All members of the sulfotransferase (SOT, EC 2.8.2.-) protein family use 3’-phosphoadenosine 5’-phosphosulfate as sulfuryl donor and transfer the sulfate group to an appropriate hydroxyl group of several classes of substrates. In plants, sulfate conjugation reactions seem to play an important role in plant growth, development, and adaptation to stress. The genome of Arabidopsis thaliana contains in total 21 genes that are likely to encode SOT proteins. The respective genes are differentially expressed under various biotic and abiotic stress conditions. Most of their substrates, and therefore the respective physiological roles, of plant SOT proteins are not known. In Arabidopsis three SOT proteins catalyze the last step of glucosinolate (Gl) biosynthesis. In vitro enzyme assays revealed preferences of the recombinant SOT proteins for chemically different types of Gls. In planta Gls exist side by side; therefore initial results from one-substrate measurements were verifi ed using a mixture of ds-Gls and Gl leaf extracts from Arabidopsis as substrates. These studies confi rmed the in vitro measurements. The putative role of SOT proteins in the manipulation of Gl biosynthesis and regulatory aspects will be discussed.
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Klein, M., Papenbrock, J. (2008). Sulfotransferases and Their Role in Glucosinolate Biosynthesis. In: Khan, N.A., Singh, S., Umar, S. (eds) Sulfur Assimilation and Abiotic Stress in Plants. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-76326-0_7
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