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Kultivierungsunabhängige Analyse des Einflusses von Chlorpyrifos auf die mikrobielle Lebensgemeinschaft einer Ackerbrache

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Wurzelinduzierte Bodenvorgänge

Abstract

We investigated the effect of Chlorpyrifos on a bacterial community. In accordance with results of other groups treatment with Chlorpyrifos in normal concentrations seems to be without influence on the majority of bacteria. But in contrast to previous results we found by SSCP-fingerprinting that few bacteria among Arthrobacter and Klebsiella/Serratia were influenced over few months. The sequence behind the increased bands 1 and 2 in two Arthrobacter-fingerprints (Abb. 1) matched Arthrobacter sulfureus with a similarity of 100%. The decreased band 1 of Klebsiella/Serratia-fingerprints (Abb. 2) in summer was identified as an uncultured agricultural soil bacterium. The increased band 2 seems to be another uncultured bacterium. No shifts were found for Pseudomonas and Streptomyces. So it seems that some species benefited from this treatment whereas other species were inhibited. Now the following question arises. Are direct or indirect influences on soil bacteria responsible for changes in the community¿

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Literaturverzeichnis

  • Abdel-Kader; M.I.A. Moubasher A.H.; Abdel-Hafez S.I., 1978: Selective effect of five pesticides on soil and cotton-rhizosphere and -rhizoplane fungus flora. Mycopath. 84, 151–158

    Article  Google Scholar 

  • Lane, D.J., 1991: 16S/23S rRNA sequencing. In: Stackebrandt, E. and M. Goodfellow (eds.) John Wiley ad Son: Nucleic Acid Techniques in Bacterial Systematics., New York, N.Y. 115–175.

    Google Scholar 

  • Lieber, A.; Kiesel, B.; Babe, L W., 2003: Microbial diversity analysis of soil by SSCP fingerprinting technique using TGGE Maxi System. In: Merbach, W.; Hütsch, B.W.; Augustin, J. (Hrsg.): Prozessregulation in der Rhizosphäre. Leipzig, B. G. Teubner, 61–65

    Google Scholar 

  • Moawad, H.A.A.; ZohdyL.I; Badr El-Din S.M.S.; Gamal El-Din H. 1979: Changes in certein biological and chemical properties of the urea treated soil as affected by some pesticides application. Egypt. J. Microbiol. 14, 29–36

    CAS  Google Scholar 

  • Sambrook, J; Fritsch, E.F.; Maniahs, T., 1989: Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Press

    Google Scholar 

  • Schädler, M., 2001: Einfluss phytophager Insekten auf die Struktur und Dynamik einer Ackerbrache (Dissertation). — UFZ — Berichte 11/2001, 1–111.

    Google Scholar 

  • Schwieger, F.; Tebbe, C.C., 1998: A New Approach To Utilize PCR-Single-Strand-Conformation Polymorphism for 16S rRNA Gene-Based Microbial Community Analysis. Applied and Environmental Microbiology 64, 4870–4876

    PubMed  CAS  Google Scholar 

  • Sivasithamparam, K., 1969: Some effects of an insecticide (‘Dursban’) and a weedicide (‘Linuron’) on the microflora of a submerged soil. Proc. Ceylon Ass. Advmt. Sci. 25, 1–8

    Google Scholar 

  • Tu, C.M, 1978: Effect of pesticides on acetylene reduction and microorganisms in a sandy loam. Soil Biology and Biochemistry 10, 451–456

    Article  CAS  Google Scholar 

  • Tu, C.M., 1980: Influence of five pyrethroid insecticides on microbial populations and activities in soil. Microbial Ecoogy. 5, 321–327

    Article  CAS  Google Scholar 

  • Tu, C.M., 1981: Effects of pesticides on activities of enzymes and microorganisms in a clay soil. Journal of Environmental Science and Health B 16, 179–191

    Article  CAS  Google Scholar 

  • Weiiington, E.M.; Stackebrandt, E.; Sanders, D.; Wolstrup, J.; Jorgensen, N.O., 1992: Taxonomic status of Kitasatosporia, and proposed unification with Streptomyces on the basis of phenotype and 16S rRNA analysis and emendation of Streptomyces Waksman and Henrici 1943, 339AL. International Journal of Systematic Bacteriology 42, 156–160

    Article  Google Scholar 

  • Widmer, F.; Seidler, R.J.; Gillevet, P.M.; Watrud, LS.; Di Giovanni, G.D., 1998: A highly seletive PCR protocol for detecting 16S rRNA genes of the genus Pseudomonas (sensu stricto) in environmental samples. Applied and Environmental Microbiology 64, 2545–2553

    PubMed  CAS  Google Scholar 

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© 2004 B. G. Teubner Verlag / GWV Fachverlage GmbH, Wiesbaden

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Lieber, A., Kiesel, B., Babel, W. (2004). Kultivierungsunabhängige Analyse des Einflusses von Chlorpyrifos auf die mikrobielle Lebensgemeinschaft einer Ackerbrache. In: Merbach, W., Egle, K., Augustin, J. (eds) Wurzelinduzierte Bodenvorgänge. Vieweg+Teubner Verlag. https://doi.org/10.1007/978-3-322-80084-8_7

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  • DOI: https://doi.org/10.1007/978-3-322-80084-8_7

  • Publisher Name: Vieweg+Teubner Verlag

  • Print ISBN: 978-3-519-00516-2

  • Online ISBN: 978-3-322-80084-8

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