Abstract
Ecological stoichiometry theory helps us to better understand trophic interactions by analyzing the imbalances in the relative supplies of key elements (carbon, nitrogen, and phosphorus) between organisms and their resources. However, the mechanisms that control elemental stoichiometry in different taxonomic groups and the effects of nutrient supply imbalances are not yet clear. Aquatic microfungi are an ecological group of microorganisms ranging from those adapted to complete their life cycles in aquatic habitats to those that occurring in water fortuitously. Aquatic fungi are important regulators of plant productivity, community dynamics, and diversity in nutrient-poor and extreme ecosystems. Because aquatic fungi are heterotrophs, it has been assumed that they possess high degree of stoichiometric homeostasis. However, data concerning their elemental composition and their degree of homeostasis remain scarce. Herein, we analyzed the C:N:P stoichiometry of mycelia in ten aquatic microfungi isolated from three hydrological systems with different nutrient conditions within Cuatro Cienegas Basin (CCB). Our hypotheses were (a) variations in C:N:P ratios reflect divergent life history strategies between the three environments, independently of the fungal taxa involved, and (b) C:N:P ratios reflect physiological adjustments associated with specific taxa, independent of the environmental characteristics. Our results provide some support for the first hypothesis, as the apparent capacity for elemental stoichiometry regulation in the aquatic microfungi was not linked to phylogenetic relationships but appeared to be an adaptation to resource availabilities in the environment in which they grew. The microfungi isolated from the most oligotrophic site within the CCB (Pozas Rojas) most strongly regulated their elemental stoichiometry in comparison with fungal isolates from other sites within CCB.
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References
Andersen T, Hessen DO (1991) Carbon, nitrogen, and phosphorus content of freshwater zooplankton. Limnol Oceanogr 36:807–814
Andersen T, Elser JJ, Hessen DO (2004) Stoichiometry and population dynamics. Ecol Lett 7:884–900
Breitbart M, Hoare A, Nitti A (2009) Metagenomic and stable isotopic analyses of modern freshwater microbialites in Cuatro Cienegas, Mexico. Environ Microbiol 11:16–34. https://doi.org/10.1111/j.1462-2920.2008.01725.x
Bremner JM, Mulvaney CS (1982) Nitrogen-total. In: Page AL, Miller RH, Keeney DR (eds) Methods of soil analysis: chemical and microbiological properties. American Society of Agronomy and Soil Science Society of America, Madison, WI, pp 595–624
Cheever BM, Kratzer EB, Webster JR (2012) Immobilization and mineralization of N and P by heterotrophic microbes during leaf decomposition. Freshw Sci 31:133–147
Cleveland CC, Liptzin D (2007) C:N:P stoichiometry in soil: is there a “Redfield ratio” for the microbial biomass? Biogeochemistry 85:235–252
Cross WF, Wallace JB, Rosemond AD (2007) Nutrient enrichment reduces constraints on material flows in a detritus-based food web. Ecology 88:2563–2575. https://doi.org/10.1890/06-1348.1
Danger M, Chauvet E (2013) Elemental composition and degree of homeostasis of fungi: are aquatic hyphomycetes more like metazoans, bacteria or plants? Fungal Ecol 6:453–457
Dighton J (1997) Nutrient cycling by saprotrophic fungi in terrestrial habitats. Mycota 4:271–279
Duarte S, Bärlocher F, Trabulo J et al (2015) Stream-dwelling fungal decomposer communities along a gradient of eutrophication unraveled by 454 pyrosequencing. Fungal Divers 70:127–148
Elser JJ, Urabe J (1999) The stoichiometry of consumer-driven nutrient recycling: theory, observations and consequences. Ecology 80:735–751
Elser JJ, Sterner RW, Gorokhova E et al (2000a) Biological stoichiometry from genes to ecosystems. Ecol Lett 3:540–550. https://doi.org/10.1111/j.1461-0248.2000.00185.x
Elser JJ, O’Brien WJ, Dobberfuhl DR et al (2000b) The evolution of ecosystem processes: growth rate and elemental stoichiometry of a key herbivore in temperate and arctic habitats. J Evol Biol 13:845–853. https://doi.org/10.1046/j.1420-9101.2000.00215.x
Elser JJ, Schampel JH, Garcia-Pichel F et al (2005) Effects of phosphorus enrichment and grazing snails on modern stromatolitic microbial communities. Freshw Biol 50:1808–1825. https://doi.org/10.1111/j.1365-2427.2005.01451.x
Escalante AE, Eguiarte LE, Espinosa-Asuar L et al (2008) Diversity of aquatic prokaryotic communities in the Cuatro Cienegas basin. FEMS Microbiol Ecol 65:50–60. https://doi.org/10.1111/j.1574-6941.2008.00496.x
Fierer N, Bradford MA, Jackson RB (2007) Toward an ecological classification of soil bacteria. Ecology 88:1354–1364. https://doi.org/10.1890/05-1839
Frost PC, Michelle A, Evans-White Z et al (2005) Are you what you eat? Physiological constraints on organismal stoichiometry in an elementally imbalanced world. Oikos 109:18–28
