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Application of Laser-Assisted Microdissection for Tissue and Cell-Specific Analysis of RNA, Proteins, and Metabolites

  • Dirk Hölscher
  • Bernd Schneider
Part of the Progress in Botany book series (BOTANY, volume 69)

The present state of different laser-assisted microdissection methods and their application in various disciplines of plant science is reviewed. The first part of the present review describes the basic effects of a laser beam on biological tissue, and following sections dealing with practical aspects of sample preparation and the technologies implemented in laser-assisted microdissection procedures. An attempt is made to distinguish laser-capture microdissection from laser cutting, to distinguish different varieties of laser cutting, and to discuss the advantages and drawbacks of the various methods for specific applications. The second part of the review covers applications of laser-assisted microdissection in various areas of plant science. Using lasers to conduct microsurgery on plant tissue and to dissect chromosome parts are two highly specialized areas that are discussed. RNA isolation and cell-specific gene expression analysis are the most frequent reasons for using laser-assisted microdissection methods, so a section comprising recent applications is included. Although proteomic methods for analyzing the contents of specific cell populations are available, proteins have been analyzed infrequently in laser-microdissected plant tissue. Therefore, the corresponding section also includes examples from animal samples. The final section is dedicated to the emerging field of cell type-specific metabolite profiling, including secondary metabolites, in extracts of laser-microdissected plant samples by mass spectrometry and nuclear magnetic resonance spectroscopy.

Keywords

Laser Ablation Vascular Bundle Laser Capture Microdissection Laser Cutting Pulse Laser Ablation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Angeles G, Berrio-Sierra J, Joseleau J-P, Lorimier P, Lefèbvre A, Ruel K (2006) Preparative laser capture microdissection and single-pot cell wall material preparation: a novel method for tissue-specific analysis. Planta 224:228–232.PubMedGoogle Scholar
  2. Asano T, Masumura T, Kusano H, Kikuchi S, Kurita A, Shimada H, Kadowaki KI (2002) Construction of a specialized cDNA library from plant cells isolated by laser capture microdissection: toward comprehensive analysis of the genes expressed in the rice phloem. Plant J 32:401–408.PubMedGoogle Scholar
  3. Ball HJ, Hunt NH (2004) Needle in a haystack: microdissecting the proteome of a tissue. Amino Acids 27:1–7.PubMedGoogle Scholar
  4. Bhattacharya SH, Gal AA, Murray KK (2003) Laser capture microdissection MALDI for direct analysis of archival tissue. J Proteome Res 2:95–98.PubMedGoogle Scholar
  5. Boulnois JL (1986) Photophyiscal processes in recent medical laser developments: a review. Lasers Med Sci 1:47–66.Google Scholar
  6. Brandt S (2005) Microgenomics: gene expression analysis at the tissue-specific and single-cell levels. J Exp Bot 56:495–505.PubMedGoogle Scholar
  7. Brandt S, Kehr J, Walz C, Imlau A, Willmitzer L, Fisahn J (1999) A rapid method for detection of plant gene transcripts from single epidermal, mesophyll and companion cell of intacts leaves. Plant J 20:245–250.PubMedGoogle Scholar
  8. Brandt S, Walz C, Schad M, Pavlovic N, Kehr J (2003) A simple, chisel-assisted mechanical microdissection system for harvesting homogenous plant tissue suitable for the analysis of nucleic acids and proteins. Plant Mol Biol Rep 21:417–427.Google Scholar
  9. Brinkmann R, Hansen C, Mohrenstecher D, Scheu M, Birngruber R (1996) Analysis of cavitation dynamics during pulsed laser tissue ablation by optical on-line monitoring. IEEE J Sel Top Quantum Electron 2:826–835.Google Scholar
