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Acetylcholinesterase (AChE) Biosensors for Determination of Carbamate Pesticides

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Abstract

Carbamate pesticides are widely applied for controlling pests in agriculture crops due to high insecticidal action and persistence in the environment. The wide applications of carbamate pesticide has required for strictly monitoring the residue levels of carbamate pesticide. Currently several analytical techniques are available for determination of carbamate pesticide residues such as gas chromatography, high-performance liquid chromatography coupled with various detectors, ultraviolet spectroscopy, surface plasmon resonance and fluorimetry involved a sample preparation step prior to further analysis. However, these techniques have some drawbacks such as requiring skill work force and time-consuming sample extraction procedures with high volumes of organic solvents. Enzymatic biosensors-based acetylcholinesterase offers a simple, rapid, high sensitivity and on-site monitoring for determination of carbamate pesticide concentrations. In this book chapter , enzyme-based biosensor methods briefly explained for determination of carbamate pesticide levels, and the immobilization techniques and carbamate pesticide toxicity discussed.

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Acknowledgement

This work was supported by grants from the Ministry of Education Malaysia, Fundamental Research Grant Scheme (FRGS) (No. FRGS/1/2014/SG05/UMS/02/4).

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  1. Biotechnology Research Institute, Universiti Malaysia Sabah, Kota Kinabalu, Sabah, Malaysia

    Anwar Samsidar & Shafiquzzaman Siddiquee

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  1. Biotechnology Research Institute, Universiti Malaysia Sabah, Kota Kinabalu, Sabah, Malaysia

    Shafiquzzaman Siddiquee

  2. Material and Mineral Research Unit (MMRU), Faculty of Engineering, Universiti Malaysia Sabah, Jalan UMS, Kota Kinabalu, Sabah, Malaysia

    Gan Jet Hong Melvin

  3. Faculty of Engineering, Energy Research Unit (ERU), Universiti Malaysia, Kota Kinabalu, Sabah, Malaysia

    Md. Mizanur Rahman

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Samsidar, A., Siddiquee, S. (2019). Acetylcholinesterase (AChE) Biosensors for Determination of Carbamate Pesticides. In: Siddiquee, S., Melvin, G., Rahman, M. (eds) Nanotechnology: Applications in Energy, Drug and Food. Springer, Cham. https://doi.org/10.1007/978-3-319-99602-8_18

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