Abstract
The velocity of the electroosmotic flow (EOF) is a crucial parameter for separations by capillary electrophoresis. It determines the range of analyte mobilities that can be handled in a single run as well as the run time. Moreover, variations in the EOF, between different capillaries and between different runs on the same capillary, are the main source of the irrepeatability and irreproducibility of the elution times so often encountered in CE. The electroosmotic mobility is in principle governed by the chemical condition of the inner surface of the capillary and by the composition of the background electrolyte (BGE) used for the separation. Still, even with careful control of these parameters, large variations in the EOF are found in practice.
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References
A.J. Bard and L.R. Faulkner, “Electrochemical Methods, Fundamentals and Applications”, J. Wiley & Sons, New York, 1980, Chapter 12.
I. Fried, “The Chemistry of Electrode Processes”; Academic Press; London, 1973; Chapter 4.
R.J. Hunter, “Foundations of Colloid Science”, Clarendon Press; Oxford, 1987; Chapter 6.
J.A. van Lier, P.L. de Bruyn and J. Th.G. Overbeek, J. Phys. Chem. 64 (1960) 1675.
L. Lyklema, J. Electroanal. Chem., 18 (1968) 341.
D.E. Yates, S. Levine and T.W. Healy, Trans. Faraday Soc. 70 (1974) 1807.
J.A. Davis, R.O. James and J.O. Leckie, J. Colloid Interface Sci. 69 (1978) 480.
H. Poppe, A. Cimentes and W. Th. Kok, Anal. Chem., 68 (1996) 888.
S. Kaupp, R. Steffen and H. Waetzig, J. Chromatogr. A, 744 (1996) 93.
T.L. Huang, Chromatographia, 35 (1993) 395.
J. Kohr and H. Engelhardt, J. Microcolumn Sep., 3 (1991) 491.
M.F.M. Tavares and V.L. McGuffin, Anal. Chem., 67 (1995) 3687.
J.E. Dickens, J. Gorse, J.A. Everhart and M. Ryan, J. Chromatogr. B, 657 (1994) 401.
M. Mammen, J. Carbeck, E.E. Simanek and G.M. Whitesides, J. Am. Chem. Soc., 119 (1997) 3469.
C. Schwer and E. Kenndler, Anal. Chem., 63 (1991) 1801.
W.R. Jones and P. Jandik, J. Chromatogr., 608 (1992) 385.
J.C. Reijenga, G.U.A. Aben, T.P.E.M. Verheggen and F.M. Everaerts, J. Chromatogr., 260 (1983) 241.
W.R. Jones and J.P. Jandik, J. Chromatogr., 546 (1991) 445.
C.A. Lucy and R.S. Underhill, Anal. Chem., 68 (1996) 300.
A. Emmer, M. Jansson and J. Roeraade, J. Chromatogr., 547 (1991) 544.
K.K.-C. Yeung and C.A. Lucy, Anal. Chem., 69 (1997) 3435.
M. Chen and R.M. Cassidy, J. Chromatogr., 602 (1992) 227.
A. Dougherty, C. Wooley, D. Williams, D. Swaile, R. Cole and M. Sepaniak, J. Liq. Chromatogr., 14 (1991) 907.
G.J.M. Bruin, R. Huisden, J.C. Kraak and H. Poppe, J. Chromatogr., 480 (1989) 339.
S. Hjerten and K. Kubo, Electrophoresis, 14 (1993) 390.
H. Engelhardt and M.A. Cunat-Walter, J. Chromatogr., 716 (1995) 27.
M. Gilges, M.H. Kleemiss, G. Schomburg, Anal. Chem., 66 (1994) 2038.
H. Bayer and H. Engelhardt, J. Microcolumn Sep., 8 (1996) 479.
M.W.F. Nielen, J. High Resolut. Chromatogr., 16 (1993) 62.
C.S. Lee, W.C. Blanchard, C.-T. Wu, Anal. Chem. 63 (1990) 1550.
M.A. Hayes and A.G. Ewing, Anal. Chem. 64 (1992) 512.
C.A. Keely, R.R. Holloway;, T.A.A.M. van de Goor and D. McManigill, J. Chromatogr.A, 652 (1993) 283.
C.S. Lee, C.-T. Wu, T. Lopes and B. Patel, J. Chromatogr., 559 (1991) 133.
M.S. Bello, L. Capelli and P.G. Righetti, J. Chromatogr.A 684 (1994) 311
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© 2000 Friedr. Vieweg & Sohn Verlagsgesellschaft mbH
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Kok, W. (2000). Capillaries and the Electroosmotic Flow. In: Capillary Electrophoresis: Instrumentation and Operation. Chromatographia CE-Series, vol 4. Vieweg+Teubner Verlag, Wiesbaden. https://doi.org/10.1007/978-3-322-83133-0_6
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DOI: https://doi.org/10.1007/978-3-322-83133-0_6
Publisher Name: Vieweg+Teubner Verlag, Wiesbaden
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