  10. Burgemeister R (2005) New aspects of laser microdissection in research and routine. J Histochem Cytochem 53:409–412.PubMedGoogle Scholar
  11. Cai S, Lashbrook CC (2006) Laser capture microdissection of plant cells from tape-transferred paraffin sections promotes recovery of structurally intact RNA for global gene profiling. Plant J 48:628–637.PubMedGoogle Scholar
  12. Caldwell RL, Caprioli RM (2005) Tissue profiling by mass spectrometry. Mol Cell Proteomics 4:394–401.PubMedGoogle Scholar
  13. Cantor CR, Schimmel PR (1980) Biophysical chemistry (I). Freeman, New York.Google Scholar
  14. Casson S, Spencer M, Walker K, Lindsey K (2005) Laser capture microdissection for the analysis of gene expression during embryogenesis of Arabidopsis. Plant J 42:111–123.PubMedGoogle Scholar
  15. Corpas FJ, Fernández-Ocaña A, Carreras A, Valderrama R, Luque F, Esteban FJ, Rodríguez-Serrano M, Chaki M, Pedrajas JR, Sandalio LM, Río LA del, Barroso JB (2006) The expression of different superoxide dismutase forms is cell-type dependent in olive (Olea europaea L.) leaves. Plant Cell Physiol 47:984–994.PubMedGoogle Scholar
  16. Cox KH, Goldberg RB (1988) Analysis of gene expression. In: Shaw CH (ed) Plant molecular biology: a practical approach. IRL, Oxford, pp 1–34.Google Scholar
  17. Craven RA, Banks RE (2001) Laser capture microdissection and proteomics: possibilities and limitation. Proteomics 1:1200–1204.PubMedGoogle Scholar
  18. Craven RA, Totty N, Harnden P, Selby PJ, Banks RE (2002) Laser capture microdissection and two-dimensional polyacrylamide gel electrophoresis. Am J Pathol 160:815–822.PubMedGoogle Scholar
  19. Day RC, Grossniklaus U, Macknight RC (2005) Be more specific! Laser-assisted microdissection of plant cells. Trends Plant Sci 10:398–406.Google Scholar
  20. Day RC, McNoeb LA, Macknight RC (2007) Transcript analysis of laser microdissected plant cells. Physiol Plant 129:267–282.Google Scholar
  21. Doukas AG, McAucliffe DJ, Lee S, Venugopalan V, Flotte TJ (1995) Physical factors involved in stress-wave-induced cell injury–the effect of stress gradient. Ultrasound Med Biol 21:961–975.PubMedGoogle Scholar
  22. Elvers D, Remer L, Arnold N, Bäuerle D (2005) Laser microdissection of biological tissues: process optimization. Appl Phys A 80:55–59.Google Scholar
  23. Emmert-Buck MR, Bonner RF, Smith PD, Chuaqui RF, Zhuang Z, Goldstein SR, Weiss RA, Liotta LA (1996) Laser capture microdissection. Science 274:998–1001.PubMedGoogle Scholar
  24. Emmony DC, Geerken BM, Straaijer A (1976) Interaction of 10.6 mm laser radiation with liquids. Infrared Phys 16:87–92.Google Scholar
  25. Frenz M, Konz F, Pratisto H, Weber HP, Silenok AS, Konov VI (1998) Starting mechanisms and dynamics of bubble formation induced by a Ho:Yttrium aluminium garnet laser in water. J Appl Phys 84:5905–5912.Google Scholar
  26. Fukui K, Minezawa M, Kamisugi Y, Ishikawa M, Ohmido N, Yanagisawa T, Fujishita M, Sakai E (1992) Microdissection of plant chromosomes by argon-ion laser beam. Theor Appl Genet (1992) 84:787–791.Google Scholar
  27. Garrison BJ, Srinivasan R (1985) Laser ablation of organic polymers–microscopic models for photochemical and thermal-processes. J Appl Phys 57:2909–2914.Google Scholar
  28. Gitomer SJ, Jones RD (1991) Laser-produced plasmas in medicine. IEEE Trans Plasma Sci 19:1209–1219.Google Scholar
  29. Goldsworthy SM, Stockton PS, Trempus CS, Foley JF, Maronpot RR (1999) Effects of fixation on RNA extraction and amplification from laser capture microdissected tissue. Mol Carcinogen 25:86–91.Google Scholar
  30. Greulich KO, Weber G (1992) The light microscope on its way from an analytical to a preparative tool. J Microsc 167:127–151.Google Scholar
