The Application of Novel Optical Sensors (Optodes) in Experimental Plant Ecology

Progress and Perspectives in Non-Invasive Bioprocess Analysis and Biogeochemical Interaction
  • Dirk Gansert
  • Stephan Blossfeld
Part of the Progress in Botany book series (BOTANY, volume 69)

The state of the art of optical chemical sensors, optodes, as an enabling technology for non- and minimal invasive bioprocess analysis in plant ecological research is presented. The measuring principle of optodes is briefly described and discussed in respect of their performance, physical properties and analytical efficiency for parameters, such as O2, CO2, temperature or pH, used as measures of bioprocesses. Independence of measurement from the state of aggregation is a major technical a dvantage of optodes that overcomes present limitations of conventional optical sensor techniques. Novel hybrid optodes that provide simultaneous information of two parameters, such as O2 and pH, at the same spot are introduced. A survey of the application of optodes for in vivo bioprocess analysis during the past decade is presented, particularly the use of oxygen optodes in plant ecophysiological research.


Optical Sensor Plant Cell Environ Radial Oxygen Loss Stem Respiration Optical Chemical Sensor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. Absalan G, Soleimani M, Asadi M, Ahmadi MB (2004) Construction of a new optical sensor for monitoring ammonia in water samples using bis (acetylacetoneethylenediamine)-tributylphosphin cobalt(III) tetraphenylborate complex-coated triacetylcellulose. Anal Sci 20:1433–1436.PubMedGoogle Scholar
  2. Alves G, Ameglio T, Guilliot A, Fleurat-Lessard P, Lacointe A, Sakr S, Petel G, Julien J-L (2004) Winter variation in xylem sap pH of walnut trees: involvement of plasma membrane H+-ATPase of vessel-associated cells. Tree Physiol 24:99–105.PubMedGoogle Scholar
  3. Amini MK, Momeni-Isfahani T, Khorasani JH, Pourhossein M (2004) Development of an optical chemical sensor based on 2-(5-bromo-2-pyridylazo)-5-(diethylamino) phenol in Nafion for determination of nickel ion. Talanta 63:713–720.PubMedGoogle Scholar
  4. Arain S, Weiss S, Heinzle E, John GT, Krause C, Klimant I (2005) Gas sensing in microplates with optodes: influence of oxygen exchange between sample, air, and plate material. Biotechnol Bioeng 90:271–280.PubMedGoogle Scholar
  5. Arain S, John GT, Krause C, Gerlach J, Wolfbeis OS, Klimant I (2006) Characterization of microtiterplates with integrated optical sensors for oxygen and pH, and their applications to enzyme activity screening, respirometry, and toxicological assays. Sensor Actuat B Chem 113:639–648.Google Scholar
  6. Armstrong W, Brändle R, Jackson MB (1994) Mechanisms of flood tolerance in plants. Acta Bot Neerl 43:307–358.Google Scholar
  7. Armstrong J, Armstrong W, Beckett PM, Halder JE, Lythe S, Holt R, Sinclair A (1996) Pathways of aeration and the mechanisms and beneficial effects of humidity- and Venturi-induced convections in Phragmites australis (Cav.) Trin. Ex Steud. Aquat Bot 54:177–197.Google Scholar
  8. Armstrong W, Cousins D, Armstrong J, Turner DW, Beckett PM (2000) Oxygen distribution in wetland plant roots and permeability barriers to gas-exchange with the rhizosphere: a microelectrode and modelling study with Phragmites australis. Ann Bot Lond 86:687–703.Google Scholar
  9. Aylott JW (2003) Optical nanosensors–an enabling technology for intracellular measurements. Analyst 128:309–312.PubMedGoogle Scholar
