Abstract
Taxus species in plant cell cultures are capable of producing the important anticancer agent taxol (paclitaxel) and related taxanes. Here we show that the combined effect of various enhancement strategies can stimulate the production of taxol and its semisynthetic progenitor baccatin III much more than any individual approach and that this can be particularly valuable in large-scale systems. Moreover, to achieve a better understanding of the taxol metabolism in order to develop rational strategies for the bioengineering of this taxane, mevinolin and fosmidomycin have been used in Taxus cell lines as tools to block HMG-CoA reductase and DOXP reductoisomerase in the mevalonate and non-mevalonate branch of the terpenoid biosynthetic pathway, respectively. Our results show that, in the synthesis of taxol, the non-mevalonate pathway is the main source of the universal terpenoid precursor isopentenyl diphosphate.
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(2007). Taxus. In: Pua, EC., Davey, M. (eds) Transgenic Crops VI. Biotechnology in Agriculture and Forestry, vol 61. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-71711-9_11
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DOI: https://doi.org/10.1007/978-3-540-71711-9_11
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