Abstract
This paper summarizes the work of my group over the past four years. Different resonance Raman techniques are described which are useful in studying intermediates of photolabile systems in the millisecond, microsecond, nanosecond, and picosecond time domains. These techniques are used to study two important photobiological systems: bacteriorhodopsin (bR) and carbonmonoxy-hemoglobin (HbCO). The summary of the results of applying these techniques to study the retinal system in bR is given and discussed in terms of what is known about its photochemical proton pump cycle. The main results are 1) the largest retinal configurational changes occur in the first step (the absorption step) and 2) the Schiff base proton in (math) ionizes in 40 μs (in the bL550 → bM412 step).
Modified versions of this paper are being submitted to three different invited publications: a) The Proceedings of the Sergio Porto Laser Memorial Meeting, Rio de Janeiro, Brazil, June 29-July 3, 1980; b) The Proceedings of the VIIIth International Congress on Photobiology, Strasbourg, France, July 21–25, 1980; c) a volume edited by Lester Packard on Visual Pigments and Purple Membranes. It is also being submitted as an abstract to the International Congress on Raman Spectroscopy, Ottawa, Canada, August 4–9, 1980.
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El-Sayed, M.A. (1981). Time-Resolved Resonance Raman Techniques for Intermediates of Photolabile Systems. In: Guimaraes, W.O.N., Lin, CT., Mooradian, A. (eds) Lasers and Applications. Springer Series in Optical Sciences, vol 26. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-38609-4_34
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DOI: https://doi.org/10.1007/978-3-540-38609-4_34
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