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Cellular Physiology of Epithelium and Endothelium

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Applied Scanning Probe Methods IX

Part of the book series: Nano Science and Technolgy ((NANO))

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Abstract

Epithelial and endothelial cell layers have many essential functions for keeping higher organisms alive. One main task which they both share is the regulation of water balance in the body. Regulation of water permeability can be performed by altering the conductivity between the cells (paracellularly) or through the cells (transcellularly). Here, four examples are shown of how atomic force microscopy (AFM) can indirectly assess the effects of these physiological functions on primary cell preparations under buffer conditions. (1) assessing the tightness of paracellular junctions via their height profile, (2) softening of the cytoskeleton in response to thirst hormone, (3) probing intracellular vacuoles and (4) exploring the invagination formed by a transmigrating immune cell after nanomanipulation. It can be concluded that AFM can help us to examine mechanisms regulating the permeability of cell layers.

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Authors and Affiliations

  1. Institute of Physiology II, University of Münster, Robert-Koch-Straße 27b, 48149, Münster, Germany

    Christoph Riethmüller & Hans Oberleithner

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  1. Christoph Riethmüller
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  2. Hans Oberleithner
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Editor information

Editors and Affiliations

  1. Advanced Institute of Science & Technology, School of Materials Science, 923-1292, Ishikawa, Japan

    Masahiko Tomitori

  2. Nanotribology Laboratory for Information Storage and MEMS/NEMS (NLIM), The Ohio State University, W390 Scott Laboratory, 201W. 19th Avenue, 43210-1142, Columbus, Ohio, USA

    Bharat Bhushan

  3. Institute of Physics, FB 16, University of Münster, Wilhelm-Klemm-Str. 10, 48149, Münster, Germany

    Harald Fuchs

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Riethmüller, C., Oberleithner, H. (2008). Cellular Physiology of Epithelium and Endothelium. In: Tomitori, M., Bhushan, B., Fuchs, H. (eds) Applied Scanning Probe Methods IX. Nano Science and Technolgy. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-74083-4_6

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