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Multiple Locus VNTR (Variable Number of Tandem Repeat) Analysis

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Molecular Identification, Systematics, and Population Structure of Prokaryotes

Abstract

The present chapter will review the current state of the art in the field of bacterial strain typing through the use of tandem repeat polymorphism. We will first go through a brief overview of multiple locus VNTR analysis (MLVA) typing and then describe how to set-up or enrich a MLVA assay. We will also review representative examples of the currently proposed MLVA assays and discuss the methods used for MLVA data analysis. Finally, we will compare MLVA to other approaches, and discuss issues related to standardisation and possibilities offered by the internet in terms of shared databases for MLVA (MLVA web services).

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Author information

Authors and Affiliations

  1. Division of Analytical Microbiology, Centre d’Etudes du Bouchet, B.P. 3, 91710, Vert le Petit, France

    Gilles Vergnaud

  2. GPMS laboratory, Institute of Genetics and Microbiology, University Paris XI, 91405, Orsay cedex, France

    Christine Pourcel

Authors
  1. Gilles Vergnaud
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  2. Christine Pourcel
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Editors and Affiliations

  1. DSMZ GmbH, Macheroder Weg 1b, 38124, Braunschweig, Germany

    Erko Stackebrandt

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© 2006 Springer-Verlag Berlin Heidelberg

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Vergnaud, G., Pourcel, C. (2006). Multiple Locus VNTR (Variable Number of Tandem Repeat) Analysis. In: Stackebrandt, E. (eds) Molecular Identification, Systematics, and Population Structure of Prokaryotes. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-31292-5_4

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