  10. Bailey IW (1913) The preservation treatment of wood. II. The structure of the pit membranes in the tracheids of conifers and their relation to the penetration of gases, liquids, and finely divided solids into green and seasoned wood. Forest Q 11:12–20.Google Scholar
  11. Barclay AM, Crawford RMM (1982) Plant growth and survival under strict anaerobiosis. J Exp Bot 33:541–549.Google Scholar
  12. Bassnett S, McNulty R (2003) The effect of elevated intraocular oxygen on organelle degradation in embryonic chicken lens. J Exp Biol 206:4353–4361.PubMedGoogle Scholar
  13. Bezbaruah AN, Zhang TC (2004) pH, redox, and oxygen microprofiles in the rhizosphere of bulrush (Scirpus validus) in a constructed wetland treating municiple wastewater. Biotechnol Bioeng 88:60–70.PubMedGoogle Scholar
  14. Bloom AJ, Meyerhoff PA, Taylor AR, Rost TL (2003) Root development and absorption of ammonium and nitrate from the rhizosphere. J Plant Growth Regul 21:416–431.Google Scholar
  15. Blossfeld S, Gansert D (2007) A novel non-invasive optical method for quantitative visualization of pH dynamics in the rhizosphere of plants. Plant Cell Environ 30:176–186.PubMedGoogle Scholar
  16. Bonkowski M (2004) Protozoa and plant growth: the microbial loop in soil revisited. New Phytol 162:617–631.Google Scholar
  17. Booth MS, Stark JM, Rastetter E (2005) Controls on nitrogen cycling in terrestrial ecosystems: a synthetic analysis of literature data. Ecol Monogr 75:139–157.Google Scholar
  18. Borisov SM, Vasylevska AS, Krause Ch, Wolfbeis OS (2006a) Composite luminescent material for dual sensing of oxygen and temperature. Adv Funct Mater 16:1536–1542.Google Scholar
  19. Borisov SM, Neurauter G, Schroeder C, Klimant I, Wolfbeis OS (2006b) A modified dual lifetime referencing method for simultaneous optical determination and sensing of two analytes. Appl Spectrosc 60:1167–1173.PubMedGoogle Scholar
  20. Bosc A, Grandcourt A de, Loustau D (2003) Variability of stem and branch maintenance respiration in a Pinus pinaster tree. Tree Physiol 23:227–236.PubMedGoogle Scholar
  21. Bowman WP, Barbour MM, Turnbull MH, Tissue DT, Whitehead D, Griffin KL (2005) Sap flow rates and sapwood density are critical factors in within- and between-tree variation in CO2 efflux from stems of mature Dacrydium cupressinum trees. New Phytol 167:815–828.PubMedGoogle Scholar
  22. Buerk DG (2004) Measuring tissue pO2 with microelectrodes. Methods Enzymol 38:665–690.Google Scholar
  23. Busch J (2001) Characteristic values of key ecophysiological parameters in the genus Carex. Flora 196:1–26.Google Scholar
  24. Buscot F, Munch JC, Charcosset JY, Gardes M, Nehls U, Hampp R (2000) Recent advances in exploring physiology and biodiversity of ectomycorrhizas highlight the functioning of these symbioses in ecosystems. FEMS Miocrobiol Rev 24:601–614.Google Scholar
  25. Bushong FW (1907) Composition of gas from cottonwood trees. Kans Acad Sci Trans 21:53.Google Scholar
  26. Butler JN (1991) Carbon dioxide equilibria and their applications. Lewis, Chelsea.Google Scholar
  27. Campbell A, Uttamchandani D (2004) Optical dissolved oxygen lifetime sensor based on sol–gel immobilisation. IEE Proc Sci Meas Technol 151:291–297.Google Scholar
  28. Chapman SK, Langley JA, Hart SC, Koch GW (2006) Plants actively control nitrogen cycling: uncorking the microbial bottleneck. New Phytol 169:27–34.PubMedGoogle Scholar
  29. Chase WW (1934) The composition, quantity, and physiological significance of gases in tree stems. (Minn Agric Exp Stn Tech Bull 99) University of Minnesota, Minneapolis.Google Scholar
