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Three-Dimensional Reconstruction of Aperiodic Objects in Electron Microscopy

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Book cover Unconventional Electron Microscopy for Molecular Structure Determination

Abstract

In X-ray crystallography it has become fully evident during the development of protein crystallography that the determination of molecular structures with a thickness of more than ca. 2 nm is not possible by means of unit cell projections since too many atoms overlap. In electron microscopy we face a similar situation. A micrograph, taken at low or medium resolution can be regarded as a projection of the specimen in the direction of the lens axis, since the depth of focus is large compared to the object thickness which is of the order of 10 nm. It is well-known that modern microscopes allow point resolutions better than 0.3 nm. Only in very few cases can images with such a resolution be fully interpreted.

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  1. Abteilung für Strukturforschung I, Max-Planck-Institut für Biochemie, D-8033, Martinsried, W. Germany

    W. Hoppe & D. Typke

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  1. W. Hoppe
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W. Hoppe R. Mason

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© 1979 Friedr. Vieweg & Sohn Verlagsgesellschaft mbH, Braunschweig

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Hoppe, W., Typke, D. (1979). Three-Dimensional Reconstruction of Aperiodic Objects in Electron Microscopy. In: Hoppe, W., Mason, R. (eds) Unconventional Electron Microscopy for Molecular Structure Determination. Advances in Structure Research by Diffraction Methods / Fortschritte der Strukturforschung mit Beugungsmethoden. Vieweg+Teubner Verlag, Wiesbaden. https://doi.org/10.1007/978-3-322-86362-1_7

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  • DOI: https://doi.org/10.1007/978-3-322-86362-1_7

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