Abstract
This chapter describes the history of the development of soybean regeneration and transformation techniques and protocols, beginning from the 1970s. Techniques are described for the regeneration of soybean plants from cell cultures through somatic embryogenesis or shoot organogenesis from apical and axillary meristematic cells. Also presented are transformation methods and improvements that combine various regeneration techniques with DNA delivery methods, including Agrobacterium tumefaciens, particle bombardment, and electroporation-mediated gene transfer. Key publications in each area of research are given to exemplify the critical factors and advances in the technologies and the progression of ideas that have created current protocols used across laboratories today.
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References
Aragao FJL, Sarokin L, Vianna GR, Rech EL (2000) Selection of transgenic meristematic cells utilizing a herbicidal molecule results in the recovery of fertile transgenic soybean [Glycine max (L.) Merrill] plants at a high frequency. Theor Appl Genet 101:1–6
Bailey MA, Boerma HR, Parrott WA (1993) Genotype effects on proliferative embryogenesis and plant regeneration of soybean. In Vitro Cell Dev Biol 29P:102–108
Barwale UB, Kerns HR, Widholm JM (1986) Plant regeneration from callus cultures of several soybean genotypes via embryogenesis and organogenesis. Planta 167:473–481
Beversdorf WD, Bingham ET (1977) Degrees of differentiation obtained in tissue cultures of Glycine species. Crop Sci 17:307–311
Chee PP, Fober KA, Slightom JL (1989) Transformation of soybean (Glycine max) by infecting germinating seeds with Agrobacterium tumefaciens. Plant Physiol 91:1212–1218
Cheng TY, Saka T, Voqui-Dinh TH (1980) Plant regeneration from soybean cotyledonary node segments in culture. Plant Sci Lett 19:91–99
Chowrira GM, Akella V, Fuerst PE, Lurquin PF (1996) Transgenic grain legumes obtained by in planta electroporation-mediated gene transfer. Mol Biotechnol 5:85–96
Christianson ML, Warnick DA, Carlson PS (1983) A morphogenetically competent soybean suspension culture. Science 222:632–634
Christou P (1990) Morphological description of transgenic soybean chimeras created by the delivery, integration and expression of foreign DNA using electric discharge particle acceleration. Ann Bot 66:379–386
Clemente TE, LaVallee BJ, Howe AR, Conner-Ward D, Rozman RJ, Hunter PE, Broyles DL, Kasten DS, Hinchee MA (2000) Progeny analysis of glyphosate selected transgenic soybeans derived from Agrobacterium-mediated transformation. Crop Sci 40:797–803
Dan Y, Reichert NA (1998) Organogenic regeneration of soybean from hypocotyl explants. In Vitro Cell Dev Biol 34P:14–21
Delzer BW, Somers DA, Orf JH (1990) Agrobacterium tumefaciens susceptibility and plant regeneration of 10 soybean genotypes in maturity groups 00 to II. Crop Sci 30:320–322
Di R, Purcell V, Collins GB, Ghabrial SA (1996) Production of transgenic soybean lines expressing the bean pod mottle virus coat protein precursor gene. Plant Cell Rep 15:746–750
Donaldson PA, Simmonds DH (2000) Susceptibility to Agrobacterium tumefaciens and cotyledonary node transformation in short-season soybean. Plant Cell Rep 19:478–484
Droste A, Pasquali G, Bodanese-Zanettini MH (2000) Integrated bombardment and Agrobacterium transformation system: an alternative method for soybean transformation. Plant Mol Biol Rep 18:51–59
Dufourmantel N, Pelissier B, Garcon F, Peltier G, Ferullo J-M, Tissot G (2004) Generation of fertile transplastomic soybean. Plant Mol Biol 55:479–489
Ebert A, Taylor HF (1990) Assessment of the changes of 2,4-dichlorophenoxyacetic acid concentrations in plant tissue culture media in the presence of activated charcoal. Plant Cell Tissue Organ Cult 20:165–172
Finer JJ (1988) Apical proliferation of embryogenic tissue of soybean [Glycine max (L.) Merrill]. Plant Cell Rep 7:236–241
Finer JJ, McMullen MD (1991) Transformation of soybean via particle bombardment of embryogenic suspension culture tissue. In Vitro Cell Dev Biol 27P:175–182
Finer JJ, Nagasawa A (1988) Development of an embryogenic suspension culture of soybean (Glycine max Merrill.). Plant Cell Tissue Organ Cult 15:125–136
