Investigation of the Bone Cartilage Interface by CLSM

Abstract

A common problem for implants is the stress and the strain at the interface of implant and tissue. The reason for are different mechanical properties of biomaterial and tissue. This mechanical incompatibility might lead to the loosening of the implant. There are several strategies dealing with improving the interface of biomaterial and implants. Here we present data of the architecture of a natural interface that might serve to develop advanced surfaces for solid implants.

From partially decalcified human tali the cartilage could be peeled off. Both, the cartilage and the bone were investigated by Confocal Laser Scanning Microscopy (CLSM). The images give an impression of the roughness and structure of the interface. The resulting three dimensional pictures were quantified by the help of quantitative image analyse software. The quantitative analyse gives information about the real interface area, which is higher than the planar geometrical area, the ratio of bone and cartilage of the transition zone, and about the morphological structure of the interface.

Quantitative data on the amount and structure of the graded interface can be used to model the mechanical properties of the interface and finally might help to create a micro design of the implant surface ideal for osteointegration and optimal load transfer.