  30. Colmer TD (2003) Long-distance transport of gases in plants: a perspective on internal aeration and radial oxygen loss from roots. Plant Cell Environ 26:17–36.Google Scholar
  31. Colmer TD, Bloom (1998) A comparison of NH4 + and NO3 net fluxes along roots of rice and maize. Plant Cell Environ 21:240–246.Google Scholar
  32. Crawford RMM (1992) Oxygen availability as an ecological limit to plant distribution. Adv Ecol Res 23:93–185.Google Scholar
  33. Crawford RMM (2003) Seasonal differences in plant responses to flooding and anoxia. Can J Bot 81:1224–1246.Google Scholar
  34. Crawford RMM, Braendle R (1996) Oxygen deprivation stress in a changing environment. J Exp Bot 47:145–159.Google Scholar
  35. Davenport J, Irwin S (2003) Hypoxic life of intertidal acorn barnacles. Mar Biol 143:555–563.Google Scholar
  36. Del Hierro AM, Kronberger W, Hietz P, Offenthaler I, Richter H (2002) A new method to determine the oxygen concentration inside the sapwood of trees. J Exp Bot 53:559–563.PubMedGoogle Scholar
  37. Edwards NT, Hanson PJ (1996) Stem respiration in a closed-canopy upland oak forest. Tree Physiol 16:433–439.PubMedGoogle Scholar
  38. Eklund L (1990) Endogenous levels of oxygen, carbon dioxide and ethylene in stems of Norway spruce trees during one growing season. Trees 4:150–154.Google Scholar
  39. Eklund L (1993) Seasonal variations of O2, CO2, and ethylene in oak and maple stems. Can J For Res 23:2608–2610.Google Scholar
  40. Fitter AH, Gilligan CA, Hollingworth K, Kleczkowski A, Twyman RM, Pitchford JW (2005) Biodiversity and ecosystem function in soil. Funct Ecol 19:369–377.Google Scholar
  41. Franke U, Polerecky L, Precht E, Huettel M (2006) Wave tank study of particulate organic matter degradation in permeable sediments. Limnol Oceanogr 51:1084–1096.Google Scholar
  42. Frederiksen MS, Glud RN (2006) Oxygen dynamics in the rhizosphere of Zostera marina: a two-dimensional planar optode study. Limnol Oceanogr 51:1072–1083.Google Scholar
  43. Gansert D (2003) Xylem sap flow as a major pathway for oxygen supply to the sapwood of birch (Betula pubescens Ehr.). Plant Cell Environ 26:1803–1814.Google Scholar
  44. Gansert D (2006) Miniaturisierte und multifunktionale optosensorische Meß- und Regelschleuse für die minimalinvasive Applikation von Mikrosensoren und Mikrowerkzeugen in lebenden und abiotischen Systemen zur quantitativen räumlich und zeitlich hochauflösenden Prozessanalyse, Präparation und experimentellen Manipulation unter Freiland- und Laborbedingungen. Patent DE 102005 018845 A1.Google Scholar
  45. Gansert D, Burgdorf M (2005) Effects of xylem sap flow on carbon dioxide efflux from stems of birch (Betula pendula Roth). Flora 200:444–445.Google Scholar
  46. Gansert D, Burgdorf M, Lösch R (2001) A novel approach to the in situ measurement of oxygen concentrations in the sapwood of woody plants. Plant Cell Environ 24:1055–1064.Google Scholar
  47. Gansert D, Arnold M, Borisov S, Krause C, Müller A, Stangelmayer A, Wolfbeis OS (2006) Hybrid optodes (HYBOP). In: Popp J, Strehle M (eds) Biophotonics: visions for better health care. Wiley–VCH, Weinheim, pp 477–518.Google Scholar
  48. Gatti S, Brey T, Muller WEG, Heilmayer O, Holst G (2002) Oxygen microoptodes: a new tool for oxygen measurements in aquatic animal ecology. Mar Biol 140:1075–1085.Google Scholar
  49. Glud RN, Wenzhofer F, Tengberg A, Middelboe M, Oguri K, Kitazato H (2005) Distribution of oxygen in surface sediments from central Sagami Bay, Japan: in situ measurements by microelectrodes and planar optodes. Deep Sea Res Part I Oceanogr Res Pap 52:1974–1987.Google Scholar
