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Performance Enhancement of Polypyrrole Based Nano-Biosensors by Different Enzyme Deposition Techniques

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Modern Sensing Technologies

Part of the book series: Smart Sensors, Measurement and Instrumentation ((SSMI,volume 29))

Abstract

This chapter presents a comparison of results from a range of experiments carried out to investigate the performance dependency of polypyrrole based nano-biosensors on fabrication and enzyme immobilization techniques. The methods compared are drop casting, co-entrapment, and electrophoretic enzyme deposition techniques. Templated polypyrrole nanotube array sensors provided high sensitivities and quick response times. The size of the template pore diameter plays a vital role in enzyme immobilizing in terms of loading capacity. Application of a polymer cross linking agent provided enhanced sensitivities. However, the response time of the crosslinked sensor was little longer than that of the physical adsorption type enzyme loading. The templated nanostructures have recorded a glucose measurement sensitivity of 62.5 mAM−1 cm−2 with a response time of 6 s. In contrast to the templated method nano-corrugated polypyrrole structures were fabricated on Indium Tin Oxide planar surface using pulse deposition method. The electrochemically developed nano-corrugated polypyrrole sensors with enzyme loaded under high electric field of 1 kV/m have displayed an extremely high sensitivity of 325 mAM−1 cm−2. The study shows the enzyme immobilizing techniques play a great role in the conducting polymer based nano-biosensor performance.

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References

  1. N. Hui et al., Nickel nanoparticles modified conducting polymer composite of reduced graphene oxide doped poly(3,4-ethylenedioxythiophene) for enhanced nonenzymatic glucose sensing. Sens. Actuators B Chem. 221, 606–613 (2015)

    Article  Google Scholar 

  2. T.A. Rajesha, D. Kumar, Recent progress in the development of nano-structured conducting polymers/nanocomposites for sensor applications. Sens. Actuators B Chem. 136, 275–286 (2009)

    Article  Google Scholar 

  3. J.D. Flower et al., Practical chemical sensors from chemically derived graphene. ACS Nano 3(2), 301–306 (2009)

    Article  MathSciNet  Google Scholar 

  4. X.-W. Liu et al., Fabrication of metal-graphene hybrid materials by electroless deposition. Carbon 49(2), 477–483 (2011)

    Article  Google Scholar 

  5. A. Safavi, N. Maleki, E. Farjami, Fabrication of a glucose sensor based on a novel nanocomposite electrode. Biosens. Bioelectron. 24(6), 1655–1660 (2009)

    Article  Google Scholar 

  6. Y. Fan et al., Graphene–polyaniline composite film modified electrode for voltammetric determination of 4-aminophenol. Sens. Actuators B Chem. 157(1), 669–674 (2011)

    Article  Google Scholar 

  7. D.J. Macaya et al., Simple glucose sensors with micromolar sensitivity based on organic electrochemical transistors. Sens. Actuators B Chem. 123(1), 374–378 (2007)

    Article  Google Scholar 

  8. J. Heinze, B.A. Frontana-Uribe, S. Ludwigs, Electrochemistry of conducting polymers persistent models and new concepts. Chem. Rev. 110, 4724–4771

    Google Scholar 

  9. C. Liu, T. Kuwahara, R. Yamazaki, M. Shimomura, Covalent immobilization of glucose oxidase on films prepared by electrochemical copolymerization of 3-methylthiophene and thiophene-3-acetic acid for amperometric sensing of glucose: effects of polymerization conditions on sensing properties. Eur. Polym. J. 43, 3264–3276 (2007)

    Article  Google Scholar 

  10. M. Braik et al., Highly sensitive amperometric enzyme biosensor for detection of superoxide based on conducting polymer/CNT modified electrodes and superoxide dismutase. Sens. Actuators B Chem. 236, 574–582 (2016)

    Article  Google Scholar 

  11. M.M. Barsan, M.E. Ghica, C.M.A. Brett, Electrochemical sensors and biosensorsbased on redox polymer/carbon nanotube modified electrodes: a review. Anal. Chim. Acta 881, 1–23 (2015)

    Article  Google Scholar 

  12. V. Pifferi et al., Synthesischaracterization and influence of poly (brilliant green) on the performance of different electrode architectures based on carbon nanotubes andpoly(3,4-ethylenedioxythiophene). Electrochim. Acta 98, 199–207 (2013)

    Article  Google Scholar 

  13. D. Restuccia et al., New EU regulation aspects and global market of active and intelligent packaging for food industry applications. Food Control 21(11), 1425–1435 (2010)

    Article  Google Scholar 

  14. M. Singh, P.K. Kathuroju, N. Jampana, Polypyrrole based ampherometric glucose biosensors. Sens. Actuators B Chem. 143(1), 430–443 (2009)

