Abstract
Enzyme-linked immunosorbent assay (ELISA) is a method applicable for scientific and applied research in microbiology. In the study we reviewed the design, format, and main and auxiliary components of ELISA test systems and give a basic protocol of ELISA. Polyclonal antibodies (PAbs) to bacterial/yeast antigens are reagents suitable for ELISA. We presented six PAb preparations obtained as a result of rabbit immunization with whole cells of Saccharomyces cerevisiae BIM Y-195, Debaryomyces hansenii BIM Y-4, Bacillus cereus BIM B-491, Lactobacillus plantarum BIM B-495D, Lactococcus lactis subsp. cremoris BIM B-493D, and Bifidobacterium bifidum BIM В-733D. The protocols for rabbit immunization and determination of PAb primary characteristics, viz., antiserum working titer and cross-reactivity with bacteria/yeast related to strain immunogen are given. Two examples of PAb usage are shown: (1) test systems for quantification of bacterial cells in culture media or other fluids and (2) the methodology for studying the dynamics of synthesis and secretion of bacterial antigens. The data are useful for scientific researchers and biotechnologists, e.g., (1) to maintain microbial diversity through detection/comparison of microbial compounds and metabolites and (2) to optimize and control biotechnological processes.
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Kiseleva, E., Mikhailopulo, K., Novik, G. (2018). Modern Immunochemical Approaches in Microbiology. In: Sharma, S., Varma, A. (eds) Microbial Resource Conservation. Soil Biology, vol 54. Springer, Cham. https://doi.org/10.1007/978-3-319-96971-8_11
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