Abstract
Aggregation-induced emission (AIE) luminogens (AIEgens) are emerging as a new class of luminescent materials for bioimaging. They are usually with excellent optical properties, appreciable biocompatibility and thus have been used extensively for cell imaging and sensing by employing conventional fluorescence microscopy and confocal microscopy. Recently, their applications in advanced imaging techniques such as super-resolution imaging, fluorescence lifetime imaging (FLIM), and fluorescence anisotropy imaging (FAIM) have been explored and successfully demonstrated. In this chapter, we select some representative examples to illustrate the explorations and discoveries in this area.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Change history
01 January 2019
The images in this chapter was taken from external sources and the publishers were not acknowledged and that the correction has been carried out. Below is the list of images with their appropriate captions along with the publishers from whom the copyright permissions were obtained.
References
Yu J, Sun X, Cai F et al (2015) Low photobleaching and high emission depletion efficiency: the potential of AIE luminogen as fluorescent probe for STED microscopy. Opt Lett 40:2313. https://doi.org/10.1364/OL.40.002313
Li D, Qin W, Xu B et al (2017) AIE nanoparticles with high stimulated emission depletion efficiency and photobleaching resistance for long-term super-resolution bioimaging. Adv Mater 29:1–9. https://doi.org/10.1002/adma.201703643
Fang X, Chen X, Li R et al (2017) Multicolor photo-crosslinkable aiegens toward compact nanodots for subcellular imaging and sted nanoscopy. Small 13:1–7. https://doi.org/10.1002/smll.201702128
Rust MJ, Bates M, Zhuang X (2006) imaging by stochastic optical reconstruction microscopy (STORM). Nat Methods 3:793–796. https://doi.org/10.1038/NMETH929
Fernández-Suárez M, Ting AY (2008) Fluorescent probes for super-resolution imaging in living cells. Nat Rev Mol Cell Biol 9:929–943. https://doi.org/10.1038/nrm2531
Li C, Gong W-L, Hu Z et al (2013) Photoswitchable aggregation-induced emission of a dithienylethene–tetraphenylethene conjugate for optical memory and super-resolution imaging. RSC Adv 3:8967. https://doi.org/10.1039/c3ra40674a
Gu X, Zhao E, Zhao T et al (2016) A mitochondrion-specific photoactivatable fluorescence turn-on aie-based bioprobe for localization super-resolution microscope. Adv Mater 28(25):5064–5071. https://doi.org/10.1002/adma.201505906
Lo CYW, Chen S, Creed SJ et al (2016) Novel super-resolution capable mitochondrial probe, MitoRed AIE, enables assessment of real-time molecular mitochondrial dynamics. Sci Rep 6:1–9. https://doi.org/10.1038/srep30855
Zhao N, Chen S, Hong Y, Tang BZ (2015) A red emitting mitochondria-targeted AIE probe as an indicator for membrane potential and mouse sperm activity. Chem Commun 51:13599–13602. https://doi.org/10.1039/C5CC04731E
Hong Y, Lam JWY, Tang BZ (2009) Aggregation-induced emission: phenomenon, mechanism and applications. Chem Commun (29):4332–4353. https://doi.org/10.1039/b904665h
Gao M, Hong Y, Chen B et al (2017) AIE conjugated polyelectrolytes based on tetraphenylethene for efficient fluorescence imaging and lifetime imaging of living cells. Polym Chem 8:3862–3866. https://doi.org/10.1039/C7PY00564D
Chen S, Hong Y, Zeng Y et al (2015) Mapping live cell viscosity with an aggregation-induced emission fluorogen by means of two-photon fluorescence lifetime imaging. Chem Eur J 21:4315–4320. https://doi.org/10.1002/chem.201405658
Soleimaninejad H, Chen MZ, Lou X et al (2017) Measuring macromolecular crowding in cells through fluorescence anisotropy imaging with an AIE fluorogen. Chem Commun 53:2874–2877. https://doi.org/10.1039/C6CC09916E
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2019 Springer Nature Switzerland AG
About this chapter
Cite this chapter
Zhao, E., Chen, S. (2019). Applications of AIEgens in Super-Resolution Imaging, Fluorescence Lifetime Imaging, and Fluorescence Anisotropy Imaging. In: Tang, Y., Tang, B. (eds) Principles and Applications of Aggregation-Induced Emission. Springer, Cham. https://doi.org/10.1007/978-3-319-99037-8_17
Download citation
DOI: https://doi.org/10.1007/978-3-319-99037-8_17
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-319-99036-1
Online ISBN: 978-3-319-99037-8
eBook Packages: Chemistry and Materials ScienceChemistry and Material Science (R0)