  31. Grubb RL, Calvert VS, Wulkuhle JD, Paweletz CP, Linehan WM, Phillips JL, Chuaqui R, Valasco A, Gillespie J, Emmert-Buck M, Liotta LA, Petricoin EF (2003) Signal pathway profiling of prostate cancer using reverse phase protein arrays. Proteomics 3:2142–2146.PubMedGoogle Scholar
  32. Hobza R, Lengerova M, Cernohorska M, Rubes J, Vyskot B (2004) FAST-FISH with laser beam microdissected DOP-PCR probe distinguishes the sex chromosomes of Silene latifolia. Chromosome Res 12:245–250.PubMedGoogle Scholar
  33. Hölscher D, Schneider B (2007) Laser microdissection and cryogenic nuclear magnetic resonance spectroscopy, an alliance for cell type-specific metabolite profiling. Planta 225:763–770.PubMedGoogle Scholar
  34. Ivashikina N, Deeken R, Ache P, Kranz E, Pommerrenig B, Sauer N, Hedrich R (2003) Isolation of AtSUC2promoter-GFP-marker companion cells for patch-clamp studies and expression profiling. Plant J 36:931–945.PubMedGoogle Scholar
  35. Jiang K, Zhang S, Lee S, Tsai G, Kim K, Huang H, Chilcott C, Zhu T, Feldman LJ (2006) Transcription profile analyses identify genes and pathways central to root cap functions in maize. Plant Mol Biol 60:343–363.PubMedGoogle Scholar
  36. Karrer EE, Lincoln JE, Hogenhout S, Bennett AB, Bostock RM, Martineau B, Lucas WJ, Gilchrist DG, Alexander D (1995) In situ isolation of messenger-RNA from individual plant-cells-creation of cell-specific cDNA libraries. Proc Natl Acad Sci USA 92:3814–3818.PubMedGoogle Scholar
  37. Kawamura Y, Toyoda K, Namba S (1982) Effective deep ultraviolet photoetching of polymethyl methacrylate by an excimer laser. Appl Phys Lett 40:374–375.Google Scholar
  38. Kehr J (2003) Single cell technology. Curr Opin Plant Biol 6:1–5.Google Scholar
  39. Kerk N, Ceserani T, Tausta SL, Sussex IM, Nelson TM (2003) Laser capture microdissection of cells from plant tissues. Plant Physiol 132:27–35.PubMedGoogle Scholar
  40. Kitai MS, Popkov VL, Semchishen VA, Kharizov AA (1991) The physics of uv laser cornea ablation. IEEE J Quantum Electron 27:302–307.Google Scholar
  41. Klink VP, MacDonald M, Alkharouf N, Matthews BF (2005) Laser capture microdissection (LCM) and expression analyses of Glycine max (soybean) syncytium containing root regions formed by the plant pathogen Heterodera glycines (soybean cyst nematode). Plant Mol Biol 59:969–983.Google Scholar
  42. Kovacs H, Moskau D, Spraul M (2005) Cryogenically cooled probes–a leap in NMR technology. Progr Nucl Mag Res Spec 46:131–155.Google Scholar
  43. Krasnov MM (1973) Laseropuncture of anterior chamber angle in glaucoma. Am J Ophthalmol 75:674–678.PubMedGoogle Scholar
  44. Kubo Y, Klimek F, Kikuchi Y, Bannasch P, Hino O (1995) Early detection of knudson two-hits in preneoplastic renal cells of the eker rat model by the laser microdissection procedure. Cancer Res 55:989–990.PubMedGoogle Scholar
  45. Kwapiszewska G, Meyer M, Bogumil R, Bohle RM, Seeger W, Weissmann N, Fink L (2004) Identification of proteins in laser-microdissected small cell numbers by SELDI-TOF and Tandem MS. BMC Biotechnol 4:30.PubMedGoogle Scholar
  46. Lange BM (2005) Single-cell genomics. Curr Opin Plant Biol 8:236–241.PubMedGoogle Scholar
  47. Li S-H, Schneider B, Gershenzon J (2007) Microchemical analysis of laser-microdissected stone cells of Norway spruce by cryogenic nuclear magnetic resonance spectroscopy. Planta 225:771–779.PubMedGoogle Scholar
  48. Liu H, Wang S, Yu X, Yu J, He X, Zhang S, Shou H, Wu P (2005) ARL1, a LOB-domain protein required for adventitious root formation in rice. Plant J 43:47–56.PubMedGoogle Scholar
  49. Lochmann H, Bazzanella A, Bächmann K (1998) Analysis of solutes and metabolites in single plant cell vacuoles by capillary electrophoresis. J Chromatogr 817:337–343.Google Scholar
  50. Lubatschowski H, Heisterkamp A (2004) Interaction with biological tissue. In: Dausinger F, Lichtner F, Lubatschowski H (eds) Topics in applied physics, vol 96. Springer, Berlin Heidelberg New York, pp 91–104.Google Scholar
