS  Google Scholar 

  • Bashir A, Bao J, Simons M, Post M, Burstein D (2000) MR imaging of perfusion defects in mice. Proc Intl Soc Magn Reson Med 8:726

    Google Scholar 

  • Brau AC, Wheeler CT, Hedlund LW, Johnson GA (2002) Fiber-optic stethoscope: a cardiac monitoring and gating system for magnetic resonance microscopy. Magn Reson Med 47:314–321

    Article  PubMed  Google Scholar 

  • Cassidy PJ, Schneider JE, Grieve SM, Lygate C, Neubauer S, Clarke K (2004) Assessment of motion gating strategies for mouse magnetic resonance at high magnetic fields. J Magn Reson Imaging 19:229–237

    Article  PubMed  Google Scholar 

  • Chacko VP, Aresta F, Chacko SM, Weiss RG (2000) MRI/MRS assessment of in vivo murine cardiac metabolism, morphology, and function at physiological heart rates. Am J Physiol Heart Circ Physiol 279:H2218–H2224

    PubMed  CAS  Google Scholar 

  • Choudhury RP, Aguinaldo JG, Rong JX, Kulak JL, Kulak AR, Reis ED, Fallon JT, Fuster V, Fisher EA, Fayad ZA (2002) Atherosclerotic lesions in genetically modified mice quantified in vivo by non-invasive high-resolution magnetic resonance microscopy. Atherosclerosis 162:315–321

    Article  PubMed  CAS  Google Scholar 

  • Detre J, Leigh JS, Williams DS, Koretsky AP. Perfusion Imaging (1992) Magn Reson Med 23: 37–45

    Article  PubMed  CAS  Google Scholar 

  • Engelhardt S, Hein L, Wiesmann F, Lohse MJ (1999) Progressive hypertrophy and heart failure in beta1-adrenergic receptor transgenic mice. Proc Natl Acad Sci U S A 96:7059–7064

    Article  PubMed  CAS  Google Scholar 

  • Fayad ZA, Fallon JT, Shinnar M, Whrli S, Dansky HM, Poon M, Badimon JJ, Charlton SA, Fisher EA, Breslow JL, Fuster V (1998) Noninvasive in vivo high-resolution magnetic resonance imaging of atherosclerotic lesions in genetically engineered mice. Circulation 98: 1541–1547

    PubMed  CAS  Google Scholar 

  • Fishbein KW, McConville P, Spencer RG (2001) The lever-coil: a simple, inexpensive sensor for respiratory and cardiac motion in MRI experiments. Magn Reson Imaging 19:881–889

    Article  PubMed  CAS  Google Scholar 

  • Franco F, Dubois SK, Peshock RM, Shohet RV (1998) Megnetic resonance imaging accurately estimates LV mass in a transgenic mouse model of cardiac hypertrophy. Am J Physiol 274: H679–H683

    PubMed  CAS  Google Scholar 

  • Franco F, Thomas GD, GiroirB, Bryant D, Bullock MC, Chwialkowski MC, Victor RG, Peshock RM (1999) Magnetic resonance imaging and invasive evaluation of development of heart failure in transgenic mice with myocardial expression of tumor necrosis factor-α. Circulation 99: 448–454

    PubMed  CAS  Google Scholar 

  • Haase A, Frahm J, Matthaei M, Hänicke W, Merboldt KD (1986) FLASH imaging: rapid NMR imaging using low flip angle pulses. J Magn Reson 67:258–266

    CAS  Google Scholar 

  • Hennig J, Nauerth A, Friedburg H, Ratzel D (1984) New rapid imaging procedure for nuclear spin tomography. Radiologe 24:579–580

    PubMed  CAS  Google Scholar 

  • Henson RE, Song SK, Pastorek JS, Lorenz CH (2000) Left ventricular torsion is equal in mice and humans. Am J Physiol 278:H1117–H1123

    CAS  Google Scholar 

  • Jerosch-Herold M, Wilke N (1998) Magnetic resonance qunatification of the myocardial perfusion reserve with a fermi function model for constrained deconvolution. Med Phys 25:73–84

