Abstract
For HPLC it has amply been shown that the measurement of fluorescence is one of the most sensitive techniques for detection available. Limits of detection with fluorescence are often two to three orders of magnitude lower than with UV absorbance detection. Since in CE with its small dimensions it is to be expected that detection sensitivity may be problematic for many applications, especially for trace analysis, it seems obvious to develop or modify a fluorescence detector for use in CE.
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References
J.W. Jorgenson and K.D. Lukacs, Anal. Chem., 53 (1981) 1298.
R.G. Brownlee and S. W. Compton, Am. Lab., 20 (1988) 10.
R.H. Zhu and W. Th. Kok, J. Chromatogr. A, 716 (1995) 123.
R.O. Cole, D.L. Hiller, C.A. Chowojdak, and M.J. Sepaniak, J. Chromatogr.A, 736 (1996) 239.
J. Caslavska, E. Gassmann, and W. Thormann, J. Chromatogr. A, 709 (1995) 147.
L. Hernandez, J. Escalona, N. Joshi, and N. Guzman, J. Chromatogr., 559 (1991) 183.
Y.-F. Cheng and N.J. Dovichi, Science, 242 (1988) 562.
S. Wu and N.J. Dovichi, J. Chromatogr., 480 (1989) 141.
E. Arriaga, D.Y. Chen, X.L. Cheng, and N.J. Dovichi, J. Chromatogr. A, 652 (1993) 347.
Application Note nr. 1, Flux Instruments, Pfaffenheim, Germany.
Y.F. Cheng, R.D. Piccard, and T. Vo-Dinh, Appl. Spectrosc., 44 (1990) 755.
J.V. Sweedler, J.B. Shear, H.A. Fishman, R.N. Zare, and R.H. Scheller, Anal. Chem., 63 (1991) 496.
S. Carson, A.S. Cohen, A. Belenkii, M.C. Ruiz-Martinez, J. Berka, and B.L. Karger, Anal. Chem., 65 (1993) 3219.
E. Gassman, J.E. Kuo and R.N. Zare, Science, 230 (1985) 813.
D.E. Burton, M.J. Sepaniak and M.P. Maskarinec, J. Chromatogr. Sci., 24 (1986) 347.
E.J. Guthrie, J.W. Jorgenson and P.L. Dluzneski, J. Chromatogr. Sci., 22 (1984) 171.
D.Y. Chen, K. Adelhelm, X.L. Cheng, and N.J. Dovichi, Analyst, 119 (1994) 349.
A.T. Timperman, K. Khatib, and J.V. Sweedler, Anal. Chem., 67 (1995) 139.
D.Y. Chen and N.J. Dovichi, Anal. Chem., 68 (1996) 690.
A.J.G. Mank, H. Lingeman, and C. Gooijer, Trends Anal. Chem., 15 (1996) 1.
A.J.G. Mank and E.S. Yeung, J. Chromatogr. A, 708 (1995) 309.
B. Nickerson and J.W. Jorgenson, HRC J. High Resolut. Chrom., 11 (1988) 533.
S.D. Gilman and A.G. Ewing, Anal. Chem., 67 (1995) 58.
S.D. Gilman and A.G. Ewing, Anal. Methods Instr., 2 (1995) 133.
A. Guttman, F.T.A. Chen, R.A. Evangelista, and N. Cooke, Anal. Biochem., 233 (1996) 234.
D.J. Rose, Jr., J. Chromatogr., 540 (1991) 343.
D.J. Rose, Jr. and J.W. Jorgenson, J. Chromatogr., 447 (1988) 117.
B. Nickerson and J.W. Jorgenson, J. Chromatogr., 480 (1989) 157.
S.L. Pentoney, Jr., X.H. Huang, D.S. Burgi and R.N. Zare, Anal. Chem., 60 (1988) 2625.
M. Albin, R. Weinberger, E. Sapp, and S. Moring, Anal. Chem., 63 (1991) 417.
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Kok, W. (2000). Fluorescence Detection. In: Capillary Electrophoresis: Instrumentation and Operation. Chromatographia CE-Series, vol 4. Vieweg+Teubner Verlag, Wiesbaden. https://doi.org/10.1007/978-3-322-83133-0_10
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DOI: https://doi.org/10.1007/978-3-322-83133-0_10
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