Summary
To study the dynamic of bacterial soil population it is necessary to detect and quantify different species and strains. The last hundred years Rhizobium are released in the environment to enhance nitrogen fíxation, but until now nothing is known about any ecological effect on microbial communities. Identification of possible effects requires quantitative and qualitative analysis of the bacterial soil population.
Our aim was to test species-specific genes especially of Rhizobium useful for their direct identification and quantifícation in soil. Based on the host-specifity genes nodH of R. meliloti and nodO of R. leguminosarum bv. viciae, respectively, DNA probes have been constructed by PCR amplification. After optimizing colony hybridization techniques both probes have been applied to detect and quantify Rhizobium in two different soils. The estimated amount of nodulating Rhizobium determined by MPN tests has been compared with average bacteria numbers determined by colony hybridizations. During rehybridizations we could find soil bacteria showing a positive signal with both gene probes. The amount of those nodH +/O + colonies, which has been analysed by ARDRA, depended on soil type used for the study. In summary the selected genes are in fact useful to quantify R. meliloti and R. leguminosarum bv. viciae in different soils, but the amount of nodH +/O + colonies has to be determined by rehybridization experiments.
Konzipierung und Durchführung der Arbeiten: Institut für Ökophysiologie der Primärproduktion des ZALF Müncheberg
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Literatur
BERINGER, J.E. (1974). R factor transfer in R. leguminosarum. J. Gen. Microbiol. 84: 188–198
BROMFIELD, E.S.P., WHEATCROFT, R. and BARRAN, L.R. (1994). Medium for direct isolation of Rhizobium meliloti from soils. Soil Biol. Bioehem. 26: 423–428
DEBELLÉ, F., ROSENBERG, C, VASSE I, MAILLET, F., MARTINEZ, E., DÉNARIE, I, and TRUCHET, G. (1986). Assignment of symbiotic developmental phenotypes to common and specifíc nodulation (nod) genetic loci of Rhizobium meliloti. J. Bacteriol. 168: 1075–1086
DeMAAGD, R., WIJFJES, H., SPAINK, H., RUIZ-SAINTZ, J., WIJFFELMAN, C, OKKER, R., and LUGTENBERG, B. (1989). NodO ,a new nod gene of the Rhizobium leguminosarum bv. viciae Sym plasmid encodes a secreted protein. J. Bacteriol. 171: 6764–6770
DOWNIE, J.A., HOMBRECHER, G., MA Q.-S., KNIGHT, CD., WELLS, B., and JOHNSTON, A.W.B. (1983). Cloned nodulation genes of Rhizobium leguminosarum determine host-range specifícity. Mol. Gen. Genet. 190: 359–365
ECONOMOU, A., HAMILTON W., JOHNSTON, A., and DOWNIE, A. (1990). The Rhizobium nodulation gene nodO eneodes a Ca2+ -binding protein that is exported through without N-terminal cleavage and is homologous to haemolysin and related proteins. EMBO J. 9: 349–334
GASSEN, H.G., SACHSE, G.E. and SCHULTE, A. (1994). PCR-Grundlaeen und Anwendung der Polymerase-Kettenreaktion. Gustav Fischer Verlag Stuttgart. ISBN 3-437-20509-9.
HIGASHI, S. (1993). (Brady)Rhizobium-plant Communications involved in infection and nodulation. J. Plant Res. 106:201–211
LAGUERRE, G., BARDIN, M. and AMAGER, N. (1993). Isolation from soil of symbiotic and nonsymbiotic R. leguminosarum by DNA hybridization. Can. J. Microbiol. 39: 1142–1149
SAMBROOK, J., FRITSCH, E.F. and MANIATIS, T. (1989). Molecular cloning: A Laboratory Manual, second edition, CSH Laboratory N.Y.
SELBITSCHKA, W., PÜHLER, A., and SIMON, R. (1992). Bioluminescent RecA mutants of Rhizobium as modeil organisms in risk assessment studies. In: Release of Genetically Modified Microorgamisms-REGEM 2. Eds. D.S. Stewart-Tull and M. Sussman: 225–227
SELENSKA-POBELL, S. (1994). How to monitor released rhizobia. Plant and Soil 166: 187–191
SOMASEGARAN, P. and HOBEN, H.J. (1994). Handbook for Rhizobia. Gustav Springer Verlag Berlin ISBN 0-387-94134: 58–64
STREIT, W., BJOURSON, A., COPPER, J., and WERNER, D. (1993). Application of substraction hybridization for the development of a Rhizobium leguminosarum biovar phaseoli and Rhizobium tropici group specific DNA probe. FEMS Microbiol.Ecol. 13: 59–68
TAS, É, KAUALAINEN, S., SAANO, A, and LINDSTRÖM, K. (1994). Isolation of a Rhizobium galegae strain-specific DNA probe. Microb. Releases 2: 231–237
TICHY, H.-V. und SIMON, R. (1994). Effiziente Analyse von Mikroorganismen mit PCR-Fingerprint-Verfahren. BIOforum 12: 499–505
VINCENT, J.M. (1970).A Manual for the practical study of root-nodule bacteria (IBP Handbook 15) Blackwell Scientific Publications, LTD Oxford
VAN RHIJN, P. and VANDERLEYDEN, J. (1995). The Rhizobium-Plant Symbiosis. Microbiol. Reviews 59: 124–142
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© 1996 B. G. Teubner Verlagsgesellschaft Leipzig
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Labes, G., Lentzsch, P. (1996). Selektion und Anwendung von Wirtsspezifitäts-Genen zum Nachweis von Rhizobium-Spezies im Boden. In: Merbach, W. (eds) Pflanzliche Stoffaufnahme und mikrobielle Wechselwirkungen in der Rhizosphäre. Vieweg+Teubner Verlag. https://doi.org/10.1007/978-3-322-81041-0_3
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DOI: https://doi.org/10.1007/978-3-322-81041-0_3
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