  50. Gollan T, Schurr U, Schulze E-D (1992) Stomatal response to drying soil in relation to changes in the xylem sap composition of Helianthus annuus. I. The concentration of cations, anions amino acids in, and pH of the xylem sap. Plant Cell Environ 15:551–559.Google Scholar
  51. Haberlandt G (1914) Physiological plant anatomy. Macmillan, London.Google Scholar
  52. Hanstein S, Beer D de, Felle HH (2001) Miniaturised carbon dioxide sensor designed for measurements within plant leaves. Sensor Actuat B Chem 81:107–114.Google Scholar
  53. Hazneci C, Ertekin K, Yenigul B, Cetinkaya E (2004) Optical pH sensor based on spectral response of newly synthesized Schiff bases. Dyes Pigments 62:35–41.Google Scholar
  54. Heizmann U, Kreuzwieser J, Schnitzler JP, Brüggemann N, Rennenberg H (2001) Assimilate transport in the xylem sap of pedunculate oak (Quercus robur) saplings. Plant Biol 3:132–138.Google Scholar
  55. Helfter C, Shephard JD, Martinez-Vilalta J, Mencuccini M, Hand DP (2007) A noninvasive optical system for the measurement of xylem and phloem sap flow in woody plants of small stem size. Tree Physiol 27:169–179.PubMedGoogle Scholar
  56. Hinsinger P, Gobran GR, Gregory PJ, Wenzel WW (2005) Rhizosphere geometry and heterogeneity arising from root-mediated physical and chemical processes. New Phytol 168:293–303.PubMedGoogle Scholar
  57. Holst G, Glud RN, Kühl M, Klimant I (1997) A microoptode array for fine-scale measurement of oxygen distribution. Sensor Actuat B Chem:122–129.Google Scholar
  58. Hook DD, Brown CL, Wetmore RH (1972) Aeration in trees. Bot Gaz 133:443–454.Google Scholar
  59. Huber C, Klimant I, Krause C, Wolfbeis OS (2001) Dual lifetime referencing as applied to a chloride optical sensor. Anal Chem 73:2097–2103.PubMedGoogle Scholar
  60. Hulth S, Aller RC, Engstrom P, Selander E (2002) A pH plate fluorosensor (optode) for early diagenetic studies of marine sediments. Limnol Oceanogr 47:212–220.CrossRefGoogle Scholar
  61. Irwin S, Davenport J (2006) Implications of water flow and oxygen gradients for molluscan oxygen uptake and respirometric measurements. J Mar Biol Assoc UK 86:401–402.Google Scholar
  62. Jensen SI, Kuhl M, Glud RN, Jorgensen LB, Prieme A (2005) Oxic microzones and radial oxygen loss from roots of Zostera marina. Mar Ecol Prog Ser 293:49–58.Google Scholar
  63. Kirk G (2005) The biogeochemistry of submerged soils. Wiley, Chichester.Google Scholar
  64. Klimant I, Meyer V, Kühl M (1995) Fiber-optic oxygen microsensors, a new tool in aquatic biology. Limnol Oceanogr 40:1159–1165.CrossRefGoogle Scholar
  65. Klimant I, Kühl M, Glud RN, Holst G (1997) Optical measurement of oxygen and temperature in microscale: strategies and biological applications. Sensor Actuat B Chem 38:29–37.Google Scholar
  66. Klimant I, Huber C, Liebsch G, Neurauter G, Stangelmayer A, Wolfbeis OS (2001) Dual lifetime referencing (DLR)–a new scheme for converting fluorescence intensity into a frequency-domain or time-domain information. In: Valeur B, Brochon JC (eds) New trends in fluorescence spectroscopy: application to chemical and life sciences. Springer, Berlin Heidelberg New York, pp 257–275.Google Scholar
  67. König B, Kohls O, Holst G, Glud RN, Kuhl M (2005) Fabrication and test of sol–gel based planar oxygen optodes for use in aquatic sediments. Mar Chem 97:262–276.Google Scholar
  68. Kosch U, Klimant I, Wolfbeis OS (1999) Long-lifetime based pH micro-optodes without oxygen interference. Fresen J Anal Chem 364:48–53.Google Scholar
