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Mobility and Signaling of Single Receptor Proteins

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Single Molecules and Nanotechnology

Part of the book series: Springer Series in Biophysics ((BIOPHYSICS,volume 12))

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Because many central cellular signaling processes are initiated by receptor mediated detection of single or few physical or molecular stimuli, such processes are ideally suited to be investigated by single-molecule techniques. This review concerns imaging of cellular signaling processes by single molecule microscopies concentrating on cell surface membrane receptors and nuclear receptors. First we give a comprehensive overview on novel developments to label proteins selectively in live cells. Then we discuss in detail how individual membrane proteins labeled with small organic fluorophores, quantum dots, or gold nanoparticles can be imaged in real-time on the upper cell surface with nanometer precision by single-particle tracking, or inside of live cells, by fluorescence correlation spectroscopy at native low expression levels. We report how single-molecule microscopies and spectroscopies can offer substantial new quantitative information to decipher the complex cellular biochemistries with exceptional spatial and temporal resolution.

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Authors and Affiliations

  1. Institute of Chemical Sciences and Engineering, Swiss Federal Institute of Technology (EPFL), CH-1015, Lausanne, Switzerland

    Michael Prummer & Horst Vogel

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  1. Michael Prummer
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  2. Horst Vogel
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Editors and Affiliations

  1. Laboratory of Medical Biophysics, MBB, Karolinska Institute, S-171 77, Stockolm, Sweden

    R. Rigler

  2. Laboratory of Physical Chemistry of Polymers and Membranes Institute of Chemical Sciences and Engineering, Swiss Federal Institute of Technology (EPFL), CH-1015, Lausanne, Switzerland

    H. Vogel

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Prummer, M., Vogel, H. (2008). Mobility and Signaling of Single Receptor Proteins. In: Rigler, R., Vogel, H. (eds) Single Molecules and Nanotechnology. Springer Series in Biophysics, vol 12. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-73924-1_6

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