Categorization of Microparticles and Biological Cells by Laser Diffractometry

  • P. H. Kaye
  • I. K. Ludlow
  • M. R. Milburn
Conference paper
Part of the Springer Series in Optical Sciences book series (SSOS, volume 31)


The potential of light-scatter measurements as a means of categorizing micro-particles or biological cells has been demonstrated [1–5]. In particular, light-scatter data recorded from suspensions have proved useful for size distribution and refractive index determination of narrow distribution suspensions [2,3]. However, where a broad or bimodal size distribution exists the value of light-scatter measurements from suspensions is limited because of the loss of interpretable scattered intensity information. Recording data from individual particles is therefore preferable, though in such an approach adequate data for categorization must be recorded from each particle in a sufficiently brief interval to allow the examination of a large and statistically valid number of particles. This report describes instrumentation which approaches this aim.


Penicillium Chrysogenum Bacterial Spore Scatter Angle Dielectric Structure Polystyrene Latex 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Wyatt, P. J., Applied Opt. 7, 10 (1968)CrossRefGoogle Scholar
  2. 2.
    Morris V. J., Coles H., and Jennings B., Nature(Lon) 249, 240 (1974)ADSCrossRefGoogle Scholar
  3. 3.
    Wyatt, P. J., Berkman, R., and Phillips D., J. Bacteriol. 110, 2 (1972)Google Scholar
  4. 4.
    Arndt-Jovin D. T., and Jovin, T. M., Febs. Lett. 44, 2, 247 (1974)CrossRefGoogle Scholar
  5. 5.
    Eisert, W. G., Ostertag, R., and Neimann, E., Rev. Sci. Instrum. 46, 1021 (1975)ADSCrossRefGoogle Scholar
  6. 6.
    Kerker, M., in “The Scattering of Light”, chaps.3–8, Academic Press, N.Y. 1969Google Scholar
  7. 7.
    Shimizu, K.,and Ishimaru A., 29th. ACEMB, Sheraton-Boston, USA. Nov. 1976Google Scholar
  8. 8.
    Ludlow, I. K.,and Kaye, P. H., J. Coll. Interface Sci., 69, 3 (1979)CrossRefGoogle Scholar
  9. 9.
    Phillips, D., Wyatt, P. J., and Berkman, R., J.Coll. Interface Sci. 34, 1 (1970)CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1982

Authors and Affiliations

  • P. H. Kaye
    • 1
  • I. K. Ludlow
    • 1
  • M. R. Milburn
    • 1
  1. 1.Department of PhysicsThe Hatfield PolytechnicHatfield, HertfordshireEngland

Personalised recommendations