  69. Kovacich RP, Martin NA, Clift MG, Stocks C, Gaskin I (2006) Highly accurate measurement of oxygen using a paramagnetic gas sensor. Meas Sci Technol 17:1579–1585.Google Scholar
  70. Lange OL (2002) Photosynthetic productivity of the epilithic lichen Lecanora muralis: long-term field monitoring of CO2 exchange and its physiological interpretation. I. Dependence of photosynthesis on water content, light, temperature, and CO2 concentration from laboratory measurements. Flora 197:1–17.Google Scholar
  71. Lavigne MB, Franklin SE, Hunt ER (1996) Estimating stem maintenance respiration rates of dissimilar balsam fir stands. Tree Physiol 16:687–696.PubMedGoogle Scholar
  72. Lavigne MB, Little CHA, Riding RT (2004) Changes in stem respiration rate during cambial reactivation can be used to refine estimates of growth and maintenance respiration. New Phytol 162:81–93.Google Scholar
  73. Levy PE, Meir P, Allen SJ, Jarvis PG (1999) The effect of aqueous transport of CO2 in xylem sap on gas exchange. Tree Physiol 19:53–58.PubMedGoogle Scholar
  74. Li CY, Zhang XB, Han ZX, Akermark B, Sun LC, Shen GL, Yu RQ (2006) A wide pH range optical sensing system based on a sol–gel encapsulated amino-functionalised corrole. Analyst 131:388–393.PubMedGoogle Scholar
  75. Liebsch G, Klimant I, Wolfbeis OS (1999) Luminescence lifetime temperature sensing based on sol–gels and poly(acrylonitrile) s dyed with ruthenium metal-ligand complexes. Adv Mater 11:1296.Google Scholar
  76. Liebsch G, Klimant I, Krause C, Wolfbeis OS (2001) Fluorescent imaging of pH with optical sensors using time domain dual lifetime referencing. Anal Chem 73:4354–4363.PubMedGoogle Scholar
  77. Lösch R (2001) Wasserhaushalt der Pflanzen. Quelle & Meyer, Wiebelsheim.Google Scholar
  78. Lösch R, Larcher W (1998) Entwicklung und Trends der Ökophysiologie im 20. Jahrhundert. Wetter Leben 50:291–336.Google Scholar
  79. Maberly SC, Madsen TV (1998) Affinity for CO2 in relation to the ability of freshwater macrophytes to use HCO3 . Funct Ecol 12:99–106.Google Scholar
  80. MacDougal DT, Working EB (1933) The pneumatic system of plants, especially trees. (Publication 441) Carnegie Institute of Washington, Washington, D.C.Google Scholar
  81. Maier CA, Clinton BD (2006) Relationship between stem CO2 efflux, stem sap velocity and xylem CO2 concentration in young loblolly pine trees. Plant Cell Environ 29:1471–1483.PubMedGoogle Scholar
  82. Mainiero R, Kazda M (2005) Effects of Carex rostrata on soil oxygen in relation to soil moisture. Plant Soil 270:311–320.Google Scholar
  83. Mancuso S, Marras AM (2003) Different pathways of the oxygen supply in the sapwood of young Olea europaea trees. Planta 216:1028–1033.PubMedGoogle Scholar
  84. Marschner H (1995) Mineral nutrition of higher plants. Academic, London.Google Scholar
  85. Martin TA, Teskey RO, Dougherty PM (1994) Movement of respiratory CO2 in stems of loblolly pine (Pinus taeda L.) seedlings. Tree Physiol 14:481–495.PubMedGoogle Scholar
  86. McGuire MA, Teskey RO (2002) Microelectrode technique for in situ measurement of carbon dioxide concentrations in xylem sap of trees. Tree Physiol 22:807–811.PubMedGoogle Scholar
  87. McKenzie DJ, Wong S, Randall DJ, Egginton S, Taylor EW, Farrell AP (2004) The effects of sustained exercise and hypoxia upon oxygen tensions in the red muscle of rainbow trout. J Exp Biol 207:3629–3637.PubMedGoogle Scholar
  88. Meng XM, Liu L, Guo QX (2005) Progress in fluorescence sensors for Pb(II), Hg(II) and Cd(II) cations. Prog Chem 17:45–54.Google Scholar
