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mRNA Quantitation Using Real Time PCR

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Advanced Techniques in Soil Microbiology

Part of the book series: Soil Biology ((SOILBIOL,volume 11))

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References

  • Benoy IH, Elst H, Van der Auwera I, Van Laere S, Van Dam P, Van Marck E, Scharpe S, Vermeulen PB, Dirix LY (2004) Real-time RT-PCR correlates with immunocytochemistry for the detection of disseminated epithelial cells in bone marrow aspirates of patients with breast cancer. Br J Cancer 91:1813–1820

    Article  PubMed  CAS  Google Scholar 

  • Bustin SA (2000) Absolute quantitation of mRNA using real-time reverse transcription polymerase chain reaction assays. J Mol Endocrinol 25:169–193

    Article  PubMed  CAS  Google Scholar 

  • Bustin SA, Dorudi S (1998) Molecular assessment of tumour stage and disease recurrence using PCR-based assays. Mol Med Today 4:389–396

    Article  PubMed  CAS  Google Scholar 

  • Desjardin LE, Perkins MD, Wolski K, Horn S, Teixeira L, Chen Y, Johnson JL, Ellner JJ, Dietze R, Bates J, Cave MD, Eisenach KD (1999) Measurement of sputum mycobacterium tuberculosis messenger RNA as a surrogate for response to chemotherapy. Am J Resp Crit Care 160:203–210

    CAS  Google Scholar 

  • Gibson UE, Heid CA, Williams PM (1996) A novel method for real time quantitative RTPCR. Genome Res 6:995–1001

    Article  PubMed  CAS  Google Scholar 

  • Godfrey TE, Kelly LA (2005) Development of quantitative reverse transcriptase PCR assays for measuring gene expression. In: Keohavong P, Grant SG (eds) Molecular toxicology protocols. (Methods in molecular biology, vol 291) Humana, Totowa, pp 423–445

    Google Scholar 

  • Heid CA, Stevens J, Livak KJ, Williams PM (1996) Real time quantitative PCR. Genome Res 6:986–994

    Article  PubMed  CAS  Google Scholar 

  • Hill WE (1996) The polymerase reaction: applications for the detection of foodborne pathogens. Crit Rev Food Sci Nut 36:23–173

    Article  Google Scholar 

  • Hod Y (1992) A simplified ribonuclease protection assay. Biotechniques 13:852–854

    PubMed  CAS  Google Scholar 

  • Holodniy M (1994) Clinical application of reverse transcription-polymerase chain reaction for HIV infection. Clin Lab Methods 40:335–349

    Google Scholar 

  • Jothikumar N, Cromeans TL, Robertson BH, Meng XJ, Hill VR (2005) A broadly reactive one step real-time RT-PCR assay for rapid and sensitive detection of hepatitis E virus. J Virol Methods 131:65–71

    Article  PubMed  CAS  Google Scholar 

  • Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 25:402–408

    Article  PubMed  CAS  Google Scholar 

  • Lyamichev V, Brow MA, Dahlberg JE (1993) Structure specific endonucleolytic cleavage of nucleic acids by eubacterial DNA polymerases. Science 260:778–783

    Article  PubMed  CAS  Google Scholar 

  • Mansur NR, Meyer-Siegler K, Wurzer JC, Sirover MA (1993) Cell cycle regulation of the glyceraldehydes-3-phosphate dehydrogenase/uracil DNA glycosylase gene in normal human cells. Nucleic Acids Res 21:993–998

    Article  PubMed  CAS  Google Scholar 

  • Parker RM, Barnes NM (1999) mRNA detection by in situ and northern hybridization. Methods Mol Biol 106:247–283

    PubMed  CAS  Google Scholar 

  • PE Applied Biosystems (1997) Relative quantitation of gene expression. (User bulletin 2) Applied Biosystems, Foster City

    Google Scholar 

  • Raeymaekers L (1999) General principles of quantitative PCR. In: Kochanowski B, Reischl U (eds) Quantitative PCR protocols, Humana, Totowa, pp 31–41

    Chapter  Google Scholar 

  • Ramachandran C, Melnick SJ (1999) Multidrug resistance in human tumours — molecular diagnosis and clinical significance. Mol Diagn 4:81–94

    Article  PubMed  CAS  Google Scholar 

  • Rappolee DA, Mark D, Banda MJ, Werb Z (1988) Wound macrophage express TGF alpha and other growth factors in-vivo; analysis by mRNA phenotyping. Science 241:708–712

    Article  PubMed  CAS  Google Scholar 

  • Rychlik W, Spencer WJ, Rhoads RE (1990) Optimization of the annealing temperature for DNA amplification in vitro. Nucleic Acids Res 18:6409–6412

    Article  PubMed  CAS  Google Scholar 

  • Saccomanno CF, Bordonaro M, Chen JS, Nordstrom JL (1992) A faster ribonuclease protecrtion assay. Biotechniques 13:846–850

    PubMed  CAS  Google Scholar 

  • Schutz E, Ahsen von N (1999) Spreadsheet software for thermodynamic melting point prediction of oligonuleotide hybridization with and without mismatches. Biotechniques 27:1218–1224

    PubMed  CAS  Google Scholar 

  • Weis JH, Tan SS, Martin BK, Wittwer CT (1992) Detection of rare mRNAs via quantitative RT-PCR. Trends Genet 8:263–264

    Article  PubMed  CAS  Google Scholar 

  • Zamorano PL, Mahesh VB, Brann DW (1996) Quantitative RT-PCR for neuroendocrine studies. A minireview. Neuroendocrinology 63:397–407

    PubMed  CAS  Google Scholar 

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Gochhait, S., Bukhari, S.I., Bamezai, R.N.K. (2007). mRNA Quantitation Using Real Time PCR. In: Varma, A., Oelmüller, R. (eds) Advanced Techniques in Soil Microbiology. Soil Biology, vol 11. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-70865-0_4

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