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Axenic Culture of Symbiotic Fungus Piriformospora indica

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Plant Surface Microbiology

A large number of media compositions are available in the literature for the cultivation of various groups of fungi, but almost no literature is available for axenic cultivation of symbiotic fungi. In this chapter, we have made efforts to provide the documentary evidence for growth and multiplication of Piriformospora indica (see Chap. 15, this Vol. for characteristic features of the fungus). This new fungus named P. indica, due to its characteristic spore morphology, improves the growth and overall biomass production of different plants, herbs and trees, etc., and can easily be cultivated on a number of complex and synthetic media (Varma et al. 1999, 2001; Singh An et al. 2003a, b). Significant quantitative and morphological changes were detected when the fungus was grown on different nutrient compositions with no apparent negative effect on plants. It is relevant to mention here that different media can be used to understand the morphological and functional properties, or to test possible biotechnological applications.

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References and Selected Reading

  • Ahuja MR (1986) In: Evans DA, Sharp WR and Ammirato PJ (eds) Handbook of plant cell culture 4, techniques and applications. Macmillan, New York, pp 626-651

    Google Scholar 

  • Badoni RJ (1972) Terrestrial occurrence of some aquatic Hyphomycetes. Can J Bot 50:2283-2288

    Article  Google Scholar 

  • Galloway LD, Burgess R (1952) Applied mycology and bacteriology, 3rd edn. Leonard Hill, London, pp 54-57

    Google Scholar 

  • Herrmann S, Munch JC, Buscot F (1998) A gnotobiotic culture system with oak micro-cuttings to study specific effects of mycobionts on plant morphology before, and in the early phase of, ectomycorrhiza formation by Paxillus involutus and Piloderma croceum. New Phytol 138:203-212

    Article  Google Scholar 

  • Johnson CN, Stout PR, Broyer RC, Carlton AB (1957) Comparative chlorine requirements of different plant species. Plant Soil 8:337-353

    Article  CAS  Google Scholar 

  • Kaefer E (1977) Meiotic and mitotic recombination in Aspergillus and its chromosomal aberrations. Adv Genet 19:33-131

    Article  Google Scholar 

  • Kottke I, Guttenberger M, Hampp R, Oberwinkler F (1987) An in vitro method for estab-lishing mycorrhizae on coniferous tree seedlings. Trees 1:191-194

    Article  Google Scholar 

  • Martin JP (1950) Use of acid, rose bengal and streptomycin in the plate method for estimating soil fungi. Soil Sci 69:215-232

    Article  CAS  Google Scholar 

  • Marx, DH (1969) The influence of ectotrophic mycorrhizal fungi on the resistance of pine roots to pathogenic infections. I. Antagonism of mycorrhizal fungi to root path-ogenic fungi and soil bacteria. Phytopathology 59:153-163

    Google Scholar 

  • Moser M (1960) Die Gattung Phlegmacium. J Klinkhardt, Bad Heilbrunn, Austria

    Google Scholar 

  • Mukerji KG, Mandeep, Varma A (1998) Mycorrhizosphere microorganisms: screening and evaluation. In: Varma A (ed) Mycorrhiza manual. Springer, Berlin Heidelberg New York, pp 85-98

    Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol Plant 15:431-487

    Article  Google Scholar 

  • Schüßler A, Schwarzott D, Walker C (2001) A new fungal phylum, the Glomeromycota: phylogeny and evolution. Mycol Res 105:1413-1421

    Article  Google Scholar 

  • Singh An, Singh Ar, Kumari M, Rai MK, Varma A (2003a) Biotechnological importance of Piriformospora indica Verma et al. a novel symbiotic mycorrhiza-like fungus: an overview. Indian J Biotechnol 2:65-75

    Google Scholar 

  • Singh An, Singh Ar, Kumari M, Kumar S, Rai MK, Sharma AP and Varma A (2003b) Unmassing the accessible treasures of the hidden unexplored microbial world. In: Prasad BN (ed) Biotechnology in sustainable biodiversity and food security. Science Publishers, Inc. Enfield, NH, USA, pp 101-124

    Google Scholar 

  • Van der Heijden MAG (2002) Arbuscular mycorrhizal fungi as a determinant of plant diversity: in search for underlying mechanisms and general principles. In: Van der Heijden MGA and Sanders IR (eds) Mycorrhizal ecology. Ecological Studies 157. Springer, Berlin Heidelberg New York, pp 243-266

    Google Scholar 

  • Varma A, Verma S, Sudha, Sahay NS, Franken P (1999) Piriformospora indica, a cultivable plant growth promoting root endophyte with similarities to arbuscular mycorrhizal fungi. Appl Environ Microbiol 65:2741-2744

    CAS  PubMed  Google Scholar 

  • Varma A, Singh A, Sudha, Sahay NS, Sharma J, Roy A, Kumari M, Rana D, Thakran S, Deka D, Bharati K, Hurek T, Blechert O, Rexer KH, Kost G, Hahn A, Hock B, Maier W, Wal-ter M, Strack D, Kranner I (2001) Piriformospora indica: An axenically culturable mycorrhiza-like endosymbiotic fungus. In: Hock B (ed) Mycota IX. Springer, Berlin Heidelberg New York, pp 123-150

    Google Scholar 

  • Varma A, Singh A, Sudha, Sahay NS, Kumari M, Bharti K, Sarbhoy AK, Maier W, Walter MH, Strack D, Franken P, Singh An, Malla R, Hurek T (2002) Piriformospora indica: A plant stimulator and pathogen inhibitor arbuscular mycorrhizal-like fungus. In: Markandey DK, Markandey NR (eds) Microorganisms in bioremediation. Capital Publishing Company Ltd. , New Delhi, pp 71-89

    Google Scholar 

  • Verma S, Varma A, Rexer KH, Hassel A, Kost G, Sarbhoy A, Bisen P, Bütehorn B, Franken P (1998) Piriformospora indica gen. et sp. nov. , a new root-colonizing fungus. Mycolo-gia 90:895-909

    Google Scholar 

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Pham, G.H. et al. (2008). Axenic Culture of Symbiotic Fungus Piriformospora indica . In: Varma, A., Abbott, L., Werner, D., Hampp, R. (eds) Plant Surface Microbiology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-74051-3_30

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  • DOI: https://doi.org/10.1007/978-3-540-74051-3_30

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-74050-6

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