  51. Maiman TH (1960) Stimulated optical rotation in ruby. Nature 187:493–494.Google Scholar
  52. Matsunaga S, Kawano S, Michimoto T, Higashiyama T, Nakao S, Sakai A, Kuroiwa T (1999a) Semi-automatic laser beam microdissection of the Y chromosome and analysis of Y chromosome DNA in a dioecious plant, Silene latifolia. Plant Cell Physiol 40:60–68.PubMedGoogle Scholar
  53. Matsunaga S, Schütze K, Donnison IS, Grant SR, Kuroiwa T, Kawano S (1999b) Single pollen typing combined with laser-mediated manipulation. Plant J 20:371–378.PubMedGoogle Scholar
  54. Meier-Ruge W, Bielser W, Remy E, Hillenkamp F, Nitsche R, Unsöld R (1976) The laser in the Lowry technique for microdissection of freeze-dried tissue slices. Histochem J 8:387–40l.PubMedGoogle Scholar
  55. Melle C, Ernst G, Schimmel B, Bleul A, Koscielny S, Wiesner A, Bogumil R, Möller U, Osterloh D, Halbhuber KJ, Eggeling F von (2003) Biomarker discovery and identification in laser microdissected head and neck squamous cell carcinoma with ProteinChip technology, two-dimensional gel electrophoresis, tandem mass spectrometry, and immunohistochemistry. Mol Cell Proteomics 2:443–452.PubMedGoogle Scholar
  56. Melle C, Kaufmann R, Hommann M, Bleul A, Driesch D, Ernst G, Eggeling F von (2004) Proteomic profiling in microdissected hepatocellular carcinoma tissue using ProteinChip technology. Int J Oncol 24:885–891.PubMedGoogle Scholar
  57. Monajembashi S, Cremer C, Cremer T, Wolfrum J, Greulich KO (1986) Microdissection of human-chromosomes by a laser microbeam. Exp Cell Res 167:262–265.PubMedGoogle Scholar
  58. Murata J, De Luca V (2005) Localization of tabersonine 16-hydroxylase and 16-OH tabersonine-16-O-methyltransferase to leaf epidermal cells defines them as a major site of precursor biosynthesis in the vindoline pathway in Catharanthus roseus. Plant J 44:581–594.PubMedGoogle Scholar
  59. Nakada M, Komatsu M, Ochiai T, Ohtsu K, Nakazono M, Nishizawa NK, Nitta K, Nishiyama R, Kameya T, Kanno A (2006) Isolation of MaDEF from Muscari armeniacum and analysis of its expression using laser microdissection. Plant Sci 170:143–150.Google Scholar
  60. Nakazono M, Qiu F, Borsuk LA, Schnable PS (2003) Laser-capture microdissection, a tool for the global analysis of gene expression in specific plant cell types: identification of genes expressed differentially in epidermal cells or vascular tissues of maize. Plant Cell 15:583–596.PubMedGoogle Scholar
  61. Nelson T, Tausta SL, Gandotra N, Liu T (2006) Laser microdissection of plant tissue: what you see is what you get. Annu Rev Plant Biol 57:181–201.PubMedGoogle Scholar
  62. Oraevsky AA, Jacques SL, Tittel FK (1995) Mechanism of laser-ablation for aqueous-media irradiated under confined-stress conditions. J Appl Phys 78:1281–1290.Google Scholar
  63. Outlaw WH, Lowry OH (1977) Organic-acid and potassium accumulation in guard cells during stomatal opening. Proc Natl Acad Sci USA 74:4434–4438.PubMedGoogle Scholar
  64. Palmer-Toy DE, Sarracino DA, Sgroi D, LeVangie R, Leopold PE (2000) Direct acquisition of matrix-assisted laser desorption/ionization time-of-flight mass spectra from laser capture microdissected tissues. Clin Chem 46:1513–1516.PubMedGoogle Scholar
  65. Paltauf G, Schmidt-Kloiber H (1995) Model study to investigate the contribution of spallation to pulsed-laser ablation of tissue. Lasers Surg Med 16:277–287.PubMedGoogle Scholar
  66. Paltauf G, Schmidt-Kloiber H (1996). Microcavity dynamics during laser-induced spalltion of liquids and gels. Appl Phys A 62:303–311.Google Scholar
  67. Paweletz CP, Charboneau L, Bichsel VE, Simone NL, Chen T, Gillespie JW, Emmert-Buck MR, Roth MJ, Petricoin EF, Liotta LA (2001) Reverse phase protein microarrays which capture disease progression show activation of pro-survival pathways at the cancer invasion front. Oncogene 20:1981–1989.PubMedGoogle Scholar
