Skip to main content

Laboratory Practice for the Production of Polyclonal and Monoclonal Antibodies

  • Chapter
Advanced Techniques in Soil Microbiology

Part of the book series: Soil Biology ((SOILBIOL,volume 11))

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • BioChain Institute (2006) rProtein A-Sepharose product literature. BioChain Institute, Hayward

    Google Scholar 

  • Johnstone A, Thorpe R (1996) Immunochemistry in practice, 3rd edn. Blackwell Science, Oxford

    Google Scholar 

  • Köhler G, Milstein C (1975) Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 256:495–497

    Article  PubMed  Google Scholar 

  • Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685

    Article  PubMed  CAS  Google Scholar 

  • Page M, Thorpe R (1996) Purification of IgG using DEAE-sepharose chromatography. In: Walker JM (ed) The protein protocols handbook. Humana, Totowa, pp 725–726

    Google Scholar 

  • Roitt I, Brostoff J, Male D (1996) Immunology, 4th edn. Mosby, London

    Google Scholar 

  • Rosenberg IM (1996) Protein analysis and purification — benchtop techniques. Birkhauser, Boston

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2007 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Khurana, S., Bhaskar, S., Mukhopadhyay, A. (2007). Laboratory Practice for the Production of Polyclonal and Monoclonal Antibodies. In: Varma, A., Oelmüller, R. (eds) Advanced Techniques in Soil Microbiology. Soil Biology, vol 11. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-70865-0_5

Download citation

Publish with us

Policies and ethics