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Towards a Nanoscale View of Microbial Surfaces Using the Atomic Force Microscope

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Applied Scanning Probe Methods IX

Part of the book series: Nano Science and Technolgy ((NANO))

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Abstract

In recent years, the atomic force microscope (AFM) has greatly improved our understanding of microbial surfaces. AFM imaging has proved to be a powerful tool for visualizing membrane proteins and live cells at high resolution and in physiological conditions. In addition, AFM force spectroscopy has enabled us to probe and map a variety of properties, including the elasticity of cell walls and cell surface molecules, and the unfolding forces of single proteins, and has allowed the detection and functional analysis of molecular recognition sites. These unique capabilities allow researchers to answer a number of questions that were inaccessible before, such as how does the surface architecture of microbes change as they grow or as they interact with an antibiotic, what are the conformational changes in single membrane proteins, and what are the molecular forces responsible for the interaction between host cells or specific molecules?

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Author information

Authors and Affiliations

  1. Unité de Chimie des Interfaces, Université catholique de Louvain, Croix du Sud 2/18, 1348, Louvain-la-Neuve, Belgium

    Claire Verbelen, Guillaume Andre, Xavier Haulot, Yann Gilbert, David Alsteens, Etienne Dague & Yves F. Dufrene

Authors
  1. Claire Verbelen
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  2. Guillaume Andre
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  3. Xavier Haulot
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  4. Yann Gilbert
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  5. David Alsteens
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  6. Etienne Dague
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  7. Yves F. Dufrene
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Editor information

Editors and Affiliations

  1. Advanced Institute of Science & Technology, School of Materials Science, 923-1292, Ishikawa, Japan

    Masahiko Tomitori

  2. Nanotribology Laboratory for Information Storage and MEMS/NEMS (NLIM), The Ohio State University, W390 Scott Laboratory, 201W. 19th Avenue, 43210-1142, Columbus, Ohio, USA

    Bharat Bhushan

  3. Institute of Physics, FB 16, University of Münster, Wilhelm-Klemm-Str. 10, 48149, Münster, Germany

    Harald Fuchs

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Verbelen, C. et al. (2008). Towards a Nanoscale View of Microbial Surfaces Using the Atomic Force Microscope. In: Tomitori, M., Bhushan, B., Fuchs, H. (eds) Applied Scanning Probe Methods IX. Nano Science and Technolgy. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-74083-4_5

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