Abstract
In plant cell culture, the delivery of nutrition and gas (mainly oxygen) to the cells is the most important factor for viability. In this paper, we propose a poly(dimethylsiloxane) (PDMS)-based micro culture system that is designed to have good aeration. PDMS is known to have excellent air permeability, and through the experimental method, we investigated the relation between the degree of air delivery and the thickness of the PDMS sheet covering the culture chamber. We determined the proper thickness of the cover sheet, and cultured protoplasts of Nicotiana tabacum in a culture chamber covered with a PDMS sheet whose thickness 400 µm. The cells were successfully divided, and lived well inside the culture chamber for 10 days. In addition, protoplasts were cultured inside the culture chambers covered with the coverglass and the PDMS sheet respectively, and the microcolonies were formed well inside the PDMS covered chamber after 10 days.
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References
Kamholz, AE, Weigl BH, Finlayson BA & Yager P (1999) Quantitative analysis of molecular interaction in a microfluidic channel: the T-sensor. Anal. Chem. 71:5340–5347
Hong JW, Studer V, Hang G, Anderson WF & Quake SR (2004) A nanoliter-scale nucleic acid processor with parallel architecture. Nature Biotechnology 22:435–439
Leclerc E, Sakai Y & Fujii T (2003) Cell Culture in 3-Dimensional Microfluidic Structure of PDMS (polydimethylsiloxane) Biomedical Microdevices 5:109–114
Denoual MJ, Aoki K, M-Tixier A & Fujita H (2003) A Microfluidic device for long-term study of individual cells. Proc. of microTAS 531–534
Tai Hyun Park & Michael L. Shuler (2003) Intergration of Cell Culture and Microfabrication Technology. Biothchnol. Prog. 19: 243–253
D. R. Albrecht, V.L. Tsang, R.L. Saha, S.N. Bhatia (2005) Photo-and electropattering of hydrogel-encapsulated living cell arrays. Lab Chip 5:111–118
Leclerc E, Sakai Y & Fujii T (2003) Fetal Human Hepatocytes and Endotheial Cells co-culture in a microfluidic environment. Proc. of microTAS 271–274
Walker GM, Ozers MS & Beebe DJ (2002) Insect Cell Culture in Microfluidic Channels. Biomedical Microdevices 4:161–166
Paul Van der Meeren, Dries De Vleeschauwer & Pierre Debergh (2001) Determination of oxygen profiles in agar-based gelled in vitro plant tissue culture media. Plant Cell, Tissue and Organ Culture 65:239–245
Charati SG & Stern SA (1998) Macromolecules Diffusion of Gases in Silicone Polymers. Molecular Dynamics Simulations. 31:5529–5535
Kim DJ, Oh HJ, Park TH, Choo JB & Lee SH (2005) An easily integrative and efficient micromixer and its application to the spectroscopic detection of glucose-catalyst reactions. Analyst 130:293–298
Sun YH, Xue QZ, Ding CM, Zhang XY, Zhang LL, Wang WF & Ali S (2005) Somatic cybridization between Nicotiana tabacum and N. repanda based on a single inactivation procedure of nuclear donor parental protoplasts. Plant Science 168: 303-308IFMBE at http://www.ifmbe.org
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© 2007 International Federation for Medical and Biological Engineering
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Ju, J., Ko, JM., Cha, HC., Lee, S. (2007). Poly(dimethylsiloxane)-Based Micro Chamber with Air Permeable Cover Sheet for the Protoplast of Nicotiana tabacum Culture. In: Magjarevic, R., Nagel, J.H. (eds) World Congress on Medical Physics and Biomedical Engineering 2006. IFMBE Proceedings, vol 14. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-36841-0_83
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DOI: https://doi.org/10.1007/978-3-540-36841-0_83
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-36839-7
Online ISBN: 978-3-540-36841-0
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