Abstract
The integration of biological tissue and artificial materials plays a fundamental role in the development of biohybrid soft robotics, a subfield in the field of soft robotics trying to achieve a higher degree of complexity by taking advantage of the exceptional capabilities of biological systems, like self-healing or responsiveness to external stimuli. In this work, we present a proof-of-concept 3D bioprinted bio-actuator made of skeletal muscle tissue and PDMS, which can act as a force measuring platform. The 3D bioprinting technique, which has not been used for the development of bio-actuators, offers unique versatility by allowing a simple, biocompatible and fast fabrication of hybrid multi-component systems. Furthermore, we prove controllability of contractions and functionality of the bio-actuator after applying electric pulses by measuring the exerted forces. We observe an increased force output in time, suggesting improved maturation of the tissue, opening up possibilities for force adaptability or modulation due to prolonged electrical stimuli.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Patino, T., Mestre, R., Sánchez, S.: Miniaturized soft bio-hybrid robotics: a step forward into healthcare applications. Lab Chip 16 (2016). https://doi.org/10.1039/c6lc90088g
Raman, R., Cvetkovic, C., Uzel, S.G.M., et al.: Optogenetic skeletal muscle-powered adaptive biological machines. Proc. Natl. Acad. Sci. USA 113, 3497–3502 (2016). https://doi.org/10.1073/pnas.1516139113
Park, S.-J., Gazzola, M., Park, K.S., et al.: Phototactic guidance of a tissue-engineered soft-robotic ray. Science 353, 158–162 (2016). https://doi.org/10.1126/science.aaf4292
Chan, V., Park, K., Collens, M.B., et al.: Development of miniaturized walking biological machines. Sci. Rep. 2, 857 (2012). https://doi.org/10.1038/srep00857
Ricotti, L., Menciassi, A.: Bio-hybrid muscle cell-based actuators. Biomed. Microdevices 14, 987–998 (2012). https://doi.org/10.1007/s10544-012-9697-9
Webster-Wood, V.A., Akkus, O., Gurkan, U.A., et al.: Organismal engineering toward a robotic taxonomic key for devices using organic materials. Sci. Robot., 2, eaap9281 (2017). https://doi.org/10.1126/scirobotics.aap9281
Kang, H.-W., Lee, S.J., Ko, I.K., et al.: A 3D bioprinting system to produce human-scale tissue constructs with structural integrity. Nat. Biotechnol. 34, 312–319 (2016). https://doi.org/10.1038/nbt.3413
Raman, R., Cvetkovic, C., Bashir, R.: A modular approach to the design, fabrication, and characterization of muscle-powered biological machines. Nat. Protoc. 12, 519–533 (2017). https://doi.org/10.1038/nprot.2016.185
Fujita, H., Nedachi, T., Kanzaki, M.: Accelerated de novo sarcomere assembly by electric pulse stimulation in C2C12 myotubes. Exp. Cell Res. 313, 1853–1865 (2007). https://doi.org/10.1016/j.yexcr.2007.03.002
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2018 Springer International Publishing AG, part of Springer Nature
About this paper
Cite this paper
Mestre, R., Patiño, T., Barceló, X., Sanchez, S. (2018). 3D Bioprinted Muscle-Based Bio-Actuators: Force Adaptability Due to Training. In: Vouloutsi , V., et al. Biomimetic and Biohybrid Systems. Living Machines 2018. Lecture Notes in Computer Science(), vol 10928. Springer, Cham. https://doi.org/10.1007/978-3-319-95972-6_33
Download citation
DOI: https://doi.org/10.1007/978-3-319-95972-6_33
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-319-95971-9
Online ISBN: 978-3-319-95972-6
eBook Packages: Computer ScienceComputer Science (R0)