Abstract
Osteoporosis is a disorder of bone remodeling in adults, which results from decreased bone formation and/or increased bone resorption. Osteoblast and osteoclast are two major functional cells involved in bone remodeling. Based on the understanding of normal biological functions of bone cells, many traditional western anti-osteoporotic agents and some alternative Chinese herbs were tested with in vitro cultured osteoblasts and osteoclasts. The results showed that diphosphonate (Ibandronate), calcitonin (Elcatonin) and kidney-tonifying herb preparations could greatly inhibit the resorption activity of osteoclasts including decreased TRAP-positive multinucleated osteoclasts, inhibited resorption pits on dentine and osteoclast fusion index, and increased osteoclasts apoptosis, whereas rhPTH(1-34), calcitonin (Miacalcic) and Chinese herb extract Jinjier could significantly enhance bone-forming activity of osteoblasts including increased osteoblast proliferation, enhanced ALP activity and mineralization capability. This indicates that the established in vitro bone cellmodels, including osteoblasts and osteoclasts, provide a rapid and effective screening strategy for anti-osteoporotic agents.
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© 2007 Springer-Verlag Berlin Heidelberg
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Wang, HF., Jin, WA., Gao, JJ., Sheng, H. (2007). Changes of Biological Function of Bone Cells and Effect of Anti-osteoporosis Agents on Bone Cells. In: Qin, L., Genant, H.K., Griffith, J.F., Leung, K.S. (eds) Advanced Bioimaging Technologies in Assessment of the Quality of Bone and Scaffold Materials. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-45456-4_12
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DOI: https://doi.org/10.1007/978-3-540-45456-4_12
Publisher Name: Springer, Berlin, Heidelberg
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