    Article  PubMed  CAS  Google Scholar 

  • Kahler E, Waller C, Rommel E, Belle V, Hiller KH, Voll S, Bauer WR, Haase A (1999) Perfusion-corrected mapping of cardiac regional blood volume in rats in vivo. Magn Reson Med 42: 500–506

    Article  PubMed  CAS  Google Scholar 

  • Kubota T, McTierman CF, Frye CS, Slawson SE, Lemster HB, Koretsky AP, Demetris AJ, Feldman AM (1997) Dilated cardiomyopathy in transgenic mice with cardiac-specific overexpression of tumor necrosis factor-alpha. Circ Res 81:627–635

    PubMed  CAS  Google Scholar 

  • Lemieux SK, Glover GH (1996) An infrared device for monitoring the respiration of small rodents during magnetic resonance imaging. J Magn Reson Imaging 6:561–564

    Article  PubMed  CAS  Google Scholar 

  • Maestri R, Milia AF, Salis MB, Graiani G, Lagrasta C, Monica M, Corradi D, Emanueli C, Madeddu P (2003) Cardiac hypertrophy and microvascular deficit in kinin B2 receptor knockout mice. Hypertension 41:1151–1155

    Article  PubMed  CAS  Google Scholar 

  • Manning WJ, Wei JY, Fossel ET, Burstein D (1990) Measurement of left ventricular mass in rats using electrocardiogram-gated magnetic resonance imaging. Am J Physiol 258:H1181–H1186

    PubMed  CAS  Google Scholar 

  • McKibben CK, Reo NV (1992) A piezoelectric respiratory monitor for in vivo NMR. Magn Reson Med 27:338–342

    Article  PubMed  CAS  Google Scholar 

  • Merx MW, Flogel U, Stumpe T, Godecke A, Decking UK, Schrader J (2001) Myoglobin facilitates oxygen diffusion. FASEB J 15:1077–1079

    PubMed  CAS  Google Scholar 

  • Minard KR, Wind RA, Phelps RL (1998) A compact respiratory-triggering device for routine microimaging of laboratory mice. J Magn Reson Imaging 8:1343–1348

    Article  PubMed  CAS  Google Scholar 

  • Nahrendorf M, Hu K, Fraccarollo D, Hiller KH, Haase A, Bauer WR, Ertl G (2003) Time course of right ventricular remodeling in rats with experimental myocardial infarction. Am J Physiol Heart Circ Physiol 284:H241–H248

    PubMed  CAS  Google Scholar 

  • Nahrendorf M, Hiller KH, Hu K, Ertl G, Haase A, Bauer WR (2003) Cardiac magnetic resonance imaging in small animal models of human heart failure. Med Image Anal 7:369–375

    Article  PubMed  CAS  Google Scholar 

  • Nahrendorf M, Streif JUG, Hiller KH, Hu K, Nordbeck P, Ritter O, Sosnovik D, Bauer L, Neubauer S, Jakob PM, Ertl G, Spindler M, Bauer WR (2006) Multimodal functional cardiac MRI in creatine kinase-deficient mice reveals subtile abnormalities in myocardial perfusion and mechanics. Am J Physiol Heart Circ Physiol 290:H2516–H2521

    Article  PubMed  CAS  Google Scholar 

  • Nakashima Y, Plump AS, Raines EW, Breslow JL, Ross R (1994) ApoE-deficient mice develop lesions of all phases of atherosclerosis throughout the arterial tree. Arterioscler Thromb 14: 133–140

    PubMed  CAS  Google Scholar 

  • Oppelt A, Graumann R, Barfuss H, Fischer H, Hartl W, Schajor W (1986) FISP – a new fast MRI sequence. Electromedica 54:15–18

    Google Scholar 

  • Rommel E, Kuhstrebe J, Wiesmann F, Szimtenings M, Streif J, Haase A (2000) A double trigger unit for ECG and breath triggered mouse heart imaging. MAGMA 11:250

