Abstract
Three cases are presented where the rational design of transduction chemistries has led to improved catalytic and affinity electrochemical biosensors for environmental applications. Firstly, the improvement of the reductive recycling of tyrosinase-produced quinones by means of rational modification of electrode surfaces is demonstrated resulting in two orders of magnitude lowering of detection limits, and more than one order of magnitude improvement of the life time of phenolics sensors. Secondly, a phosphorylase A-phosphoglucomutase-glucose 6-phosphate dehydrogenase biosensor is demonstrated, that based on the use of this three-enzyme cascade and combined with new NADH oxidation mediators makes possible reagentless biosensors for phosphate detection. Thirdly, an immunosensor for atrazine is presented that based on electrochemically “wired” peroxidase-labelled atrazine and its competition for the binding sites of immobilised antibodies, reached µg 1−1 (ppb) detection limits and incubation times of minutes.
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© 1998 B. G. Teubner Verlagsgesellschaft Leipzig
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Narváez, A., López, M.A., González, E., Domínguez, E., Fernández, J.J., Katakis, I. (1998). Catalytic and Affinity Amperometric Biosensors for Phenols, Phosphates, and Atrazine: How Transduction Can Improve Performance. In: Hock, B., Barceló, D., Cammann, K., Hansen, PD., Turner, A.P.F. (eds) Biosensors for Environmental Diagnostics. Teubner-Reihe UMWELT. Vieweg+Teubner Verlag. https://doi.org/10.1007/978-3-322-93454-3_6
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DOI: https://doi.org/10.1007/978-3-322-93454-3_6
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