Zum Abbau aromatischer Xenobiotika im Wurzelraum von Helophyten und zur Beeinflussung der Bakterienzönose in der Rhizosphäre durch Schadstoffexposition

Abstract

Degradation of 2,6-dimethylphenol, 4-nitrophenol and naphthalene, which are potential aromatic contaminants of the environment was investigated in the root sphere of helophytes applying both batch- and continuous culture conditions. The degradation processes were performed with hydroponic or sand-bed cultures. The influence of these xenobiotics were studied on the bacteria present in the rhizosphere, both in roots as well as in sand. Total cell numbers, colony forming units (CFUs) and the taxonomic classification of some degraders were also determined. Illustrating the changes in the composition of the bacterial communities, PCR-fingerprint techniques were applied. The colonization of roots and sand particles by bacteria was visualized by the confocal laser scanning microscopy (CLSM).