Auszug
Die Kryokonservierung ist heute innerhalb der Reproduktionsmedizin ein integriertes Behandlungskonzept. Das Einfrieren so unterschiedlicher reproduktiver Zellen und Gewebe wie Spermatozoen, Eizellen, Embryonen, Hodengewebe und Eierstockgewebe ist aus kryobiologischer Sicht eine Herausforderung. Entsprechend sind für den jeweiligen Zell- oder Gewebetyp optimierte Strategien und Methoden gefordert. Das herkömmliche langsame Einfrierverfahren hat seit nahezu 20 Jahren einen festen Platz für Standardanwendungen, die trotz leichter Modifikationen nahezu unverändert sind. Neuere Verfahren, wie die Vitrifikation, rücken zusehends in das reproduktionsbiologische und -medizinische Bewusstsein. Im Folgenden sollen die verschiedenen kryobiologischen Techniken und ihre Relevanz für die reproduktionsmedizinische Praxis im Allgemeinen und für die verschiedenen reproduktionsbiologischen Objekte im Besonderen vorgestellt und diskutiert werden.
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Literatur
Bielanski A, Nadin-Davis S, Sapp T, Lutze-Wallace C (2000) Viral contamination of embryos cryopreserved in liquid nitrogen. Cryobiology 40:110–116
Bielanski A, Bergeron H, Lau PCK, Devenish J (2003) Microbial contamination of embryos and semen during long term banking in liquid nitrogen. Cryobiology 46:146–152
Cha KY, Yoon TK, Kim T, Chung HM (2004) Vitrification of human oocytes. In: Gardner DK, Weissmann A, Howles CM, Shoham Z (eds) Textbook of Assisted Reproductive Techniques, 2nd edn. Taylor & Francis, London New York, pp 257–266
Chung HM, Hong SW, Lim JM et al. (2000) In vitro blastocysts formation of human oocytes obtained from unstimulated and stimulated cycles after vitrification at various maturational stages. Fertil Steril 73: 545–551
Cremades N, Sousa M, Silva J et al. (2004) Experimental vitrification of human compacted morulae and early blastocysts using fine diameter plastic micropipettes. Hum Reprod 19:300–305
Duru NK, Öngürü Ö, Celasun B et al. (2001) Post-thaw texture analysis of slowly frozen and vitrified human ovarian cortex. Hum Reprod 16 (Abstract Book 1): 180
Fountain D, Ralston M, Higgins N et al. (1997) Liquid nitrogen freezers: a potential source of microbial contamination of haematopoietic stem cell components. Transfusion 37:585–591
Hawkins AE, Zuckerman MA, Briggs M et al. (1996) Hepatitis B nucleotide sequence analysis: linking an outbreak of acute hepatitis B to contamination of a cryopreservation tank. J Viral Methods 60:81–88
Isachenko V, Perez-Sanchez F, Isachenko V et al. (1998) Vitrification of GV-porcine oocytes with intact intracellular lipids: effect of the cryoprotectant saturation/dilution stepping, elevated temperature and cytoskeleton inhibitor. Cryobiology 36: 250–253
Isachenko E, Isachenko V, Rahimi G, Nawroth F (2003) Cryopreservation of human ovarian tissue by direct plunging into liquid nitrogen. Eur J Obstes Gynecol 108:186–193
Isachenko V, Isachenko E, Katkov II et al. (2004) Cryoprotectant-free cryopreservation of human spermatozoa by vitrification and freezing in vapor: effect on motility, DNA integrity, and fertilization ability. Biol Reprod 71:1167–1173
Isachenko V, Montag M, Isachenko E et al. (2005a) Aseptic technology of vitrification of human pronuclear oocytes using open-pulled straws. Hum Reprod 20:492–496
Isachenko V, Isachenko E, Montag M et al. (2005b) Clean technique for cryoprotectant-free vitrification of human spermatozoa. Reprod Biomedicine Online 10:350–354
Isachenko V, Montag M, Isachenko E et al. (2006) Aseptic vitrification of human germinal vesicle oocytes using dimethyl sulfoxide as a croprotectant. Fertil Steril, 85:741–747
Jelinkova L, Selman HA, Arav A et al. (2002) Twin pregnant alter vitrification of 2-pronuclei human Embryos. Fertil Steril 77:412–414
Katayama KP, Stele J, Kuwayama M et al. (2003) High survival rate of vitrified human oocytes results in clinical pregnancy. Fertil Steril 80: 223–224
Kuwayama M (2001) Vitrification of human oocytes and embryos. IVF update. Tokyo medical view Co: 230–234 (Japanese)
Lane M, Gardner DK (2001) Vitrification of mouse oocytes using a nylon loop. Mol Reprod Dev 58: 342–347
Martino A, Sponginess N, Leibo SP (1996) Development into blastocysts of bovine oocytes cryopreserved by ultrarapid cooling. Biol Reprod 54: 1059–1069
Montag M, van der Ven K, Dorn C et al. (2005) Birth following double cryopreservation of human oocytes at metaphase II and pronuclear stages. Fertil Steril in press
Mukaida T, Nakamura S, Tomiyama T et al. (2003) Vitrification of human blastocysts using cry loops: clinical outcome of 223 cycles. Hum Reprod 18: 384–391
Orief Y, Schultze-Mosgau A, Dafopoulos K, Al-Hasani S (2005) Vitrification: will it replace the conventional gamete cryopreservation techniques? Middle East Fertility Society Journal 10:171–184
Porcu E, Fabbri R, Damiano G et al. (2000) Cclinical experience and application of oocyte cryopreservation. Mol Cell Endocrinol 169:33–37
Rall WF, Fahy GM (1985) Ice-free cryopreservation of mouse embryos at −196°C by vitrification. Nature 313: 573–575
Rall WF (1992) Cryopreservation of oocytes and embryos: methods and applications. Anima Reprod Sic 28: 237–245
Schroeder AK, Banz C, Katalinic A, Al-Hasani S, Weiss JM, Diedrich K, Ludwig M (2002) Counselling on cryopreservation of pronucleated oocytes. Reprod Biomed Online 6: 69–74
Selman HA, El-Danasouri I (2002) Pregnancies derived from vitrified human zygotes. Fertil Steril 77: 422–423
Tender RS, Zuckerman MA, Goldtone AH et al. (1995) Hepatitis-B transmission from contaminated Cryopreservation tank. Lancet 346: 137–140
Trounson A, Mohr L (1983) Human pregnancy following cryopreservation, thawing and transfer of an eight-cell embryo. Nature 305:707–709
Vajta G, Kuwayama M, Holm P at al. (1998) Open pulled straw (OPS) vitrification: a new way to reduce cryoinjuries of bovine ova and embryos. Mol Reprod Dev 51:53–58
Vanderzwalmen P, Bertin G, Debauche C et al. (2003) Vitrification of human blastocyst with hemi-straw carrier: application of assisted hatching after thawing. Hum Reprod 18: 1504–1511
Wu J, Zhang L, Wang X (2001) In vitro maturation, fertilization and embryo development after ultrarapid freezing of immature human oocytes. Reproduction 121: 389–393
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Montag, M. et al. (2007). Methoden der Kryokonservierung in der Reproduktionsmedizin. In: Das Deutsche IVF-Register 1996–2006. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-49928-2_8
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DOI: https://doi.org/10.1007/978-3-540-49928-2_8
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