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Assessment of Bone, Cartilage, Tendon and Bone Cells by Confocal Laser Scanning Microscopy

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Advanced Bioimaging Technologies in Assessment of the Quality of Bone and Scaffold Materials

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Abstract

Confocal laser scanning microscopy (CLSM) is a type of high-resolution and comparatively non-destructive fluorescence imaging technique that overcomes the limitations of conventional wide-field microscopy and facilitates the generation of high-resolution 3D images from relatively thick sections of tissue. In addition, CLSM enables the in situ characterization of tissue microstructure. Images generated by CLSM have been utilized for the study of articular cartilage, bone, muscle, tendon and ligament, and in the field of orthopaedics. More importantly, recent evolution in techniques and technologies have facilitated a relatively widespread adoption of this imaging modality, with increased “user friendliness” and flexibility; therefore, applications of CLSM exist in the rapidly advancing field of orthopaedic implants and the investigation of joint lubrication. Accordingly, this chapter focuses on the specific applications, as well as the recent and future direction of developments of CLSM in orthopaedic research in tissues of orthopaedic interest.

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Authors and Affiliations

  1. School of Mechanical Engineering, The University of Western Australia, Australia, Nedlands, 6009, WA, Australia

    Chris W. Jones

  2. Department of Orthopaedic Surgery, School of Surgery and Pathology, The University of Western Australia, Nedlands, 6009, WA, Australia

    Chris W. Jones, Kirk H. M. Yip, Jiake Xu & Ming-Hao Zheng

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  1. Chris W. Jones
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  2. Kirk H. M. Yip
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  3. Jiake Xu
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  4. Ming-Hao Zheng
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Correspondence to Ming-Hao Zheng .

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Editors and Affiliations

  1. Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong, Hong Kong SAR, China

    Ling Qin (Professor) & Kwok Sui Leung (Professor of Department of Orthopaedics & Traumatology) (Professor) &  (Professor of Department of Orthopaedics & Traumatology)

  2. University of California, San Francisco

    Harry K. Genant (Professor Emeritus, Chairman Emeritus and Board of Directors) (Professor Emeritus, Chairman Emeritus and Board of Directors)

  3. Synarc, Inc, USA

    Harry K. Genant (Professor Emeritus, Chairman Emeritus and Board of Directors) (Professor Emeritus, Chairman Emeritus and Board of Directors)

  4. Department of Radiology and Organ Imaging, The Chinese University of Hong Kong, Hong Kong SAR, China

    James F. Griffith

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© 2007 Springer-Verlag Berlin Heidelberg

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Jones, C.W., Yip, K.H.M., Xu, J., Zheng, MH. (2007). Assessment of Bone, Cartilage, Tendon and Bone Cells by Confocal Laser Scanning Microscopy. In: Qin, L., Genant, H.K., Griffith, J.F., Leung, K.S. (eds) Advanced Bioimaging Technologies in Assessment of the Quality of Bone and Scaffold Materials. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-45456-4_21

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  • DOI: https://doi.org/10.1007/978-3-540-45456-4_21

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  • Online ISBN: 978-3-540-45456-4

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