Categorization of Microparticles and Biological Cells by Laser Diffractometry
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The potential of light-scatter measurements as a means of categorizing micro-particles or biological cells has been demonstrated [1–5]. In particular, light-scatter data recorded from suspensions have proved useful for size distribution and refractive index determination of narrow distribution suspensions [2,3]. However, where a broad or bimodal size distribution exists the value of light-scatter measurements from suspensions is limited because of the loss of interpretable scattered intensity information. Recording data from individual particles is therefore preferable, though in such an approach adequate data for categorization must be recorded from each particle in a sufficiently brief interval to allow the examination of a large and statistically valid number of particles. This report describes instrumentation which approaches this aim.
KeywordsPenicillium Chrysogenum Bacterial Spore Scatter Angle Dielectric Structure Polystyrene Latex
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