    Google Scholar 

  • Rudin M, Pedersen B, Umemura K, Zierhut W (1991) Determination of rat heart morphology and function in vivo in two models of cardiac hypertrophy by means of magnetic resonance imaging. Basic Res Cardiol 86:165–174

    Article  PubMed  CAS  Google Scholar 

  • Ruff J, Wiesmann F, Hiller KH, Neubauer S, Rommel E, Haase A (1998) Influence of isoflurane anesthesia on contractility of mouse heart in vivo. An NMR imaging study. MAGMA 6:169

    Google Scholar 

  • Ruff J, Wiesmann F, Hiller KH, Voll S, von Kienlin M, Bauer WR, Rommel E, Neubauer S, Haase A (1998) Magnetic resonance microimaging for noninvasive quantification of myocardial function and mass in the mouse. Magn Reson Med 40:43–48

    Article  PubMed  CAS  Google Scholar 

  • Saupe KW, Spindler M, Hopkins JC, Shen W, Ingwall JS (2000) Kinetic, thermodynamic, and developmental consequences of deleting creatine kinase isoenzymes from the heart. Reaction kinetics of the creatine kinase isoenzymes in the intact heart. J Biol Chem 275:19742–19746

    Article  PubMed  CAS  Google Scholar 

  • Schwarzbauer C, Morissey S, Haase A (1996) Quantitative magnetic resonance imaging of perfusion using magnetic labeling of water protons within the detection slice. Magn Reson Med 35: 540-546

    PubMed  CAS  Google Scholar 

  • Seo HS, Lombardi DM, Polinsky P, Powell-Braxton L, Bunting S, Schwartz SM, Rosenfeld ME (1997) Peripheral vascular stenosis in apolipoprotein E-deficient mice. Potential roles of lipid deposition, medial atrophy, and adventitial inflammation. Arterioscler Thromb Vasc Biol 17:3593–601

    PubMed  CAS  Google Scholar 

  • Shapiro EP, Rogers WJ, Beyar, R, Soulen RL, Zerhouni EA, Lima JA, Weiss JL (1989) Determination of left ventricular mass by magnetic resonance imaging in hearts deformed by acute infarction. Circulation 79:706–711

    PubMed  CAS  Google Scholar 

  • Siri FM, Jelicks LA, Leinwand LA, Gardin JM (1997) Gated magnetic resonance imaging of normal and hypertrophied murine hearts. Am J Physiol 272:H2394–H2402

    PubMed  CAS  Google Scholar 

  • Slawson SE, Roman BB, Williams DS, Koretsky AP (1998) Cardiac MRI of the normal and hypertrophied mouse heart. Magn Reson Med 39:980–987

    Article  PubMed  CAS  Google Scholar 

  • Streif JUG, Herold V, Szimtenings M, Lanz TE, Nahrendorf M, Wiesmann F, Bauer WR, Rommel E, Haase A (2003) In vivo time-resolved quantitative motion mapping of the murine myocardium with phase contrast MRI. Magn Reson Med 49:315–321

    Article  PubMed  Google Scholar 

  • Streif JUG, Nahrendorf M, Hiller KH, Waller C, Wiesmann F, Rommel E, Haase A, Bauer WR (2005) In vivo assessment of absolute perfusion and intracapillary blood volume in the murine myocardium by spin labeling magnetic resonance imaging. Magn Res Med 53:584–592

    Article  Google Scholar 

  • Waller C, Kahler E, Hiller KH, Hu K, Nahrendorf M, Voll S, Haase A, Ertl G, Bauer WR (2000) Myocardial perfusion and intracapillary blood volume in rats at rest and with coronary dilatation: MR imaging in vivo with use of a spin-labeling technique. Radiology 215:189–197

    PubMed  CAS  Google Scholar 

  • Waller C, Hiller KH, Kahler E, Hu K, Nahrendorf M, Voll S, Haase A, Ertl G, Bauer WR (2001) Serial magnetic resonance imaging of microvascular remodeling in the infarcted rat heart. Circulation 103:1564–1569

    PubMed  CAS  Google Scholar 

  • Wang Y, Rossman PJ, Grimm RC, Riederer SJ, Ehmann RL (1996). Navigator-echo-based real-time respiratory gating and triggering for reduction of respiration effects in three-dimensional coronary MR angiography. Radiology 198:55–60

