Abstract
In the opening paragraph of this text, we noted that often students and technologists use a confocal microscope to collect images without a full understanding of how the system works. Unfortunately, an additional factor affecting image collection is that often students and technologists are given a preconceived idea of what the final image or data should look like. With the variety of mechanisms available on a confocal system for changing the appearance of an image observed through a microscope, it is essential that the user does not manipulate settings to match expected outcomes concerning their data. This chapter provides 12 guiding principles that need to be addressed when collecting and processing digital images with confocal and other microscopes and a list of resources available for assistance with confocal imaging.
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Additional Literature Cited
Baker H (1742) The Microscope Made Easy; or I. The nature, uses, and magnifying powers of the best kinds of microscopes described… for the instruction of such, particularly, as desire to search into the wonders of the minute creation… II. An account of what surprising discoveries have been already made by the microscope… And also a great variety of new experiments and observations… London, R. Dodsley. 311pp
Cromer DW (2010) Avoiding twisted pixels: ethical guidelines for the appropriate use and manipulation of scientific digital images. Sci Eng Ethics 16:639–667
Goldenring JR (2010) Innocence and due diligence: managing unfounded allegations of scientific misconduct. Acad Med 85(3):527–530
Hwang et al. (2005) Patient specific embryonic stem cells derived from human SCNT blastocysts. Science 308:1777–1783
Krueger J (2005) Confronting image manipulation of digital images in science. ORI Newsletter 13(3):8–9
Krueger J (2009) Incidences of ORI cases involving falsified images. ORI Newsletter 17(4):2–3
North AJ (2006) Seeing is believing? A beginners’ guide to practical pitfalls in image acquisition. J Cell Biol 172(1):9–18
Price RL (2014 Aug) 2014. Editorial: data repositories, meeting proceedings, open access/online publications, and first reporting issues. Microsc Microanal 20(4):996–998. https://doi.org/10.1017/S1431927614012926
Rossner M, Yamada KM (2004) What’s in a picture? The temptation of image manipulation. J Cell Biol 166(1):11–15
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Jerome, W.G., Price, R.L. (2018). Ethics and Resources. In: Jerome, W., Price, R. (eds) Basic Confocal Microscopy. Springer, Cham. https://doi.org/10.1007/978-3-319-97454-5_12
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DOI: https://doi.org/10.1007/978-3-319-97454-5_12
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