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Mathematical Modeling of Protein Complexes pp 1–12Cite as

Physical Methods for Studying Proteins

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Part of the book series: Biological and Medical Physics, Biomedical Engineering ((BIOMEDICAL))

Abstract

This chapter discusses various experimental approaches for determining the structure of molecules, the mutual arrangement of domains in space, the identification of active protein centers, and their advantages and limitations in the study of various physical properties of biological complexes.

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References

  1. E. Gaal, G. Medbesi, L. Veretski, Electrophoresis in the Division Biological Macromolecules (Moscow, 1982), 448 pp

    Google Scholar 

  2. V.F. Gavrilenko, M.E. Ladygina, L.M. Khandobina, A Large Practical Workshop on Plant Physiology (Higher School, Moscow, 1975), 327 pp

    Google Scholar 

  3. V.M. Golitsyn, Non-denaturing electrophoresis. Fractionation photosynthetic pigment-protein complexes and plasma proteins. Biochemistry 64, 64–70 (1999)

    Google Scholar 

  4. V.S. Kamyshnikov, Reference Book on Clinical and Biochemical Laboratory Diagnostics (in 2 Volumes) (Minsk, 2000), 463 pp

    Google Scholar 

  5. L.A. Osterman, Methods for Studying Proteins and Nucleic Acids: Electrophoresis and Ultracentrifugation (Nauka, Moscow, 1981), 288 pp

    Google Scholar 

  6. V.I. Safonov, M.P. Safonova, Research of proteins and enzymes of plants by electrophoresis in a polyacrylamide gel. Biochemical Methods in Plant Physiology (Nauka, Moscow, 1971), pp. 113–137

    Google Scholar 

  7. V.N. Titov, V.A. Amelyushkina, Electrophoresis of Proteins Blood Serum (Optium Press, 1994), 62 pp

    Google Scholar 

  8. E. Heftman, T. Caster, A. Niderweiser, N. Katsimpulas, A. Kuksis, R. Krotyau, R. Ronald, in Chromatography. Practical Application of the Method. Part 1, ed. by E. Heftmann (Moscow, 1986), 336 pp

    Google Scholar 

  9. H. Schluter, Reversed-phase chromatography. J. Chromatogr. Libr. 61, 147–234 (2000)

    Article  Google Scholar 

  10. A. Jungbauer, C. Machold, Chromatography of proteins. J. Chromatogr. Libr. 69. Part B, 669–737 (2004)

    Article  Google Scholar 

  11. M. Caude, A. Jardy, Normal-phase liquid chromatography. Chromatogr. Sci. Ser. 78, 325–363 (1998)

    Google Scholar 

  12. M. Rogner, Size exclusion chromatography. J. Chromatogr. Libr. 61, 89–145 (2000)

    Google Scholar 

  13. F. Anspach, Affinity chromatography. J. Chromatogr. Libr. 69. Part, 139–169 (2004)

    Google Scholar 

  14. P. Cuatrecasas, M. Wilchek, Affinity chromatography. Encycl. Biol. Chem. 1, 51–56 (2004)

    Article  Google Scholar 

  15. D. Hage, M. Nelson, Chromatographic immunoassays. Anal. Chem. 73, 198A–205A (2001)

    Article  Google Scholar 

  16. A. Henschen, K. Xyp, F. Lotshpayh, V. Walter, Highly Effective Liquid Chromatography in Biochemistry (Moscow, 1988), 688 pp

    Google Scholar 

  17. L. Osterman, Chromatography of Proteins and Nucleic Acids (Nauka, Moscow, 1985), 536 pp

    Google Scholar 

  18. K. Wilson, J. Walker, Principles and Methods of Biochemistry and Molecular Biology (Binom, Moscow, 2013)

    Google Scholar 

  19. N.A. Klyuev, E.S. Brodsky, Modern methods mass spectrometric analysis of organic compounds. J. Russ. Chem. Soc. named after D.I. Mendeleyev’s XLVI(4) (2002)

    Google Scholar 

  20. R. Aebersold, M. Mann, Mass spectrometry-based proteomics. Nature. 422(6928), 198–207 (2003)

    Article  ADS  Google Scholar 

  21. M. Mann, R. Hendrickson, A. Pandey, Analysis of proteins and proteomes by mass spectrometry. Annu. Rev. Biochem. 70, 437–473 (2001)

    Google Scholar 

  22. J. Fenn, M. Mann, C. Meng, S. Wong, C. Whitehouse, Electrospray ionization for mass spectrometry of large biomolecules. Science 246(4926), 264–271 (1989)

    Article  Google Scholar 

  23. J. Fenn, M. Mann, C. Meng, S. Wong, C. Whitehouse, Electrospray ionization-principles and practice. Mass Spectrom. Rev. 9(1), 37–70 (1990)

    Article  ADS  Google Scholar 

  24. A.T. Lebedev, K.A. Artemenko, TYu. Samghina, Basics Mass Spectrometry of Proteins and Peptides (Technosphere VMSO, Moscow, 2012)

    Google Scholar 

  25. T. Blandel, L. Johnson, Crystallography of Protein (Moscow, 1979)

    Google Scholar 

  26. S. D’Arcy, K.W. Martin, T. Panchenko, X. Chen, S. Bergeron, L.A. Stargell, B.E. Black, K. Luger, Chaperone Nap1 shields histone surfaces used in a nucleosome and can put H2A-H2B in an unconventional tetrameric form. Mol. Cell 51(5), 662–677 (2013)

    Article  Google Scholar 

  27. A.J. Andrews, G. Downing, K. Brown, Y.J. Park, K. Luger, A thermodynamic model for Nap1-histone interactions. J. Biol. Chem. 283(47), 32412–32418 (2008)

    Article  Google Scholar 

  28. C. Aguilar-Gurrieri, Structural evidence for Nap1-dependent H2A-H2B deposition and nucleosome assembly. EMBO J. 35(13), 1465–1482 (2016)

    Google Scholar 

  29. B. Williams, K. Wilson, Methods of Practical Biochemistry (Moscow, 1978)

    Google Scholar 

  30. Workshop on Biochemistry, ed. by S.E. Severin, G.A. Solovyovoy (Moscow State University, Moscow, 1989)

    Google Scholar 

  31. G.D. Fasman, Circular Dichroism and the Conformational Analysis of Biomolecules (Springer, Berlin, 1996)

    Book  Google Scholar 

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Authors and Affiliations

  1. Saint Petersburg State University, Saint Petersburg, Russia

    Tatiana Koshlan

  2. Peter the Great St. Petersburg Polytechnic University, Saint Petersburg, Russia

    Kirill Kulikov

Authors
  1. Tatiana Koshlan
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  2. Kirill Kulikov
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Correspondence to Kirill Kulikov .

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Koshlan, T., Kulikov, K. (2018). Physical Methods for Studying Proteins. In: Mathematical Modeling of Protein Complexes. Biological and Medical Physics, Biomedical Engineering. Springer, Cham. https://doi.org/10.1007/978-3-319-98304-2_1

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