Summary
The associative Rhizobium leguminosarum bv. trifolii strain R39, isolated from red clover nodules, and a corresponding rifampicin resistant, selected mutant R39rif were tested for rhizosphere colonization (rhizosphere soil, washed root, endorhizosphere) with different crops (Pisum sativum, Lupinus albus, Sorghum triticale, Zea mays) in greenhouse experiments using a strain-specific purifíed monospecific polyclonal antiserum and a sensitive chemoluminescence immunoassay in comparision to the common selection-agar technique. There was a good correlation between the two used strains and the two detection methodes. The Rhizobium-strain R39 was able to colonize different legumes and nonleguminous plants associatively very effectively. Quantified 8 weeks after inoculation, under non sterile greenhouse conditions, about 1 % of the total bacterial population in the rhizosphere of the inoculated plants was identified as Rhizobium-strmn R39. Furthermore the strain was detected in the root tissue of all inoculated plants. In a long term experiment the colonization of Zea mays was studied in more detail. The microbial wild population was nearly completely replaced by strain R39 during the vegetative plant development. This indicates a microbial succession process especially in the endorhizosphere.
Frühere Arbeitsstelle und Durchführung der Arbeiten im Institut für Ökophysiologie der Primärproduktion des ZALF Müncheberg
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© 1996 B. G. Teubner Verlagsgesellschaft Leipzig
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Wiehe, W., Schloter, M. (1996). Detektion Eines Rhizosphären-Inokulums Mit Hilfe Antibiotikaresistenter Mutanten und Immunologischer Methoden. In: Merbach, W. (eds) Pflanzliche Stoffaufnahme und mikrobielle Wechselwirkungen in der Rhizosphäre. Vieweg+Teubner Verlag. https://doi.org/10.1007/978-3-322-81041-0_2
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DOI: https://doi.org/10.1007/978-3-322-81041-0_2
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