Doubled Haploids

  • Ming-Tang ChangEmail author
  • Edward H. CoeJrEmail author
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 63)

Maize doubled haploid (DH) technology provides fixed, pure lines from a donor parent. Protocols for breeding of DH lines are available for over 250 crop species, and over 300 DH-derived cultivars have been developed in 12 species worldwide (Forster and Thomas, 2005). In maize, methods for inducing, selecting and doubling haploid plants are advanced and are in widespread use.

In a haploid plant, expression of positive or deleterious effects of genes for seed development, plant growth and function is unmasked, and plants that function effectively will have a better chance to grow to maturity and set seeds. Haploid plants that show good vigor in a natural environment will usually perform well as DH progenies under environmental stress. When doubled and brought to normal genetic balance, DH lines can be selected for agronomic traits, and testing can more accurately estimate yield potential and yield stability under different environments. The DH genome with its pure genetic makeup may still be challenged by environment in the absence of prior selective pressure. The characteristics of DH lines in theory are fixed and stable, and no further inbreeding depression should be observed from generation to generation, although spontaneous mutation or genomic changes caused by transposable elements cannot be avoided (Stadler 1951; Messing 2005).


Double Haploid Line Chromosome Doubling Haploid Plant Fertility Restoration Haploid Production 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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© Springer Science+Business Media, B.V 2009

Authors and Affiliations

  1. 1.BASF Plant Science LLC, ExSeed Genetics, 2901 South Loop Dr. Bldg 3 Suite 3800AmesUSA
  2. 2.University of Missouri, Curtis HallColumbiaUSA

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