  89. Menzel M, Soukup J, Henze D, Engelbrecht K, Senderreck M, Scharf A, Rieger A, Grond S (2003) Experiences with continuous intra-arterial blood gas monitoring: precision and drift of a pure optode-system. Intensive Care Med 29:2180–2186.PubMedGoogle Scholar
  90. Mock T, Dieckmann GS, Haas C, Krell A, Tison JL, Belem AL, Papadimitriou S, Thomas DN (2002) Micro-optodes in sea ice: a new approach to investigate oxygen dynamics during sea ice formation. Aquat Microb Ecol 29:297–306.Google Scholar
  91. Mock T, Kruse M, Dieckmann GS (2003) A new microcosm to investigate oxygen dynamics at the sea ice water interface. Aquat Microb Ecol 30:197–205.Google Scholar
  92. Nagel KA, Schurr U, Walter A (2006) Dynamics of root growth stimulation in Nicotiana tabacum in increasing light intensity. Plant Cell Environ 29:1936–1945.PubMedGoogle Scholar
  93. Negisi K (1979) Bark respiration rate in stem segments detached from young Pinus densiflora trees in relation to velocity of artificial sap flow. J Jpn For Soc 61:88–93.Google Scholar
  94. Neurauter G, Klimant I, Wolfbeis OS (2000) Fiber-optic microsensor for high resolution pCO2 sensing in marine environment. Fresen J Anal Chem 366:481–487.Google Scholar
  95. Nwaigwe CI, Roche MA, Grinberg O, Dunn JF (2003) Brain tissue and sagittal sinus pO2 measurement using the lifetimes of oxygen-quenched luminescence of a ruthenium compound. Oxygen transport to tissue XXIV Adv Exp Med Biol 530:101–111.Google Scholar
  96. Patra AK, Abbadie L, Clays-Josserand A, Degrange V, Grayston SJ, Loiseau P, Louault F, Mahmood S, Nazaret S, Philippot L, Poly F, Prosser JI, Richaume A, Le Raux X (2005) Effects of grazing on microbial functional groups involved in soil N dynamics. Ecol Monogr 75:65–80.Google Scholar
  97. Pearce FJ, Waasdorp C, Hufnagel H, Burtris D, DeFeo J, Soballe P, Drucker WR (2005) Subcutaneous pO2 as an index of the physiological limits for hemodilution in the rat. J Appl Physiol 99:814–821.PubMedGoogle Scholar
  98. Pimenta AM, Araujo AN, Conceicao M, Montenegro BSM, Pasquini C, Rohwedder JJR, Raimundoo IM (2004) Chloride-selective membrane electrodes and optodes based on an indium(III) porphyrin for the determination of chloride in a sequential injection analysis system. J Pharmaceut Biomed 36:49–55.Google Scholar
  99. Polerecky L, Franke U, Werner U, Grunwald B, Beer D de (2005) High spatial resolution measurement of oxygen consumption rates in permeable sediments. Limnol Oceanogr Methods 3:75–85.Google Scholar
  100. Precht E, Franke U, Polerecky L, Huettel M (2004) Oxygen dynamics in permeable sediments with wave-driven pore water exchange. Limnol Oceanogr 49:693–705.CrossRefGoogle Scholar
  101. Pruyn ML, Gartner BL, Harmon ME (2002a) Within-stem variation of respiration in Pseudotsuga menziesii (Douglas-fir) trees. New Phytol 154:359–372.Google Scholar
  102. Pruyn ML, Gartner BL, Harmon ME (2002b) Respiratory potential in sapwood of old versus young ponderosa pine trees in the Pacific Northwest. Tree Physiol 22:105–116.PubMedGoogle Scholar
  103. Radu A, Bakker E (2005) Shifting the measuring range of chloride selective electrodes and optodes based on the anticrown ionophore [9]mercuracarborand-3 by the addition of 1-decanethiol. Chem Anal Warsaw 50:71–83.Google Scholar
  104. Roche P, Al-Jowder R, Narayanaswamy R, Young J, Scully P (2006) A novel luminescent lifetime-based optrode for the detection of gaseous and dissolved oxygen utilising a mixed ormosil matrix containing ruthenium (4, 7-diphenly-1, 10-phenanthroline) (3) Cl-2 (Ru.dpp). Anal Bioanal Chem 386:1245–1257.PubMedGoogle Scholar
