Abstract
The inclusion of alfalfa meal (sun-cured alfalfa hay, Medicago sativa) in the diet of cholesterol-fed cynomolgus macaques lowers cholesterolemia, normalizes the relative concentrations of plasma lipoproteins, and induces the regression of aortic and coronary atherosclerosis (Malinow et al., 1978b). It seems likely that alfalfa saponins are responsible for these beneficial effects since saponins from other sources reduce cholesterolemia in chicks (Griminger and Fisher, 1958; Newman et al., 1958; Morgan et al., 1972), pigs (Topping et al., 1980a), and rats (Oakenfull et al., 1979; Topping et al., 1980b). Research in this direction, however, has been hampered by the difficulty of obtaining sufficient amounts of alfalfa saponins devoid of contaminating substances. We have prepared an extract from alfalfa hay that contains saponins identifiable by thin-layer chromatography (unpublished data) and we have enhanced its capacity to interfere with cholesterol absorption by partial acid hydrolysis (Malinow et al., 1977). Although the extract may also contain unspecified amounts of carbohydrates, amino acids, peptides, pigments, and free aglycones (unpublished data) removed from alfalfa hay by the water: alcohol solvent used during its preparation, we have named the crude extract alfalfa saponins as an operational definition. These alfalfa saponins reduce the intestinal absorption of cholesterol in rats (Malinow et al., 1977) and monkeys (Malinow et al., 1981; they also decrease cholesterolemia in monkeys and increase the fecal excretion of neutral steroids and bile acids (Malinow et al., 1981).
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References
Applebaum, S.W., Marco, S., and Birk, Y.: Saponins as possible factors of resistance of legume seeds to the attack of insects. J. Agric. Food Chem. 17: 618–622, 1969.
Armstrong, M.L., Warner, E.D., and Connor, W.E.: Regression of coronary atheromatosis in rhesus monkeys. Circ. Res. 27: 59–67, 1970.
Bardana, E.J., Bayrakci, C., Pirofsky, B., and Jenjyoji, H.: The use of heparin in autoimmune hemolytic diseases. Blood 35: 377–385, 1970.
Bennett, R.M., and Molina, E.: The measurement of DNA antibodies. Am. J. Clin. Path. 65: 364 – 267, 1976.
Berrang, B., Davis, K.H., Jr., Wall, M.E., Hanson, C.H., and Pedersen, M.E.: Saponins of two alfalfa cultivars. Phytochemistry 13: 2253–2260, 1974.
Birk, Y.: Saponins. In: Toxic Constituents of Plant Foodstuffs, I. E. Liener, ed., Academic Press, New York, 1969, pp. 169–210.
Bond, M.G., Adams, M.R., and Bullock, B.C.: Complicating factors in evaluating coronary artery atherosclerosis. Artery 9: 21–29, 1981.
Bondi, A., Birk, Y., and Gestetner, F.: Forage saponins. In: Chemistry and Biochemistry of Herbage, Vol. 1, Butler, G.W. and Bailey, R.W., eds., Academic Press, New York, 1973, pp. 511–528.
Bunting, C.H.: Experimental anemias in rabbits. J. Exp. Med. 8: 625–646, 1906.
Burstein, M., Scholnick, H.R., and Morfin, R.: Rapid method for the isolation of lipoprotein from human serum by precipitation with polyanions. J. Lipid Res. 11: 583–595, 1970.
Cheeke, P.R.: Nutritional and physiological properties of saponins. Nutr. Rept. Int. 13: 315–324, 1976.
Clarkson, T.B., Lehner, N.D.M., Wagner, W.D., StClair, R.W., Bond, M.C., and Bullock, B.C.: Study of atherosclerosis regression in Macaca mulatto. 1. Design of experiment and lesion induction. Exp. Mol. Pathol. 30: 360–385, 1979. eds., Springer-Verlag, New York, 1980, pp. 739–748.
Clarkson, T.B., Lehner, N.D.M., Wagner, W.D., St. Clair, R.W., Bond, M.C., and Bulloci, B.C.: Study of atherosclerosis regression in Macaca mulatto. 1. Design of experiment and lesion induction. Exp. Mol. Pathol. 39: 360–385, 1979.
Ewart, A.J.: The poisonous action of ingested saponins. Council Sci. Industr. Res. (Australia) Bull. #50, 1931.
Gestetner, B., Assa, Y., Henis, Y., Birk, Y., and Bondi, A.: Lucerne saponins. IV. Relationship between their chemical constitution, and haemolytic and antifungal activities. J. Sci. Food Agric. 22’: 168–172, 1971.
