Abstract
A complication emerges when antigens and antibodies interact in continuous-use immunosensor systems. This complication comprises the regeneration of the biological sensing surface. In the present work we report the development and the study of two strategies designed to overcome this limitation.
The first strategy is based on the construction of amperometric immunosensors using rigid immunocomposites. These materials contain a conducting polymer composite that acts as a support for the bulk-immobilized immunological material. The surface of these immunosensors is renewable. A simple polishing procedure uncovers a fresh immunocomposite surface ready for a new immunoassay. This contrasts with conventional, single-use devices. Furthermore, immunosensors of different sizes and shapes can be produced using these immunocomposites. The closeness between the immunoconjugate enzyme-label and the conducting sites on the surface of the sensor yields a higher electron transfer efficiency. This is clearly convenient when building amperometric devices. The simplicity of this strategy makes it particularly convenient for manual immunoassay methodologies.
The second strategy is based on an immunochemical analysis system featuring flow injection techniques. This system uses potentiometric detection with immunochemical reagents immobilized on magnetic particles where the sensing surface can be renewed after each analysis. Measurements are reproducible since the magnetic particles can be fixed to the surface of the sensor at will. The regeneration of the sensing surface is achieved by turning on or off a magnetic field. This is especially convenient in flow systems where other approaches to surface renewal may be difficult or cumbersome. The simplicity and flexibility of this strategy makes it particularly convenient for automated immunoassay methodologies. It is also versatile because a wide choice of immunological reagents can be used.
These two immunosensor systems were applied to the measurement of RIgG using a competitive technique. They were also used in the detection of GaRIgG using a sandwich technique, where peroxidase was the enzyme label for amperometric measurements and urease the label for potentiometric measurements.
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© 1998 B. G. Teubner Verlagsgesellschaft Leipzig
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Santandreu, M., Solé, S., Alegret, S., Martínez-Fàbregas, E. (1998). Immunosensor Systems with Renewable Sensing Surfaces. In: Hock, B., Barceló, D., Cammann, K., Hansen, PD., Turner, A.P.F. (eds) Biosensors for Environmental Diagnostics. Teubner-Reihe UMWELT. Vieweg+Teubner Verlag. https://doi.org/10.1007/978-3-322-93454-3_5
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DOI: https://doi.org/10.1007/978-3-322-93454-3_5
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