Franklin G, Carpenter L, Davis E, Reddy CS, Al-Abed D, Abou Alaiwi W, Parani M, Smith B, Goldman SL, Sairam RV (2004) Factors influencing regeneration of soybean from mature and immature cotyledons. Plant Growth Regul 43:73–79
Gamborg OL, Miller RA, Ojima K (1968) Plant cell cultures. I. Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50:151–158
Ghazi TD, Cheema HV, Nabors MW (1986) Somatic embryogenesis and plant regeneration from embryogenic callus of soybean, Glycine max L. Plant Cell Rep 5:452–456
Hammatt N, Davey MR (1987) Somatic embryogenesis and plant regeneration from cultured zygotic embryos of soybean (Glycine max L. Merr.). J Plant Physiol 128:219–226
Hansen G, Wright MS (1999) Recent advances in the transformation of plants. Trends Plant Sci 4:226–231
Hartweck LM, Lazzeri PA, Cui D, Collins GB, Williams EG (1988) Auxin-orientation effects on somatic embryogenesis from immature soybean cotyledons. In Vitro Cell Dev Biol 24:821–828
Hazel CB, Klein TM, Anis M, Wilde HD, Parrott WA (1998) Growth characteristics and transformability of soybean embryogenic cultures. Plant Cell Rep 17:765–772
Hinchee MAW, Connor-Ward DV, Newell CA, McDonnell RE, Sato SJ, Gasser CS, Fischhoff DA, Re DB, Fraley RT, Horsch RB (1988) Production of transgenic soybean plants using Agrobacterium-mediated DNA transfer. Bio/Technology 6:915–922
Hofmann N, Nelson RL, Korban SS (2004) Influence of media components and pH on somatic embryo induction in three genotypes of soybean. Plant Cell Tissue Organ Cult 77:157–163
Hu C-H, Wang L (1999) In planta soybean transformation technologies developed in China: procedure, confirmation and field performance. In Vitro Cell Dev Biol Plant 35:417–420
Jackson SA, Rokhsar D, Stacey G, Shoemaker RC, Schmutz J, Grimwood F (2006) Toward a reference sequence of the soybean genome: a multiagency effort. Crop Sci 46:55–61
Kaneda Y, Tabei Y, Nishimura S, Harada K, Akihama T, Kitamura K (1997) Combination of thidiazuron and basal media with low salt concentrations increases the frequency of shoot organogenesis in soybeans [Glycine max (L.) Merr.]. Plant Cell Rep 17:8–12
Kim J, LaMotte CE, Hack E (1990) Plant regeneration in vitro from primary leaf nodes of soybean (Glycine max) seedlings. J Plant Physiol 136:664–669
Kim J, Hack E, LaMotte CE (1994) Synergistic effects of praline and inorganic micronutrients and effects of individual micronutrients on soybean (Glycine max) shoot regeneration in vitro. J Plant Physiol 144:726–734
Kimball SL, Bingham ET (1973) Adventitious bud development of soybean hypocotyl segments in culture. Crop Sci 13:758–760
Ko T-S, Korban SS (2004) Enhancing the frequency of somatic embryogenesis following Agrobacterium-mediated transformation of immature cotyledons of soybean [Glycine max (L.) Merrill.]. In Vitro Cell Dev Biol Plant 40:552–558
Ko T-S, Lee S, Krasnyanski S, Korban SS (2003) Two critical factors are required for efficient transformation of multiple soybean cultivars: Agrobacterium strain and orientation of immature cotyledonary explant. Theor Appl Genet 107:439–447
Ko T-S, Lee S, Farrand SK, Korban SS (2004) A partially disarmed vir helper plasmid, pKYRT1, in conjunction with 2,4-dichlorophenoxyacetic acid promotes emergence of regenerable transgenic somatic embryos from immature cotyledons of soybean. Planta 218:536–541
Komatsuda T, Kaneko K, Oka S (1991) Genotype x sucrose interactions for somatic embryogenesis in soybean. Crop Sci 31:333–337
Lazzeri PA, Hildebrand DF, Collins GBA (1985) A procedure for plant regeneration from immature cotyledon tissue of soybean. Plant Mol Biol Rep 3:160–167
Lazzeri PA, Hildebrand DF, Collins GB (1987) Soybean somatic embryogenesis: effects of hormones and culture manipulations. Plant Cell Tissue Organ Cult 10:197–208
Lee A, Gatterdam PE, Chiu TY, Mallipudi M, Fiala RR (1991) Plant metabolism. In: Shaner DL, O’Connor SL (eds) The imidazolinone herbicides. CRC, Boca Raton, pp 151–165
Li Z, Nelson RL, Widholm JM, Bent A (2002) Soybean transformation via the pollen tube pathway. Soybean Genet Newsl 29:1–11
Lippmann B, Lippmann G (1984) Induction of somatic embryos in cotyledonary tissue of soybean, Glycine max L. Merr. Plant Cell Rep 3:215–218