Huffman EWD (1977) Performance of a new automatic carbon dioxide coulometer. Microcheml J 22(4):567–573
Koojiman SALM (1995) The stoichiometry of animal energetics. J Theor Biol 177:139–149
Lee ZM, Steger L, Corman JR et al (2015) Response of a stoichiometrically imbalanced ecosystem to manipulation of nutrient supplies and ratios. PLoS One 10(4):e0123949. https://doi.org/10.1371/journal.pone.0123949
López-Lozano NE, Eguiarte LE, Bonilla-Rosso G et al (2012) Bacterial communities and the nitrogen cycle in the gypsum soil of Cuatro Ciénegas Basin, Coahuila: a Mars analogue. Astrobiology 12:699–709. https://doi.org/10.1089/ast.2012.0840
Minckley WL, Cole GA (1968) Preliminary limnologic information on waters of the Cuatro Cienegas Basin, Mexico. Southwest Nat 13:421–431. https://doi.org/10.2307/3668909
Murphy J, Riley JP (1962) A modified single solution method for the determination of phosphorus in natural water. Anal Chim Acta 27:31–36
Pastor A, Compson ZG, Dijkstra P et al (2014) Stream carbon and nitrogen supplements during leaf litter decomposition: contrasting patterns for two foundation species. Oecologia 176:1111–1121. https://doi.org/10.1007/s00442-014-3063-y
Peimbert M, Alcaraz LD, Bonilla-Rosso G et al (2012) Comparative metagenomics of two microbial mats at Cuatro Ciénegas Basin I: ancient lessons on how to cope with an environment under severe nutrient stress. Astrobiology 12:648–658. https://doi.org/10.1089/ast.2011.0694
Persson J, Fink P, Goto A et al (2010) To be or not to be what you eat: regulation of stoichiometric homeostasis among autotrophs and heterotrophs. Oikos 119:741–751. https://doi.org/10.1111/j.1600-0706.2009.18545.x
Redfield AC (1958) The biological control of chemical factors in the environment. Am Sci 46:205–221
Schimel JP, Bennett J (2004) Nitrogen mineralization: challenges of a changing paradigm. Ecology 85:591–602. https://doi.org/10.1890/03-8002
Schneider K, Renker C, Maraun M (2005) Oribatid mite (Acari, Oribatida) feeding on ectomycorrhizal fungi. Mycorrhiza 16:67–72
Scott EE, Prater C, Norman E et al (2013) Leaf-litter stoichiometry is affected by streamwater phosphorus concentrations and litter type. Freshw Sci 32:753–761. https://doi.org/10.1899/12-215.1
Shearer CA, Descals E, Kohlmeyer B et al (2007) Fungal biodiversity in aquatic habitats. Biodivers Conserv 16:49–67. https://doi.org/10.1007/s10531-006-9120-z
Souza V, Espinosa-Asuar L, Escalante AE et al (2006) An endangered oasis of aquatic microbial biodiversity in the Chihuahuan desert. PNAS 103:6565–6570. https://doi.org/10.1073/pnas.0601434103
Sterner RW, Elser JJ (2002) Ecological stoichiometry: the biology of elements from molecules to the biosphere. Princeton University Press, Princeton, NJ
Sterner RW, Clasen L et al (1998) Carbon: phosphorus stoichiometry and food chain production. Ecol Lett 1:146–150
Tapia-Torres Y, Elser JJ, Souza V et al (2015) Ecoenzymatic stoichiometry at the extremes: how microbes cope in an ultra-oligotrophic desert soil. Soil Biol Biochem 87:34–42 https://doi.org/10.1016/j.soilbio.2015.04.007
Tapia-Torres Y, Rodríguez-Torres D, Islas A et al (2016) How to live with phosphorus scarcity in soil and sediments: lessons from bacteria. Appl Environ Microbiol 82:4652–4662
Tobler M, Carson EW (2010) Environmental variation, hybridization, and phenotypic diversification in Cuatro Cienegas pupfishes. J Evol Biol 23:1475–1489
Valdivia-Anistro JA, Eguiarte-Fruns LE, Delgado-Sapién G et al (2016) Variability of rRNA operon copy number and growth rate dynamics of Bacillus isolated from an extremely oligotrophic aquatic ecosystem. Front Microbiol 6:1486. https://doi.org/10.3389/fmicb.2015.01486
Van Der Heijden MG, Bardgett RD, Van Straalen NM (2008) The unseen majority: soil microbes as drivers of plant diversity and productivity in terrestrial ecosystems. Ecol Lett 11:296–310. https://doi.org/10.1111/j.1461-0248.2007.01139.x
Velez P, Gasca-Pineda J, Rosique-Gil E et al (2016) Microfungal oasis in an oligotrophic desert: diversity patterns and community structure in three freshwater systems of Cuatro Ciénegas, Mexico. PeerJ 4:e2064
Velez P, Espinosa-Asuar L, Figueroa M et al (2018 Under revision) Nutrient dependent cross-kingdom interactions: microfungi and bacteria from an oligotrophic desert oasis. Front Microbiol (Under revision)
Zhang J, Elser JJ (2017) Carbon:nitrogen:phosphorus stoichiometry in fungi: a meta-analysis. Front Microbiol 8:1281. https://doi.org/10.3389/fmicb.2017.01281
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Tapia-Torres, Y., Vélez, P., García-Oliva, F., Eguiarte, L.E., Souza, V. (2018). The Effect of Nutrient Availability on the Ecological Role of Filamentous Microfungi: Lessons from Elemental Stoichiometry. In: García-Oliva, F., Elser, J., Souza, V. (eds) Ecosystem Ecology and Geochemistry of Cuatro Cienegas. Cuatro Ciénegas Basin: An Endangered Hyperdiverse Oasis. Springer, Cham. https://doi.org/10.1007/978-3-319-95855-2_4
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