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DNA helicases in recombination

  • Chapter
Molecular Genetics of Recombination

Part of the book series: Topics in Current Genetics ((TCG,volume 17))

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Abstract

The different pathways of homologous recombination involve the recognition and pairing of homologous DNA sequences promoted by proteins that catalyze strand exchange. Other steps in recombination involve double stranded DNA unwinding for branch migration activities, and in some cases the reannealing of single DNA strands. These processes do not occur spontaneously and so require DNA helicase enzymes that unwind the DNA helices. DNA helicases also have additional and sometimes unexpected functions in homologous recombination. These include anti-recombination activities that either reverse strand pairings or destabilize the primary homologous recombination intermediate, a single stranded DNA molecule coated with Rad51 protein that is primed to promote strand exchange. This latter novel anti-recombinase activity is especially important during the repair of stalled replication forks. This review examines the roles of eukaryotic DNA helicases in promoting and antagonizing homologous recombination.

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  1. Department of Biochemistry, New York University School of Medicine, 550 First Avenue, New York, NY, 10016, USA

    Hannah L. Klein

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  1. Centro Andaluz de Biologia Molecular y Medicina Regenerativa CABIMER, CSIC-Universidad de Sevilla, Av. Américo Vespucio s/n, 41092, Sevilla, Spain

    Andrés Aguilera

  2. Department of Genetics & Development, Columbia University Medical Center, 701 West 168th Street, HHSC 1608, New York, NY, 10032-2704, USA

    Rodney Rothstein

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Klein, H.L. (2007). DNA helicases in recombination. In: Aguilera, A., Rothstein, R. (eds) Molecular Genetics of Recombination. Topics in Current Genetics, vol 17. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-71021-9_5

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