Liu H-K, Yang C, Wei Z-M (2004) Efficient Agrobacterium tumefaciens-mediated transformation of soybeans using an embryonic tip regeneration system. Planta 219:1042–1049
Liu W, Moore PJ, Collins GB (1992) Somatic embryogenesis in soybean via somatic embryo cycling. In Vitro Cell Dev Biol 28P:153–160
Liu W, Torisky RS, McAllister KP, Avdiushko S, Hildebrand D, Collins GB (1996) Somatic embryo cycling: evaluation of a novel transformation and assay system for seed-specific gene expression in soybean. Plant Cell Tissue Organ Cult 47:33–42
Maliga P (2004) Plastid transformation in higher plants. Annu Rev Plant Biol 55:289–313
McCabe DE, Swain WF, Martinell BJ, Christou P (1988) Stable transformation of soybean (Glycine max) by particle acceleration. Bio/Technology 6:923–926
Meurer CA, Dinkins RD, Redmond CT, McAllister KP, Tucker DT, Walker DR, Parrott WA, Trick HN, Essig JS, Frantz HM, Finer JJ, Collins GB (2001) Embryogenic response of multiple soybean [Glycine max (L.) Merr.] cultivars across three locations. In Vitro Cell Dev Biol Plant 37:62–67
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Olhoft PM, Somers DA (2001) L-Cysteine increases Agrobacterium-mediated T-DNA delivery into soybean cotyledonary-node cells. Plant Cell Rep 20:706–711
Olhoft PM, Lin K, Galbraith J, Nielsen NC, Somers DA (2001) The role of thiol compounds in increasing Agrobacterium-mediated transformation of cotyledonary-node cells. Plant Cell Rep 20:731–737
Olhoft PM, Flagel LE, Donovan CM, Somers DA (2003) Efficient soybean transformation using hygromycin B selection in the cotyledonary-node method. Planta 216:723–735
Olhoft PM, Flagel LE, Somers DA (2004) T-DNA locus structure in a large population of soybean plants transformed using the Agrobacterium-mediated cotyledonary-node method. Plant Biotechnol J 2:289–300
Padgette SR, Kolacz KH, Delannay X, Re DB, LaVallee BJ, Tinius CN, Rhodes WK, Otero YI, Barry GF, Eichholtz DA, Peschke VM, Nida DL, Taylor NB, Kishore GM (1995) Development, identification, and characterization of a glyphosate-tolerant soybean line. Crop Sci 35:1451–1461
Palanichelvam K, Oger P, Clough SJ, Cha C, Bent AF, Farrand SK (2000) A second T-region of the soybean-supervirulent chrysopine-type Ti plasmid pTiChry5, and construction of a fully disarmed vir helper plasmid. Mol Plant Microbe Interact 13:1081–1091
Parrott WA, Clemente TE (2004) Transgenic soybean. In: Boerma HR, Specht JE (eds) Soybeans: improvement, production, and uses, 3rd edn. (Agronomy Monograph 16) American Society of Agronomy/Crop Science Society of America/Soil Science Society of America, Madison, pp 265–302
Parrott WA, Dryden G, Vogt S, Hildebrand DF, Collins GB, Williams EG (1988) Optimization of somatic embryogenesis and embryo germination in soybean. In Vitro Cell Dev Biol 24:817–820
Parrott WA, Hoffman LM, Hildebrand DF, Williams EG, Collins GB (1989) Recovery of primary transformants of soybean. Plant Cell Rep 7:615–617
Parrott WA, Durham RE, Bailey MA (1995) Somatic embryogenesis in legumes. In: Bajaj YPS (ed) Somatic embryogenesis and synthetic seed II. (Biotechnology in agriculture and forestry, vol 31). Springer, Berlin Heidelberg New York, pp 199–227
Paz MM, Shou H, Guo Z, Zhang Z, Banerjee AK, Wang K (2004) Assessment of conditions affecting Agrobacterium-mediated soybean transformation using the cotyledonary node explant. Euphytica 136:167–179
Ranch JP, Oglesby L, Zielinski AC (1985) Plant regeneration from embryo-derived tissue cultures of soybeans. In Vitro Cell Dev Biol 21:653–657
Reichert NA, Young MM, Woods AL (2003) Adventitious organogenic regeneration from soybean genotypes representing nine maturity groups. Plant Cell Tissue Organ Cult 75:273–277
Sairam RV, Franklin G, Hassel R, Smith B, Meeker K, Kashikar N, Parani M, Abed Al, Ismail S, Berry K, Goldman SL (2003) A study on the effect of genotypes, plant growth regulators and sugars in promoting plant regeneration via organogenesis from soybean cotyledonary nodal callus. Plant Cell Tissue Organ Cult 75:79–85
Saka H, Voqui-Dinh TH, Cheng T-Y (1980) Stimulation of multiple shoot formation on soybean stem nodes in culture. Plant Sci Lett 19:193–201
