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Blur Restoration of Confocal Microscopy with Depth and Horizontal Dependent PSF

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Distributed Computing and Artificial Intelligence, Special Sessions, 15th International Conference (DCAI 2018)

Part of the book series: Advances in Intelligent Systems and Computing ((AISC,volume 801))

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Abstract

Confocal microscopy is a popular technique for 3D imaging of biological specimens. Confocal microscopy images are degraded by residual out of focus light. Several restoration methods have been proposed to reduce these degradations. The major one is Richardson Lucy based deconvolution (RL). Even when employing this method images are still blurry. This is mainly caused due to spherical aberration that depends on the distance from lens. Hence, in the previous study, the restoration method is taking only the depth direction into account. In this paper, predicting PSF more correctly, an image restoring method using RL method and Point Spread Function that is considered based on the depth and horizontal effect of direction, is proposed.

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References

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Authors and Affiliations

  1. Osaka Prefecture University, Sakai, Japan

    Yuichi Morioka, Katsufumi Inoue & Michifumi Yoshioka

  2. Hiroshima Institute of Technology, Hiroshima, Japan

    Masaru Teranishi

  3. Juntendo University, Tokyo, Japan

    Takashi Murayama

Authors
  1. Yuichi Morioka
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  2. Katsufumi Inoue
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  3. Michifumi Yoshioka
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  4. Masaru Teranishi
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  5. Takashi Murayama
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Corresponding author

Correspondence to Yuichi Morioka .

Editor information

Editors and Affiliations

  1. BISITE Digital Innovation Hub, University of Salamanca, Salamanca, Spain

    Sara Rodríguez

  2. BISITE Digital Innovation Hub, University of Salamanca, Salamanca, Spain

    Javier Prieto

  3. GECAD - Instituto Superior de Engenharia, Porto, Portugal

    Pedro Faria

  4. Department of Computer Science and Production Management, University of Zielona Góra, Zielona Góra, Poland

    SÅ‚awomir KÅ‚os

  5. Computing Science and Artificial Intelligence, Rey Juan Carlos University, Móstoles, Madrid, Spain

    Alberto Fernández

  6. Basque Center for Applied Mathematics, Bilbao, Spain

    Santiago Mazuelas

  7. Basque Center for Applied Mathematics, Universidad de Alcalá, Alcalá de Henares, Spain

    M. Dolores Jiménez-López

  8. Departamento de Informática y Automática, University of Salamanca, Salamanca, Spain

    María N. Moreno

  9. Departamento de Sistemas Informáticos, University of Castilla-La Mancha, Albacete, Spain

    Elena M. Navarro

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Morioka, Y., Inoue, K., Yoshioka, M., Teranishi, M., Murayama, T. (2019). Blur Restoration of Confocal Microscopy with Depth and Horizontal Dependent PSF. In: Rodríguez, S., et al. Distributed Computing and Artificial Intelligence, Special Sessions, 15th International Conference. DCAI 2018. Advances in Intelligent Systems and Computing, vol 801. Springer, Cham. https://doi.org/10.1007/978-3-319-99608-0_19

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