  68. Petry R, Schmitt M, Popp J (2003) Raman spectroscopy–a prospective tool in the life sciences. ChemPhysChem 4:14–30.PubMedGoogle Scholar
  69. Polster J, Dithmar H, Burgemeister R, Friedemann G, Feucht W (2006) Flavonoids in plant nuclei: detection by laser microdissection and pressure catapulting (LMPC), in vivo staining, and uv–visible spectroscopic titration. Physiol Plant 128:163–174.Google Scholar
  70. Pommerrenig B, Barth I, Niedermeier M, Kopp S, Schmid J, Dwyer RA, McNair RJ, Klebl F, Sauer N (2006) Common plantain. A collection of expressed sequence tags from vascular tissue and a simple and efficient transformation method. Plant Physiol 142:1427–1441.PubMedGoogle Scholar
  71. Pühler A, Regitz M, Schmid RD (eds) (2000) Römpp Lexikon Biochemie und Molekularbiologie. Thieme, Stuttgart.Google Scholar
  72. Ramsay K, Wang Z, Jones MGK (2004) Using laser capture microdissection to study gene expression in early stages of giant cells induced by root-knot nematodes. Mol Plant Pathol 5:587–592.Google Scholar
  73. Ramsay K, Jones MGK, Wang Z (2006) Laser capture microdissection: a novel approach to microanalysis of plant–microbe interactions. Mol Plant Pathol 7:429–435.Google Scholar
  74. Reinhardt D, Frenz M, Mandel T, Kuhlemeier C (2003) Microsurgical and laser ablation analysis of interactions between the zones and layers of the tomato shoot apical meristem. Development 130:4073–4083.PubMedGoogle Scholar
  75. Reinhardt D, Frenz M, Mandel T, Kuhlemeier C (2005) Microsurgical and laser ablation analysis of leaf positioning and dorsoventral patterning in tomato. Development 132:15–26.PubMedGoogle Scholar
  76. Sanders PM, Bui AQ, Le BH, Goldberg RB (2005) Differentiation and degeneration of cells that play a major role in tobacco anther dehiscence. Sex Plant Reprod 17:219–241.Google Scholar
  77. Scalenghe F, Turco E, Edstrom JE, Pirrotta V, Melli M (1981) Micro-dissection and cloning of DNA from a specific region of Drosophila melanogaster polytene chromosomes. Chromosoma 82:205–216.PubMedGoogle Scholar
  78. Schad M, Lipton MS, Giavalisco P, Smith RD, Kehr J (2005a) Evaluation of two dimensional electrophoresis and liquid chromatography–tandem mass spectrometry for tissue-specific protein profiling of laser-microdissected plant samples. Electrophoresis 26:2729–2738.PubMedGoogle Scholar
  79. Schad M, Mungur R, Fiehn O, Kehr J (2005b) Metabolic profiling of laser microdissected vascular bundles of Arabidopsis thaliana. Plant Methods 1:2.PubMedGoogle Scholar
  80. Schmidt H, Ihlemann J, Wolff-Rottke B, Luther K (1998) Ultraviolet laser ablation of polymers: spot size, pulse duration, and plume attenuation effects explained. J Appl Phys 83, 5458–5468.Google Scholar
  81. Schütze K, Lahr G (1998) Identification of expressed genes by laser-mediated manipulation of single cells. Nat Biotechnol 16:737–742.PubMedGoogle Scholar
  82. Scutt CP, Kamisugi Y, Sakai F, Gilmartin PM (1997) Laser isolation of plant sex chromosomes: studies on the DNA composition of the X and Y sex chromosomes of Silene latifolia. Genome 40:705–715.PubMedGoogle Scholar
  83. Shekouh AR, Thompson CC, Prime W, Campbell F, Hamlett J, Herrington CS, Lemoine NR, Crnogorac-Jurcevic T, Buechler MW, Friess H, Neoptolemos JP, Pennington SR, Simone NL, Remaley AT, Charboneau L, Petricoin EF III, Glickman JW, Emmert-Buck MR, Fleisher TA, Liotta LA (2000) Sensitive immunoassay of tissue cell proteins procured by laser capture microdissection. Am J Pathol 156:445–452.Google Scholar
  84. Snow M, Snow R (1931) Experiments on phyllotaxis. I. The effect of isolating a primordium. Philos Trans R Soc Lond B Biol Sci 221:1–43.Google Scholar
  85. Solon AR, Aronson R, Gould G (1961) Physiological implications of laser beams. Science 134:1506–1508.PubMedGoogle Scholar
