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The Use of Heat-Shock-Induced Ectopic Expression to Examine the Functions of Genes Regulating Development

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Heat Shock and Development

Part of the book series: Results and Problems in Cell Differentiation ((RESULTS,volume 17))

Abstract

The beauty of the fruitfly, Drosophila melanogaster, as a system for the study of development derives principally from its amenability to genetic analysis (Morgan 1926). Yet there remain situations where classical genetics fails to address important questions: mutagenesis is by nature random and constrained. Once a gene has been identified, it is usually desirable to alter the sequence of its gene product, or the timing and location of its expression. To do so in a directed manner requires new technology. One successful approach has been the adoption of heat shock promoters to induce ectopic and high-level expression of developmentally active genes in transgenic flies.

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Authors and Affiliations

  1. Department of Developmental Biology, Beckman Center, Standford University School of Medicine, Palo Alto, CA, 94305, USA

    Greg Gibson

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  1. Greg Gibson
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Editors and Affiliations

  1. Department of Molecular and Cell Biology, The University of Connecticut, 75 North Eagleville Road, 06269-3044, Storrs, CT, USA

    Lawrence Hightower

  2. Institut für Biochemie der Pflanzen, Weinberg 3, D-4050, Halle (O), Federal Republic of Germany

    Lutz Nover

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© 1991 Springer-Verlag Berlin Heidelberg

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Gibson, G. (1991). The Use of Heat-Shock-Induced Ectopic Expression to Examine the Functions of Genes Regulating Development. In: Hightower, L., Nover, L. (eds) Heat Shock and Development. Results and Problems in Cell Differentiation, vol 17. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-46712-0_4

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  • DOI: https://doi.org/10.1007/978-3-540-46712-0_4

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-662-21993-5

  • Online ISBN: 978-3-540-46712-0

  • eBook Packages: Springer Book Archive

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