Abstract
With the development of the global economy, the demand for castor bean and castor oil is increasing rapidly, but its cultivation suffers from a lack of high-yielding varieties due to limited genetic research. In this chapter, we describe novel SSR markers developed from the castor bean genome sequence and the first SSR-based genetic linkage map of castor bean, constructed with three different F2 populations derived from crosses between the YC2, YF1, and YF2 lines. The SSR density in the castor bean genome is approximately 15.81 SSR/Mbp, and the frequency of SSR motifs decreased with the increase of repeat unit size. Dinucleotide and trinucleotide repeats, with (AT)n and (AAT)n are the most common repeat units. The linkage map consisted of 331 markers, distributed on ten linkage groups (LGs), encompassing 1164.73 cM, with an average marker interval of 3.63 cM. We will also discuss the first high-density genetic map of castor by using SLAF markers, developed by specific length amplified fragment sequencing (SLAF-seq). This map contains 4300 markers as well as 120 SSR markers with an average marker interval of 0.35 cM, making it the densest castor bean genetic map. These genetic resources are expected to facilitate castor bean research and breeding as well as comparative genomics analyses within the spurge family.
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Yin, X., Lu, J., Tomar, R.S., Chauhan, R.S., Agyenim-Boateng, K.G. (2018). Molecular Linkage Maps in Castor Bean. In: Kole, C., Rabinowicz, P. (eds) The Castor Bean Genome. Compendium of Plant Genomes. Springer, Cham. https://doi.org/10.1007/978-3-319-97280-0_5
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DOI: https://doi.org/10.1007/978-3-319-97280-0_5
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