  86. Srinivasan R (1986) Ablation of polymers and biological tissue by ultraviolet-lasers. Science 234:559–565.PubMedGoogle Scholar
  87. Srinivasan R, Leigh W (1982) Ablative photodecomposition: action of far-ultraviolet laser radiation (193 nm) on poly(ethylene terephthalate) films. J Am Chem Soc 104:6784–6785.Google Scholar
  88. Srinivasan R, Mayne-Banton V (1982) Self developing photoetching of poly(ethylene-terephthalate) films by far ultraviolet excimer laser-radiation. Appl Phys Lett 41:576–578.Google Scholar
  89. Tomos AD, Sharrock RA (2001) Cell sampling and analysis (SiCSA): metabolites measured at single cell resolution. J Exp Bot 52:623–630.PubMedGoogle Scholar
  90. Tsuji H, Aya K, Ueguchi-Tanaka M, Shimada Y, Nakazono M, Watanabe R, Nishizawa NK, Gomi K, Shimada A, Kitano H, Ashikari M, Matsuoka M (2006) GAMYB controls different sets of genes and its differentially regulated by microRNA in aleurone cells and anthers. Plant J 47:427–444.PubMedGoogle Scholar
  91. Ventzek PLG, Gilgenbach RM, Ching CH, Lindley RA (1992) Schlieren and dye-laser resonance-absorption photographic investigations of KrF excimer laser-ablated atoms and molecules from polyimide, polyethylenterephthalate, and aluminium. J Appl Phys 72:1696–1706.Google Scholar
  92. Vogel A, Venugopalan V (2003) Mechanisms of pulsed laser ablation of biological tissues. Chem Rev 103:577–644.PubMedGoogle Scholar
  93. Vogel A, Busch S, Parlitz U (1996) Shock wave emission and cavitation bubble generation by picosecond and anaosecond optical breakdown in water. J Acoust Soc Am 100:148–165.Google Scholar
  94. Vogel A, Noack J, Nahen K, Theisen D, Busch S, Parlitz U, Hammer DX, Noojin GD, Rockwell BA, Birngruber R (1999) Energy balance of optical breakdown in water at nanosecond to femtosecond time scales. Appl Phys B 68:271–280.Google Scholar
  95. Vogel A, Noack J, Huttman G, Paltauf G (2005) Mechanisms of femtosecond laser nanosurgery of cells and tissues. Appl Phys B 81:1015–1047.Google Scholar
  96. Walsh JT, Deutsch TF (1991) Measurement of Er-YAG laser ablation plume dynamics. Appl Phys B 52:217–224.Google Scholar
  97. Woll K, Borsuk LA, Stransky H, Nettleton D, Schnable PS, Hochholdinger F (2005) Isolation, characterization, and pericycle-specific transcriptome analyses of the novel maize lateral and seminal root initiation mutant rum1. Plant Physiol 139:1255–1267.PubMedGoogle Scholar
  98. Wu S-L, Hancock WS, Goodrich GG, Kunitake ST (2003) An approach to the proteomic analysis of a breast cancer cell line (SKBR-3). Proteomics 3:1037–1046.PubMedGoogle Scholar
  99. Wu YR, Machado AC, White RG, Llewellyn DJ, Dennis ES (2006) Expression profiling identifies genes expressed early during lint fibre initiation in cotton. Plant Cell Physiol 47:107–127.PubMedGoogle Scholar
  100. Xu BJ, Caprioli RM, Sanders ME, Jensen RA (2002) Direct analysis of laser capture microdissected cells by MALDI mass spectrometry. J Am Soc Mass Spectrom 13:1292–1297.PubMedGoogle Scholar
  101. Zang L, Toy DP, Hancock WS, Sgroi DC, Karger BL, J. (2004) Proteomic analysis of ductal carcinoma of the breast using laser capture microdissection, LC-MS, and O-16/O-18 isotopic labeling. J Proteome Res 3:604–612.PubMedGoogle Scholar
  102. Zhu XL, Shaw PN, Pritchard J, Newbury J, Hunt EJ, Barrett DA (2005) Amino acid analysis by micellar electrokinetic chromatography with laser-induced fluorescence detection: Application to nanolitre-volume biological samples from Arabidopsis thaliana and Myzus persicae. Electrophoresis 26:911–919.Google Scholar
  103. Zweig AD (1991) A thermomechanical model for laser ablation. J Appl Phys 70:1684–1691.Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2008

Authors and Affiliations

  • Dirk Hölscher
    • 1
  • Bernd Schneider
    • 1
  1. 1.Max-Planck-Institute for Chemical EcologyBeutenberg CampusJenaGermany

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