  105. Ryan MG, Hubbard RM, Pongracic S, Raison RJ, McMurtrie RE (1996) Foliage, fine-root, woody-tissue and stand respiration in Pinus radiata in relation to nitrogen status. Tree Physiol 16:333–343.PubMedGoogle Scholar
  106. Safavi A, Rostamzadeh A, Maesum S (2006) Wide range pH measurements using a single H+-selective chromoionophore and a time-based flow method. Talanta 68:1469–1473.PubMedGoogle Scholar
  107. Sartoris FJ, Bock C, Serendero I, Lannig G, Portner HO (2003) Temperature-dependent changes in energy metabolism, intracellular pH and blood oxygen tension in the Atlantic cod. J Fish Biol 62:1239–1253.Google Scholar
  108. Schlüter U, Crawford RMM (2001) Long-term anoxia tolerance in leaves of Acorus calamus L. and Iris pseudacorus L. J Exp Bot 52:2213–2225.PubMedGoogle Scholar
  109. Schmälzlin E, Dongen JT van, Klimant I, Marmodée B, Steup M, Fisahn J, Geigenberger P, Löhmannsröben H-G (2005) An optical multifrequency phase-modulation method using microbeads for measuring intracellular oxygen concentrations in plants. Biophys J 89:1339–1345.PubMedGoogle Scholar
  110. Schurr U (1998) Xylem sap sampling–new approaches to an old topic. Trends Plant Sci 3:293–298.Google Scholar
  111. Schurr U, Walter A, Rascher U (2006) Functional dynamics of plant growth and photosynthesis–from steady-state to dynamics–from homogeneity to heterogeneity. Plant Cell Environ 29:340–352.PubMedGoogle Scholar
  112. Shui YB, Fu JJ, Garcia C, Dattilo LK, Rajagopal R, McMilllan S, Mak G, Holekamp NM, Lewis A, Beebe DC (2006) Oxygen distribution in the rabbit eye and oxygen consumption by the lens. Invest Ophthalmol Vision Sci 47:1571–1580.Google Scholar
  113. Sorz J, Hietz P (2006) Gas diffusion through wood: implications for oxygen supply. Trees 20:34–41.Google Scholar
  114. Spicer R, Holbrook NM (2005) Within-stem oxygen concentration and sap flow in four temperate tree species: does long-lived xylem parenchyma experience hypoxia? Plant Cell Environ 28:192–201.Google Scholar
  115. Stehning C, Holst GA (2004) Addressing multiple indicators on a single optical fiber–digital signal processing approaches for temperature compensated oxygen sensing. IEEE Sens J 4:153–159.Google Scholar
  116. Stern O, Volmer M (1919) Über die Abklingzeit der Fluoreszenz. Phys Z 20:183–188.Google Scholar
  117. Stumm W, Morgan JJ (1996) Aquatic chemistry: chemical equilibria and rates in natural waters. Wiley, New York.Google Scholar
  118. Tengberg A, Hovdenes J, Barranger D, Brocandel O, Diaz R, Sarkkula J, Huber C, Stangelmayer A (2003) Optodes to measure oxygen in the aquatic environment. Sea Technol 44:10–15.Google Scholar
  119. Tengberg A, Hovdenes J, Andersson HJ, Brocandel O, Diaz R, Hebert D, Arnerich T, Huber C, Kortzinger A, Khripounoff A, Rey F, Ronning C, Schimanski J, Sommer S, Stangelmayer A (2006) Evaluation of a lifetime-based optode to measure oxygen in aquatic systems. Limnol Oceanogr Methods 4:7–17.Google Scholar
  120. Teskey RO, McGuire MA (2002) Carbon dioxide transport in xylem causes errors in estimation of rates of respiration in stems and branches of trees. Plant Cell Environ 25:1571–1577.Google Scholar
  121. Teskey RO, McGuire MA (2005) CO2 transported in xylem sap affects CO2 efflux from Liquidambar styraciflua and Platanus occidentalis stems, and contributes to observed wound respiration phenomena. Trees 19:357–362.Google Scholar