    PubMed  CAS  Google Scholar 

  • Weiss RG, Chatham JC, Georgakopolous D, Charron MJ, Walliman T, Kay L, Walzel B, Wang Y, Kass DA, Gerstenblith G, Chacko VP (2002) An increase in the myocardial PCr/ATP ratio in GLUT4 null mice. FASEB J 15:1077–1079

    Google Scholar 

  • Wiesmann F, Ruff J, Hiller KH, Rommel E, Haase A, Neubauer S (2000) Developmental changes of cardiac function and mass assessed with MRI in neonatal, juvenil, and adult mice. Am J Physiol Heart Circ Physiol 278:H652–H657

    PubMed  CAS  Google Scholar 

  • Wiesmann F, Ruff J, Dienesch C, Laupold A, Illinger R, Frydrychowicz A, Hiller KH, Rommel E, Haase A, Neubauer S (2001) Dobutamine stress magnetic resonance microimaging in mice: acute changes of cardiac geometry and function in normal and failing murine hearts. Circ Res 88:563–569

    PubMed  CAS  Google Scholar 

  • Wiesmann F, Frydrychowicz A, Rautenberg J, Illinger R, Rommel E, Haase A, Neubauer S (2002) Analysis of right ventricular function in healthy mice and a murine model of heart failure by in vivo MRI. Am J Physiol Heart Circ Physiol 283:H1065–H1071

    PubMed  CAS  Google Scholar 

  • Wiesmann F, Szimtenings M, Frydrychowicz A, Illinger R, Hunecke A, RommelE, Neubauer S, Haase A (2003) High-resolution MRI with cardiac and respiratory gating allows for accurate in vivo atherosclerotic plaque visualization in the murine aortic arch. Magn Reson Med 50:69–74

    Article  PubMed  Google Scholar 

  • Wilke N, Simm C, Zhang J, Ellermann J, Ya X, Merkle H, Path G, Ludemann H, Bache R, Urgurbil K (1993) Contrast-enhanced first pass myocardial perfusion imaging: correlation between myocardial blood flow in dogs at rest and during hyperemia. Magn Res Med 29:485–497

    Article  CAS  Google Scholar 

  • Wood ML, Henkelman RM (1986) The magnetic field dependence of the breathing artifact. Magn Reson Imaging 4:387–392

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Physikalisches Institut, Universität Würzburg, 97074, Würzburg, Germany

    Karl-Heinz Hiller, Axel Haase & Peter M. Jakob

  2. Medizinische Klinik und Poliklinik I/Herzkreislaufzentrum, 97080, Würzburg, Germany

    Christiane Waller

  3. MRB Research Center of Magnetic Resonance Bavaria, 97074, Würzburg, Germany

    Axel Haase & Peter M. Jakob

Authors
  1. Karl-Heinz Hiller
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Christiane Waller
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Axel Haase
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Peter M. Jakob
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Karl-Heinz Hiller .

Editor information

Editors and Affiliations

  1. Forschungsschwerpunkt Innovative Krebsdiagnostik und -therapie (E), Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, 69120, Heidelberg, Germany

    Wolfhard Semmler (Leiter der Abteilung für Medizinische Physik in der Radiologie (E 020) (Leiter der Abteilung für Medizinische Physik in der Radiologie (E 020)

  2. Nuklearmedizinische Klinik und Poliklinik, Klinikum rechts des Isar des Technischen Universität München, Ismaninger Str. 22, 81675, München, Germany

    Markus Schwaiger

Rights and permissions

Reprints and permissions

Copyright information

© 2008 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Hiller, KH., Waller, C., Haase, A., Jakob, P.M. (2008). Magnetic Resonance of Mouse Models of Cardiac Disease. In: Semmler, W., Schwaiger, M. (eds) Molecular Imaging II. Handbook of Experimental Pharmacology, vol 185/2. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-77496-9_10

Download citation

Publish with us

Policies and ethics

Search

Navigation