Abstract
In this study we have used a novel surface selective laser sintering (SSLS) technique to develop CAD/CAM designed scaffolds for bone tissue engineering. SSLS polylactic acid scaffolds were evaluated in vitro and in vivo as templates for human fetal femur-derived cell and adult human bone marrow stromal cell osteogenesis. Both cell types were cultured successfully on SSLS scaffolds with an increase in expression of alkaline phosphatase activity. Cell in-growth and Alcian blue/Sirius red positive staining of matrix deposition were observed on SSLS scaffolds in vitro in basal medium and osteogenic culture conditions. Similar results were observed in vivo with type I collagen expressed by cells on the scaffolds. In the critical sized femur segmental defect, SSLS scaffolds seeded with the cells enhanced significantly bone tissue regeneration.
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References
Hutmacher D.W., (2000) “Scaffolds in tissue engineering bone and cartilage”, Biomaterials, V. 21, pp.2529–2543.
Sachlos E. and Czernuszka J.T., (2003) “Making tissue engineering scaffolds work. Review on the application of solid freeform fabrication technology to the production of tissue engineering scaffolds”, European Cells and Materials, V. 5, pp.29–40.
Antonov E.N., Bagratashvili V.N., Howdle S.M., Konovalov A.N., Popov V.K., Shakesheff K.M. and Whitaker M.J., (2005) “Threedimensional bioactive and biodegradable scaffolds fabricated by surface-selective laser sintering”, Advanced Materials, V. 17, No. 3, 327–330.
Antonov E.N., Bagratashvili V.N., Howdle S.M., Konovalov A.N., Popov V.K. and Panchenko V.Ya., (2006) “Fabrication of polymer scaffolds for tissue engineering using surface selective laser sintering”, Laser Physics, V. 16, No. 5, pp.774–787.
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© 2008 Springer-Verlag Berlin Heidelberg
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Bagratashvili, V. et al. (2008). Osteogenesis on Surface Selective Laser Sintered Bioresorbable Scaffolds. In: Katashev, A., Dekhtyar, Y., Spigulis, J. (eds) 14th Nordic-Baltic Conference on Biomedical Engineering and Medical Physics. IFMBE Proceedings, vol 20. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-69367-3_4
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DOI: https://doi.org/10.1007/978-3-540-69367-3_4
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-69366-6
Online ISBN: 978-3-540-69367-3
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