  122. Teskey RO, McGuire MA (2007) Measurement of stem respiration of sycamore (Platanus occidentalis L.) trees involves internal and external fluxes of CO2 and possible transport of CO2 from roots. Plant Cell Environ 30:529–669.Google Scholar
  123. Thuesen EV, Rutherford LD, Brommer PL, Garrison K, Gutowska MA, Towanda T (2005a) Intragel oxygen promotes hypoxia tolerance of scyphomedusae. J Exp Biol 208:2475–2482.PubMedGoogle Scholar
  124. Thuesen EV, Rutherford LD, Brornmer PL (2005b) The role of aerobic metabolism and intragel oxygen in hypoxia tolerance of three ctenophores: Pleurobrachia bachel, Bolinopsis infundibulum and Mnemiopsis leidyi. J Mar Biol Assoc UK 85:627–633.Google Scholar
  125. Vasylevska AS, Borisov SM, Krause Ch, Wolfbeis OS (2006) Indicator-loaded and permeation-selective microparticles for use in fiber optic simultaneous sensing of pH and dissolved Oxygen. Chem Mater 18:4609–4616.Google Scholar
  126. Viollier E, Rabouille C, Apitz SE, Breuer E, Chaillou G, Dedieu K, Furukawa Y, Grenz C, Hall P, Janssen F, Morford JL, Poggiale JC, Roberts S, Shimmield T, Taillefert M, Tengberg A, Wenzhofer F, Witte U (2003) Benthic biogeochemistry: state of the art technologies and guidelines for the future of in situ survey. J Exp Mar Biol Ecol 285:5–31.Google Scholar
  127. Visser EJW, Bögemann GM, Van de Steeg HM, Pierik R, Blom WPM (2000) Flooding tolerance of Carex species in relation to field distribution and aerenchyma formation. New Phytol 148:93–103.Google Scholar
  128. Von Willert DJ, Matyssek R, Herppich W (1995) Experimentelle Pflanzenökologie. Thieme, Stuttgart.Google Scholar
  129. Walter A, Scharr H, Gilmer F, Zierer R, Nagel KA, Ernst M, Wiese A, Virnich O, Christ MM, Uhlig B, Jünger S, Schurr U (2007) Dynamics of seedling growth acclimation towards altered light conditions can be quantified via GROWSCREEN: a setup and procedure designed for rapid optical phenotyping of different plant species. New Phytol. DOI 10.1111/j.1469–8137.2007.02002.x.Google Scholar
  130. Werner T, Huber Ch, Heinl S, Kollmannsberger M, Daub J, Wolfbeis OS (1997) Novel optical pH-sensor based on a boradiaza-indacene derivative. Fresen J Anal Chem 359:150–154.Google Scholar
  131. Wießner A, Kuschk P, Stottmeister U (2002) Oxygen release by roots of Typha latifolia and Juncus effusus in laboratory hydroponic systems. Acta Biotechnol 22:209–216.Google Scholar
  132. Wilkinson S, Corlett JE, Oger L, Davies WJ (1998) Effects of xylem pH on transpiration from wild-type and flacca tomato leaves. Plant Physiol 117:703–709.PubMedGoogle Scholar
  133. Wolfbeis OS (2005) Materials for fluorescence-based optical chemical sensors. J Mater Chem 15:2657–2669.Google Scholar
  134. Wolfbeis OS, Dürkop A, Wu M, Lin Z (2002) A europium-ion-based luminescent sensing probe for hydrogen peroxide. Angew Chem Int Ed 41:4495–4498.Google Scholar
  135. Xu C, Wygladacz A, Qin Y, Retter R, Bell M, Bakker E (2005) Microsphere optical ion sensors based on doped silica gel templates. Anal Chim Acta 537:135–143.Google Scholar
  136. Zhu QZ, Aller RC, Fan YZ (2005) High-performance planar pH fluorosensor for two-dimensional pH measurements in marine sediment and water. Environ Sci Technol 39:8906–8911.PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2008

Authors and Affiliations

  • Dirk Gansert
    • 1
  • Stephan Blossfeld
    • 2
  1. 1.Department of Ecology & Ecosystem ResearchUniversity of GÖttingenGÖttingenGermany
  2. 2.Department of Ecological Plant Physiology & GeobotanyUniversity of DüsseldorfDüsseldorfGermany

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