Gestetner, B., Assa, Y., Henis, Y., Tencer, Y., Rotman, M., Birk, Y., and Bondi, A.: Interactions of lucerne saponins with sterols. Biochim. Biophys. Acta 270: 181–187, 1972.
Griminger, P., and Fisher, H.: Dietary saponin and plasma cholesterol in the chicken. Proc. Soc. Exp. Biol. Med. 99: 424–426, 1958.
Keys, A., Grande, F., and Anderson, J.T.: Bias and misrepresentation revisited: Perspective on saturated fat. Am. J. Clin. Nutr. 27: 188–212, 1974.
Kim, D.N., Lee, K.T., Reiner, J.M., and Thomas, W.A.: Steroid excretion in swine fed highfat, high-cholesterol diet with soy protein. Exp. Molec. Pathol. 33: 25–35, 1980.
Lynch’s Medical Laboratory Technology, 3rd edition, Raphael, S.S., senior author, W.B. Saunders, Philadelphia, London, and Toronto, 1976.
Malinow, M.R.: Important considerations for nonhuman primate models of diet-induced atherosclerosis. In: Primates in Nutritional Research, Hayes, K.C., ed., Academic Press, New York and London, 1979, pp. 199–214.
Malinow, M.R.: Atherosclerosis: Regression in nonhuman primates. Circ. Res. 46: 311–320, 1980a.
Malinow, M.R.: Regression of atherosclerosis in nonhuman primates. An overview. In: Use of Nonhuman Primates in Cardiovascular Disease, Kalter, S.S., ed., University of Texas Press, Austin and London, 1980b, pp. 181–220.
Malinow, M.R.: Dietary and pharmacological control of atherosclerosis regression in cynomolgus macaques (Macaca fascicularis). Artery 9: 11–19, 1981.
Malinow, M.R., Connor, W.E., McLaughlin, P., Stafford, C., Lin, D.S., Livingston, A.L., Kohler, C.O., and McNulty, W.P.: Sterol balance in Macaca fascicularis: Effects of alfalfa saponins. J. Clin. Invest. 67: 156–162, 1981a.
Malinow, M.R., and Corcoran, A.: Growth of the lens in howler monkeys (Alouatta caraya). J. Mammal. 47: 58–63, 1966.
Malinow, M.R., McLaughlin, P., McNulty, W.P., Naito, H.K., and Lewis, L.A.: Treatment of established atherosclerosis during cholesterol feeding in monkeys. Atherosclerosis 31: 185–193, 1978a.
Malinow, M.R., McLaughlin, P., Naito, H.K., Lewis, A., and McNulty, W.P.: Effect of alfalfa meal on shrinkage (regression) of atherosclerotic plaques during cholesterol feeding in monkeys. Atherosclerosis 30: 27–43, 1978b.
Malinow, M.R., McLaughlin, P., Papworth, L., Naito, H.K., Lewis, L., and McNulty, W.P.: A model for therapeutic intervention on established coronary atherosclerosis in a nonhuman primate. Adv. Exp. Med. Biol. 67: 3–31, 1976.
Malinow, M.R., McLaughlin, P., Papworth, L., Stafford, C., Kohler, C.O., Livingston, A.L., and Cheeke, P.R.: Effect of alfalfa saponins on intestinal cholesterol absorption in rats. Am. J. Clin. Nutr. 30: 2061–2067, 1977.
Malinow, M.R., McLaughlin, P., and Perley, A.M.: The effect of pyridinol-carbamate on induced atherosclerosis in cynomolgus monkeys (Macaca ira). Atherosclerosis 15: 31–36, 1972.
Malinow, M.R., McNulty, W.P., McLaughlin, P., Stafford, C., Burns, A.K., Livingston, A.L., and Kohler, C.O.: The toxicity of alfalfa saponins in rats. Food Cosmet. Toxicol. 19: 443–445, 1981 b.
Morgan, B., Heald, M., Brooks, S.C., Tee, J.L., and Green, J.: The interations between dietary saponin, cholesterol, and related sterols in the chick. Poultry Sci. 51: 677–682, 1972.
Newman, H.A.I., Kummerow, F.A., and Scott, H.M.: Dietary saponins, a factor which may reduce liver and serum cholesterol levels. Poultry Sci. 37: 42–46, 1958.
Oakenfull, D.: Saponins in food. Food Chemistry 6: 19–40, 1981.
