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A Systematic Statistical Analysis of Ion Trap Tandem Mass Spectra in View of Peptide Scoring

  • Jacques Colinge
  • Alexandre Masselot
  • Jérôme Magnin
Part of the Lecture Notes in Computer Science book series (LNCS, volume 2812)

Abstract

Tandem mass spectrometry has become central in proteomics projects. In particular, it is of prime importance to design sensitive and selective score functions to reliably identify peptides in databases. By using a huge collection of 140 000+ peptide MS/MS spectra, we systematically study the importance of many characteristics of a match (peptide sequence/spectrum) to include in a score function. Besides classical match characteristics, we investigate the value of new characteristics such as amino acid dependence and consecutive fragment matches. We finally select a combination of promising characteristics and show that the corresponding score function achieves very low false positive rates while being very sensitive, thereby enabling highly automated peptide identification in large proteomics projects. We compare our results to widely used protein identification systems and show a significant reduction in false positives.

Keywords

Matrix Assisted Laser Desorption Ionization False Positive Rate Score Function True Positive Rate Systematic Statistical Analysis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2003

Authors and Affiliations

  • Jacques Colinge
    • 1
  • Alexandre Masselot
    • 1
  • Jérôme Magnin
    • 1
  1. 1.GeneProt Inc.MeyrinSwitzerland

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