    Google Scholar 

  15. E.M.I. Mala Ekanayake, D.M.G. Preethichandra, K. Kaneto, Polypyrrole nanotube array sensor for enhanced adsorption of glucose oxidase in glucose biosensors. Biosens. Bioelectron. 23(1), 107–113 (2007)

    Google Scholar 

  16. R. Brooke et al., Recent advances in the synthesis of conducting polymers from the vapour phase. Prog. Mater Sci. 86, 127–146 (2017)

    Article  Google Scholar 

  17. E.M.I. Mala Ekanayake, D.M.G. Preethichandra, K. Kaneto, Effect of glucose oxidase immobilizing techniques on performances of nano scale polypyrrole glucose biosensors. Jpn. J. Appl. Phys. 47(2), PART 2, 1321–1324 (2008)

    Google Scholar 

  18. V. Manzeiko et al., Gold nanoparticle and conducting polymer-polyaniline-based nanocomposites for glucose biosensor design. Sens. Actuator B Chem. 189, 187–193 (2013)

    Article  Google Scholar 

  19. J. Liu, M. Agarwal, K. Varahramyan, Glucose sensor based on organic thin film transistor using glucose oxidase and conducting polymer. Sens. Actuator B Chem. 135, 195–199 (2008)

    Article  Google Scholar 

  20. P.A. Palod, V. Singh, Improvement in glucose biosensing response of electrochemically grown polypyrrole nanotubes by incorporating crosslinked glucose oxidase. Mat. Sci. Eng. C 55, 420–430 (2015)

    Google Scholar 

  21. H. Azak et al., Electrochemical glucose biosensing via new generation DTP type conducting polymers/gold nanoparticles/glucose oxidase modified electrodes. J. Electroanal. Chem. 782, 138–153 (2016)

    Article  Google Scholar 

  22. T.C. Gokoglan et.al., A novel approach for the fabrication of a flexible glucose biosensor: the combination of vertically aligned CNTs and a conjugated polymer. Food Chem. 220, 299–305 (2017)

    Google Scholar 

  23. A.A. Saei et. al. Electrochemical biosensors for glucose based on metal nanoparticles. Talanta 130, 198–206 (2014)

    Google Scholar 

  24. J.R. Anusha et., al., Simple fabrication of ZnO/Pt/chitosan electrode for enzymatic glucose biosensor. Sens. Actuators B Chem. 202, 827–833 (2014)

    Google Scholar 

  25. https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Datasheet/7/g2133dat.pdf. Accessed on 4 May 2017

  26. D.M.G. Preethichandra and E.M.I. Mala Ekanayake, Nano-biosensor development for biomedical and environmental measurements, in New Developments and Applications in Sensing Technology, ed. by S.C. Mukhopadhyay et al., LNEE 83, pp. 279–292

    Google Scholar 

  27. D.M.G. Preethichandra, E.M.I. Mala Ekanayake, K. Kaneto, Development of low-cost nano-biosensors to enhance the rural healthcare services, in IEEE 6th International Conference on Sensing Technology, ICST2012, Kolkata, India, 18 Dec 2012, pp. 163–166

    Google Scholar 

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Authors and Affiliations

  1. School of Engineering and Technology, Central Queensland University, Rockhampton, Australia

    D. M. G. Preethichandra & E. M. I. Mala Ekanayake

  2. Graduate School of Engineering, University of Hyogo, Himeji, Hyogo, Japan

    M. Onoda

  3. Department of Biomedical Engineering, Osaka Institute of Technology, Osaka, Japan

    K. Kaneto

Authors
  1. D. M. G. Preethichandra
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  2. E. M. I. Mala Ekanayake
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  3. M. Onoda
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  4. K. Kaneto
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Corresponding author

Correspondence to D. M. G. Preethichandra .

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Editors and Affiliations

  1. Macquarie University, Sydney, New South Wales, Australia

    Subhas Chandra Mukhopadhyay

  2. Macquarie University, Sydney, New South Wales, Australia

    Krishanthi P. Jayasundera

  3. Instituto de Telecomunicações and ISCTE-IUL, Lisbon, Portugal

    Octavian Adrian Postolache

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Preethichandra, D.M.G., Mala Ekanayake, E.M.I., Onoda, M., Kaneto, K. (2019). Performance Enhancement of Polypyrrole Based Nano-Biosensors by Different Enzyme Deposition Techniques. In: Mukhopadhyay, S., Jayasundera, K., Postolache, O. (eds) Modern Sensing Technologies . Smart Sensors, Measurement and Instrumentation, vol 29. Springer, Cham. https://doi.org/10.1007/978-3-319-99540-3_11

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  • DOI: https://doi.org/10.1007/978-3-319-99540-3_11

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  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-319-99539-7

  • Online ISBN: 978-3-319-99540-3

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