Abstract
We designed a retroviral computer, of which hardware is composed of DNA/RNA dependent DNA polymerase, transcriptase, RNaseH, and DNA and RNA strands. Sequences of DNA strands define functions and RNA single strands work as arguments and return values for each function. In this paper, we show that computational jobs, such as encoding of input data and AND/OR operation, can work on this computer. By combining multiple functions, more complex molecular programs for gene analysis can be constructed. Experimental study showed that some functions were actually executed in vitro autonomously. Since this computer has originally derived from the retrovirus mechanism, we expect an in vivo computer will be realized from this technology, which detects the cell state through gene expression patterns, and controls the cell conditions with output RNA. It may provide a powerful tool for both research and clinical application.
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Nitta, N., Suyama, A. (2004). Autonomous Biomolecular Computer Modeled after Retroviral Replication. In: Chen, J., Reif, J. (eds) DNA Computing. DNA 2003. Lecture Notes in Computer Science, vol 2943. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-24628-2_20
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DOI: https://doi.org/10.1007/978-3-540-24628-2_20
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