Samoylov VM, Tucker DM, Parrott WA (1998) Soybean [Glycine max (L.) Merrill] embryogenic cultures: the role of sucrose and total nitrogen content on proliferation. In Vitro Cell Dev Biol Plant 34:8–13
Sato S, Newell C, Kolacz K, Tredo L, Finer J, Hinchee M (1993) Stable transformation via particle bombardment in two different soybean regeneration systems. Plant Cell Rep 12:408–413
Schenk RU, Hildebrandt AC (1972) Medium and techniques for induction of growth of monocotyledonous and dicotyledonous plant cell cultures. Can J Bot 50:166–204
Shetty K, Asano Y, Oosawa K (1992) Stimulation of in vitro shoot organogenesis in Glycine max (Merrill.) by allantoin and amides. Plant Sci 81:245–251
Simmonds DH, Donaldson PA (2000) Genotype screening for proliferative embryogenesis and biolistic transformation of short-season soybean genotypes. Plant Cell Rep 19:485–490
Sleper DA, Shannon JG (2003) Role of public and private soybean breeding programs in the development of soybean varieties using biotechnology. AgBio Forum 6:27–32
Stacey G, Vodkin L, Parrott WA, Shoemaker RC (2004) National science foundation-sponsored workshop report. Draft plan for soybean genomics. Plant Physiol 135:59–70
Tomlin ES, Branch SR, Chamberlain D, Gabe H, Wright MS, Stewart CN Jr (2002) Screening of soybean, Glycine max (L.) Merrill, lines for somatic embryo induction and maturation capability from immature cotyledons. In Vitro Cell Dev Biol Plant 38:543–548
Townsend JA, Thomas LA (1994) An improved method of Agrobacterium-mediated transformation of cultured soybean cells. Patent WO9402620
Trick HN, Finer JJ (1997) SAAT: sonication-assisted Agrobacterium-mediated transformation. Transgenic Res 6:329–336
Trick HN, Finer JJ (1998) Sonication-assisted Agrobacterium-mediated transformation of soybean [Glycine max (L.) Merrill] embryogenic suspension culture tissue. Plant Cell Rep 17:482–488
Trick HN, Dinkins RD, Santarem ER, Di R, Samoylov V, Meurer C, Walker D, Parrott WA, Finer JJ, Collins GB (1997) Recent advances in soybean transformation. Plant Tissue Cult Biotechnol 3:9–26
Wilcox JR (2004) World distribution and trade of soybean. In: Boerma HR, Specht JE (eds) Soybeans: improvement, production and uses, 3rd edn. (Agronomy Monograph 16) American Society of Agronomy/Crop Science Society of America/Soil Science Society of America, Madison, pp 1–13
Wright MS, Koehler SM, Hinchee MA, Carnes MG (1986) Plant regeneration by organogenesis in Glycine max. Plant Cell Rep 5:150–154
Wright MS, Ward DV, Hinchee MA, Carnes MG, Kaufman RJ (1987a) Regeneration of soybean (Glycine max L. Merr.) from cultured primary leaf tissue. Plant Cell Rep 6:83–89
Wright MS, Williams MH, Pierson PE, Carnes MG (1987b) Initiation and propagation of Glycine max L. Merr.: Plants from tissue-cultured epicotyls. Plant Cell Tissue Organ Cult 8:83–90
Wright MS, Launis KL, Novitzdy R, Duesiing JH, Harms CT (1991) A simple method for the recovery of multiple fertile plants from individual somatic embryos of soybean [Glycine max (L.) Merrill]. In Vitro Cell Dev Biol 27P:153–157
Yan B, Srinivasa Reddy MS, Collins GB, Dinkins RD (2000) Agrobacterium tumefaciens-mediated transformation of soybean [Glycine max (L.) Merrill] using immature zygotic cotyledon explants. Plant Cell Rep 19:1090–1097
Yoshida T (2002) Adventitious shoot formation from hypocotyl sections of mature soybean seeds. Breed Sci 52:1–8
Zeng P, Vadnais DA, Zhang Z, Polacco JC (2004) Refined glufosinate selection in Agrobacterium-mediated transformation of soybean [Glycine max (L.) Merrill]. Plant Cell Rep 22:478–482
Zhang Z, Xing A, Staswick P, Clemente T (1999) The use of glufosinate as a selective agent in Agrobacterium-mediated transformation of soybean. Plant Cell Tissue Organ Cult 56:37–46
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(2007). Soybean. In: Pua, EC., Davey, M. (eds) Transgenic Crops VI. Biotechnology in Agriculture and Forestry, vol 61. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-71711-9_1
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DOI: https://doi.org/10.1007/978-3-540-71711-9_1
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