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Molecular Biology of Plant Cell Transformation

  • Chapter
Differentiation of Protoplasts and of Transformed Plant Cells

Part of the book series: Results and Problems in Cell Differentiation ((RESULTS,volume 12))

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Abstract

Recent advances in plant tissue culture techniques, recombinant DNA technology, and bacterial genetics, have made it feasible to isolate specific genes, manipulate them in vitro, and introduce them into plant cells. This not only opens up the exciting possibility of genetically manipulating crop plants, but also provides a powerful tool for studying regulation of plant gene expression and, possibly, for molecular cloning of selectable plant genes from gene libraries. Transformation, in this review, refers to the stable introduction of foreign genetic material into cells. There are several potential plant transformation vectors, DNA vehicles required for the efficient introduction and replication of foreign genes in cells (reviewed by Howell 1982). However, only the Ti-plasmid, the casual agent of crown gall tumorigenesis and, to a much more limited extent, the cauliflower mosaic virus (CaMV) have been successfully used to propagate foreign sequences in plants.

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  1. Central Research and Development Department, E. I. du Pont de Nemours & Company, Wilmington, Delaware, 19898, USA

    N. S. Yadav

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  1. Institut für Pflanzenphysiologie, Königin-Luise-Straße 12-16, D-1000, Berlin 33, Germany

    J. Reinert

  2. Botanisches Institut der Universität, Olshausenstraße 40-60, D-2300, Kiel 1, Germany

    H. Binding

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Yadav, N.S. (1986). Molecular Biology of Plant Cell Transformation. In: Reinert, J., Binding, H. (eds) Differentiation of Protoplasts and of Transformed Plant Cells. Results and Problems in Cell Differentiation, vol 12. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-39836-3_5

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