Oakenfull, D.C., Fenwick, D.E., Hood, R.L., Topping, D.L., Illman, R.L. and Storer, G.B.: Effect of saponins on bile-acids and plasma lipids in the rat. Br. J. Nutr. 41: 209–216, 1979.
Parkhurst, R.M., Thomas, D.W., and Skinner, W.A.: Molluscicidal saponins of Phytolacca dodecandra: Lemmatoxin. Can. J. Chem. 52: 702–705, 1974.
Pirofsky, B., Nelson, H., Imel, T., and Cordova, M.: The present status of the antiglobulin and bromelin tests in demonstrating erythrocyte antibodies. Am. J. Clin. Pathol. 36: 492–499, 1961.
Potter, J.D., Illman, R.J., Calvert, C.D., Oakenfull, D.C., and Topping, D.L.: Soya saponins, plasma lipids, lipoproteins and fecal bile acids. A double-blind crossover study. Nutr. Rept. Int. 22: 521–528, 1980.
Reshef, C., Gestetner, B., Birk, Y., and Bondi, A., Effect of alfalfa saponins on the growth and some aspects of lipid metabolism of mice and quails. J. Sci. Food Agric. 27: 63–72, 1976.
Rudel, L.L., and Morris, M.D.: Determination of cholesterol using o-phthalaldehyde. J. Lipid Res. 14: 364–366, 1973.
Shoppee, C.W.: Saponins and sapogenins. In: Chemistry of the Steroids, 2nd edition, Butterworths, London, 1964, pp. 398–432.
Sokal, R.R., and Rohlf, F.J.: Biometry. The Principles and Practice of Statistics in Biological Research. W.H. Freeman Company, San Francisco, 1969.
Stary, H.C., and Strong, J.P.: The fine structure of nonatherosclerotic intimai thickening, of developing, and of regressing atherosclerotic lesions at the bifurcation of the left coronary artery. Adv. Exp. Med. Biol. 67: 89–108, 1976.
Tan, E.M.: Relationship of nuclear staining patterns with precipitating antibodies in systemic lupus erythematosus. J. Lab. Clin. Med. 70: 800–812, 1967.
Tan, E.M., Rodaan, G.P., Garcia, I., Moroi, Y., Fritzler, M.J., and Peebles, C: Diversity of antinuclear antibodies in progressive systemic sclerosis. Arthritis Rheum. 23: 617–625, 1980.
Tetrasorb-125 Instruction Manual, Abbott Laboratories, Radio-Pharmaceutical Products Division, North Chicago, Illinois.
Thompson, C.R., van Atta, G.R., Bickhoff, E.M., Walter, E.D., Livingston, A.L., and Guggolz, J.: Preparation and chemistry of legume saponins. Tech. Bull. U.S. Agric. 1161: 63–81, 1957.
Topping, D.L., Storer, G.B., Calvert, G.D., Illman, R.J., Oakenfull, D.G., and Weller, R.A. Effects of dietary saponins on fecal bile acids and neutral sterols, plasma lipids, and lipoprotein turnover in the pig. Am. J. Clin. Nutr. 33: 783–786, 1980a.
Topping, D.L., Trimble, R.P., Illman, R.J., Potter, J.D., and Oakenfull, D.G.: Prevention of dietary hypercholesterolemia in the rat by soy flour high and low in saponins. Nutr. Rept. Int. 22: 513–519, 1980b.
van Atta, G.R., Guggolz, J., and Thompson, C.R.: Determination of saponins in alfalfa. J. Agric. Food Chem.9: 77–79, 1961.
Vesselinovitch, D., Wissler, R.W., Hughes, R., and Borensztajn, J.: Reversal of advanced atherosclerosis in rhesus monkeys. Atherosclerosis 23: 155–176, 1976.
Wissler, R.W., Vesselinovitch, D., Borensztajn, J., and Hughes, R.: Regression of severe atherosclerosis in cholestyramine-treated rhesus monkeys with or without a low-fat, low-cholesterol diet. Circulation 52 (Suppl. II): II-16, 1975 (abstract).
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Malinow, M.R., McLaughlin, P., Stafford, C., Livingston, A.L., Senner, J.W. (1983). Effects of Alfalfa Saponins on Regression of Atherosclerosis in Monkeys. In: Clinical Implications of Recent Research Results in Arteriosclerosis. Abhandlungen der Rheinisch-Westfälischen Akademie der Wissenschaften, vol 70. VS Verlag für Sozialwissenschaften, Wiesbaden. https://doi.org/10.1007/978-3-322-98699-3_20
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DOI: https://doi.org/10.1007/978-3-322-98699-3_20
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