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Hirschsprung's Disease and Allied Disorders pp 199–206Cite as

NADPH-Diaphorase Histochemistry

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Abstract

The introduction of rectal suction biopsy, while making the procedure less traumatic for the patient, has made the diagnosis of Hirschsprung’s disease (HD) more difficult for the pathologist. Many histopathologists are reluctant to make a positive diagnosis of HD on the basis of suction rectal biopsy results, using conventional H&E stains. This reluctance stems from doubt as to the amount of submucosa that must be scanned before the absence of ganglion cells can be confirmed, as well as the relative difficulty in accurately identifying smaller and sparse submucosal ganglion cells by comparison with the more compact and familiar ganglion cells of the intermuscular plexus.

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Author information

Authors and Affiliations

  1. Department of Paediatric Surgery, University of Leipzig, 04103, Leipzig, Germany

    U. Rolle

  2. Children’s Research Centre, Our Lady’s Children’s Hospital, University College of Dublin, Crumlin, Dublin 12, Ireland

    P. Puri

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  1. U. Rolle
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  2. P. Puri
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Editor information

Editors and Affiliations

  1. Immenzaun 6a, 51429, Bergisch Gladbach, Germany

    Alexander Matthias Holschneider

  2. The Children’s Hospital of Cologne, Amsterdamerstr. 59, 50735 , Cologne, Germany

    Alexander Matthias Holschneider

  3. Our Lady’s Children’s Hospital, Children’s Research Centre, University College of Dublin, Crumlin, Dublin 12, Ireland

    Prem Puri

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© 2008 Springer-Verlag Berlin Heidelberg

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Rolle, U., Puri, P. (2008). NADPH-Diaphorase Histochemistry. In: Holschneider, A., Puri, P. (eds) Hirschsprung's Disease and Allied Disorders. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-33935-9_14

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  • DOI: https://doi.org/10.1007/978-3-540-33935-9_14

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-33934-2

  • Online ISBN: 978-3-540-33935-9

  • eBook Packages: MedicineMedicine (R0)

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