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Actin Structure Function Relationships Revealed by Yeast Molecular Genetics

  • Chapter
Molecular Interactions of Actin

Part of the book series: Results and Problems in Cell Differentiation ((RESULTS,volume 32))

Abstract

The yeast Saccharomyces cerevisiae, has proven to be a powerful model organism in which to study actin function and structure-function relationships. This yeast has a single actin gene, a feature that greatly simplifies molecular-genetic and biochemical analysis of actin. Yeast actin is 88% identical to mammalian actin and its biochemical properties are similar to all other actins studied (Nefsky and Bretscher 1992). The ease of molecular genetics in yeast has allowed the generation of numerous site-specific actin mutants. Furthermore, the nonlethal actin mutants have been expressed in yeast as the sole source of actin (Shortle et al. 1984), allowing elucidation of biological importance via phenotypic analysis, and purification and biochemical characterization of mutant actins.

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Author information

Authors and Affiliations

  1. Department of Molecular and Cell Biology, University of California, 401 Barker Hall, Berkeley, California, 94720-3202, USA

    David G. Drubin

Authors
  1. Lisa D. Belmont
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  2. David G. Drubin
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Editor information

Editors and Affiliations

  1. Department of Anatomy and Histology, Institute for Biomedical Research, University of Sydney, 2006, Sydney, Australia

    Cristobal G. dos Remedios

  2. Department of Biochemistry, University of Minnesota, 55455, Minneapolis, MN, USA

    David D. Thomas

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© 2001 Springer-Verlag Berlin Heidelberg

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Belmont, L.D., Drubin, D.G. (2001). Actin Structure Function Relationships Revealed by Yeast Molecular Genetics. In: dos Remedios, C.G., Thomas, D.D. (eds) Molecular Interactions of Actin. Results and Problems in Cell Differentiation, vol 32. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-46560-7_8

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  • DOI: https://doi.org/10.1007/978-3-540-46560-7_8

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-53675-5

  • Online ISBN: 978-3-540-46560-7

  • eBook Packages: Springer Book Archive

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